Light microscopy

Light microscopy is of critical importance in cell biology. The MRC LMCB Light Microscopy Facility is provided as a service to researchers, with light microscopy staff members maintaining the microscopes, training users, providing hands-on help and instruction in microscope techniques and investigating developments in microscope methods and technology. Light microscopy staff members will also contribute directly to research by assisting in experimental strategy and design, and by devising and carrying out procedures that require expert skills.

Microscopes and image analysis software

The light microscopes in the MRC LMCB include eight confocal microscopes (including a spinning disc confocal for live imaging); two time-lapse microscope systems and one TIRF time-lapse, all with chambers for sample environmental control; and five research fluorescence microscopes. A full range of open-source and commercial image processing and analysis software is supported.

Training, booking and other policies and resources

Staff members in the Light Microscopy Facility provide introductory and advanced training on both microscopes and imaging software. Please see the information below to find out how to book training sessions and to see the facility's access and data policies. There is also a link to a calendar listing UK and worldwide courses and conferences in the field of light microscopy.

Charge for access

The LMCB Light Microscopy Facility has to recover its costs so you must include the charge for access to the facility in any funding application. The facility manager will be able to help you estimate how much funding is required based on the hourly charge and an assessment of the total number of hours needed. Please contact the facility manager for advice and information about the hourly charge rates as soon as you begin applying for funding.

Light Microscopy Facility contact details

Please make any enquiries about training, help with projects or problems on machines using the following email address:

Alternatively you can contact the relevant member of facility staff directly. Please see the table below for details:

Staff Member Position Key Responsibilities Room Number Extension
Andrew Vaughan Facility Manager GFP1, GFP2, GFP4, Vox, Radiance 1.13 37904
John Gallagher Light Microscopy Officer SPE, SPE2, SPE3, SP5 G.13 32295
Ki Hng Light Microscopy Officer FV1200, Elyra, NSTORM, STED, GFP3 3.04 37916


UCL Super-Resolution Facility (SuRF)

The LMCB is home to the UCL Super-Resolution Facility, funded by the MRC Next Generation Optical Microscopy initiative. The LMCB facility consists of a microscope development lab, headed up by Ricardo Henriques and a general SuRF facility consisting of two commercially acquired super-resolution microscopes - a Leica Microsystems gSTED and a Carl Zeiss Elyra PS.1. To access the facility you must submit a project via the Facility Access link on the SuRF Homepage below:

SuRF Homepage