UCL Great Ormond Street Institute of Child Health


Great Ormond Street Institute of Child Health


Widefield Imaging


Training is required before using facility equipment, please register and request training on PPMS.

Training is free for ICH / GOSH users. £50 per hour for other UCL users.

Fluorescence imaging - Book on PPMS.

  • #1 Leica upright manual 3 colour fluorescence scope with IDS 3260CP monochrome camera. High NA objectives (10x/0.4, 20x/0.7, 63x/1.2Water, 63x/1.4Oil, 100x/1.4). Good for slides. Link to user guide.
  • Olympus IX71 inverted TC scope with Hamamatsu Orca R2 monochrome camera. For cells in TC plates flasks and dishesLink to user guide.

Brightfield imaging (i.e H&E, DAB etc) - Book on PPMS.

Scope 2 can also do fluorescecne, but the scopes above are far better for fluorescence. These scopes are best for colour imaging of histology slides.

  • #2 Zeiss Axioplan2 - Zeiss Axiocam HRc colour camera (donated by NDU). Polarization contrast scope, see image below.
  • #5 Zeiss Axioplan2 - Zeiss Axiocam HRc colour camera (donated by MMU/NU).
  • Link to Kohler set up scope #2 (microscope set up) & Link to software guide.


Widefield imaging facilities are on the 3rd floor Main Building, rooms 310, 301 and 304. 

Operating instructions:

Follow links above. Also available: pixel sizes for scale bars and details of filter sets are in the Advice and Protocols pages.


detailed here

Picrosirius Red staining of collagen fibres under normal and polarized light.

MediaCentral Widget Placeholderhttps://mediacentral.ucl.ac.uk/flash/31476732_hd.mp4

Polarization contrast highlights birefringent materials.