Inverted Zeiss LSM 710 confocal

Book on PPMS.

User Guides:

Links to start-up / shutdown & reboot procedures and the ZEN 2009 quick start tutorial (tutorial update in progress).

System Overview:

Zeiss LSM 710 inverted confocal microscope.

7 laser lines for excitation: 405; 458; 488; 514; 561; 594 and 633 nm (check online or ask Dale if you are not sure which dye to use).

Objectives (DIC on all objectives):

• 10x Plan Apochromat NA 0.45 WD 2000 um Dry
• 20x Plan Apochromat NA 0.8 WD 550 um Dry
• 25x LD LCI Plan Apochromat NA 0.8 WD 570 um corrected Multi-immersion
• 40x C-Apochromat NA 1.2 WD 280 um Water
• 63x Plan-Apochromat NA 1.4 WD 190 um Oil

Location:

Room 310
ICH

Features:

• Z sectioning for 3D imaging
• Continuous Spectral imaging (from 390 nm to 750 nm down to 3 nm)
• Transmitted light detector (DIC objectives)
• FRET and FRAP
• Live imaging (CO2, humidity and temperature controlled chamber)
• Multi-point experiments  (XYZ positions, tiling)

Cost:

• User operation £28 per hour, reduced rate £20 at weekends
• Non-ICH academic rate: £35 per hour, £25 weekends
• £10 per hour of overnight time-lapse
• Staff operation £50 per hour
• Training session £50 per hour