In the Mao lab we are interested in understanding how tissues achieve their correct size, shape and complex three-dimensional architecture, both during normal development, and during regenerative growth.
The genetic and biochemical control of tissue growth and regeneration has been extensively studied over the last century, but it is still unclear how the physical and mechanical properties of cells and tissues contribute to how organs are formed and sculpted. What is clear is that in order to change the three-dimensional architecture of any structure, there must be forces, external and/or internal, acting on the system.
We use an interdisciplinary approach, combining Drosophila genetics, live imaging, automated image analysis, experimental biophysics, engineering and computational modeling, to understand the importance of mechanical forces in controlling tissue growth and regeneration and how these forces in turn influence gene expression and signaling pathways.
Heller D, et al (2016). EpiTools: An Open-Source Image Analysis Toolkit for Quantifying Epithelial Growth Dynamics. Developmental Cell, 36 (1), 103-116. doi:10.1016/j.devcel.2015.12.012
Sanchez-Gutierrez D, et al (2015). Fundamental physical cellular constraints drive self-organization of tissues. EMBO JOURNAL, 35 (1), 77-88. doi:10.15252/embj.201592374
Mao Y & Baum B (2015). Tug of war-The influence of opposing physical forces on epithelial cell morphology.DEVELOPMENTAL BIOLOGY, 401 (1), 92-102. doi:10.1016/j.ydbio.2014.12.030
Mao Y, et al (2013). Differential proliferation rates generate patterns of mechanical tension that orient tissue growth.The EMBO Journal, 32 (21), 2790-2803. doi:10.1038/emboj.2013.197
Mao Y, et al (2011). Planar polarization of the atypical myosin Dachs orients cell divisions in Drosophila. Genes & Development, 25 (2), 131-136. doi:10.1101/gad.610511
Cytoskeleton and cell cortex, Polarity and cell shape, Signalling pathways, Cell-cell interactions, Physics of biological systems, Tissue growth and morphogenesis, Tissue repair and regeneration
Light microscopy, Electron microscopy, Computational modeling, Super-resolution microscopy
Robert Tetley (Postdoctoral Fellow)
Alice Roycroft (Research Associate)
Nargess Khalilgharibi (Research Associate)
Natalie Kirkland (PhD Student)
Ricardo Barrientos (PhD Student)
Filippos Ioannou (PhD Student)
Alejandra Guzman-Herrera (PhD student)