Protocols and Publishing Advice

Some protocols commonly used in the Towers lab

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  Publication Advice Guide - Record Keeping

  Publication Advice Guide - Record Keeping

• Retrovirus prep by transfection of 293Ts

  Retrovirus prep by transfection of 293Ts

• Removal of Plasmid DNA from virus preps by DNAse treatment

  Removal of Plasmid DNA from virus preps by DNAse treatment

• Virus purification through 25% sucrose

  Virus purification through 25% sucrose

• Preparation of Competent Bacteria

  Preparation of Competent Bacteria

• Intracellular staining of human NF-kB in adherent cells

  Intracellular staining of human NF-kB in adherent cells

We use Bluetractor Software's DNA Dynamo for DNA analysis, oligo design, plasmid sequence management and plasmid maps.

We use the Hermes High Definition Cell Imaging technology for High Content Screening.