3D-LiveSRRF: Open-Source Live-Cell 3D Super-Resolution Microscopy.

Many key cellular processes are commonly studied by quantitative biochemical methods. These, however, do not typically allow for the direct observation of  dynamic interaction of molecular partners involved in their native state, in living cells. Being able to directly observe molecular assemblies in cellular systems at the relevant spatial and temporal scales would allow us to discern the behaviour of molecular mechanisms governing cellular function and would have long-reaching potential for biomedical research.

The recently developed Super-resolution microscopy (SRM) approaches have now made it possible to image cellular systems with resolution beyond the diffraction limit of conventional microscopy. However, these techniques typically require long acquisition times and high illumination intensities onto the sample that are highly phototoxic and therefore limit the possibilities for live-cell imaging. Additionally, another active field of research for SRM is the development of methods that extend the resolution improvement to 3D volumes, including the axial direction. Due to the inherent 3D architectures of numerous molecular assemblies in cells, it is important to achieve true super-resolution in 3D.

Engaging with these new 3D imaging tools is a key aspect of this project. The intent is to develop new methodologies and best practices within a team of researchers based at UCL and Paris.

Area

Biology

UCL lead

• Dr Romain Laine, Labratory for Moleculary Cell Biology