Centre for Amyloidosis and Acute Phase Proteins


Histology and Proteomic Analysis (LDMS)

Accurate identification of the amyloid fibril protein is the critical first step in the diagnostic pathway for any patient with amyloidosis.

At the Jack O'Neill Laboratory, we perform Congo red staining to confirm amyloid deposition.

Amyloid fibril typing is done by a panel of specialised antibodies to a variety of amyloid fibril proteins using immunohistochemical methods optimised for amyloid fibril typing.  In cases where the immunohistochemistry is not decisive, or additional information is considered useful for clinical or diagnostic purposes, samples are further analysed by laser capturing the amyloid deposits and subjecting them to mass spectrometry (LCMS), a sophisticated and highly accurate approach to determine the amyloid fibril type.

For any questions regarding histology or proteomic analysis please contact Janet Gilbertson tel +44 (0)20 7433 2752.

Any clinical advice about amyloid typing must be discussed with one of our consultant staff on tel +44 (0)20 7433 2811 or by email to: Professor Julian Gillmore,  Professor Helen Lachmann, Professor Ashutosh Wechalekar or Professor Philip Hawkins.