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Ricardo Henriques's picture

LMCB Group Leader, UCL Senior Lecturer, Experimental Optics Leader

r.henriques@ucl.ac.uk
+44 (0)20 7679 7936
LMCB Room 4.13

 

Quantitative Imaging and Nanobiophysics

Research Synopsis

The Henriques lab was established in 2013 to undertake research combining cell biology, optical physics and biochemistry. The group focuses on biological problems that cannot be addressed with current imaging technology, and thus aims to develop analytical, optical and biochemical approaches to address these questions. In cell biology we aim to understand how viruses enter cells by probing and remodelling membranes, and what are the structural changes viruses undergo during cell-entry, uncoating and morphogenesis. To do so, we are developing new classes of fluorescent probes, high-speed cell friendly Super-Resolution (SR) methods and computational modelling approaches that, although designed to answer questions of interest in the lab, will have broad applications in cell biology research.

 

Selected Publications

Gustafsson N, et al (2016). Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations. Nature Communications, 7. doi:10.1038/ncomms12471
Scholefield J, et al (2016). Super-resolution microscopy reveals a preformed NEMO lattice structure that is collapsed in incontinentia pigmenti. Nature Communications, 7, 12629. doi:10.1038/ncomms12629
Gray RDM, et al (2016). VirusMapper: Open-source nanoscale mapping of viral architecture through super-resolution microscopy. Scientific Reports, 6. doi:10.1038/srep29132
Soares H, et al (2013). Regulated vesicle fusion generates signaling nanoterritories that control T cell activation at the immunological synapse. J Exp Med, 210 (11), 2415-2433. doi:10.1084/jem.20130150
Lelek M, et al (2012). Superresolution imaging of HIV in infected cells with FlAsH-PALM. Proc Natl Acad Sci U S A, 109 (22), 8564-8569. doi:10.1073/pnas.1013267109
Henriques R, et al (2010). QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ. Nat Methods, 7 (5), 339-340. doi:10.1038/nmeth0510-339

Funders

Medical Research Council
Wellcome Trust
BBSRC

Research Themes

Cytoskeleton and cell cortex, Signalling pathways, Cell-cell interactions, Membrane trafficking, Viral pathogens, Biophysics, Computational biology

Technology

Light microscopy, Super-resolution microscopy, Translational research, Electron Microscopy

People

Pedro Pereira (Postdoctoral Fellow)
Siân Culley (Postdoctoral Fellow)
David Albrecht (Postdoctoral Fellow)
Caron Jacobs (PhD Student, Commonwealth Studentship)
Nils Gustafsson (PhD Student, UCL CoMPLEX Studentship)
Pedro Almada (PhD Student, BBSRC LiDO Studentship)
Robert Gray (PhD Student, UCL CoMPLEX Studentship)
Jerzy Samolej (PhD Student, BBSRC LiDO Studentship)

Collaborators

Mark March (LMCB, UK)
Jason Mercer (LMCB, UK)
Buzz Baum (LMCB, UK)
Joe Grove (UCL, UK)
Dylan Owen (KCL, UK)
Jonas Ries (EMBL, Germany)
Musa Mhlanga (UCT, South Africa)
Helena Soares (CEDOC, Portugal)
Ralf Jungmann (MPI Biochemistry, Germany)