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Training:
Training is required before using facility equipment, please register and request training on PPMS.
Training is free for ICH / GOSH users. £50 per hour for other UCL users.
Fluorescence imaging - Book on PPMS.
- #1 Leica upright manual 3 colour fluorescence scope with IDS 3260CP monochrome camera. High NA objectives (10x/0.4, 20x/0.7, 63x/1.2Water, 63x/1.4Oil, 100x/1.4). Good for slides. Link to user guide.
- #3 Zeiss Observer inverted fully automated 7 colour fluorescence (Link to filtersets and spectral profiles on FPBase). Hamamatsu Flash 4v3 camera, High NA objectives (5x/0.16 10x/0.45 20x/0.8 40x/1.3 63x/1.4). Great for slides, tilescans, z-stacks, deconvolution. Link to user guide.
- Olympus IX71 inverted TC scope with Hamamatsu Orca R2 monochrome camera. For cells in TC plates flasks and dishes. Link to user guide.
Brightfield imaging (i.e H&E, DAB etc) - Book on PPMS.
Scope 2 can also do fluorescecne, but the scopes above are far better for fluorescence. These scopes are best for colour imaging of histology slides.
- #2 Zeiss Axioplan2 - Zeiss Axiocam HRc colour camera (donated by NDU). Polarization contrast scope, see image below.
- #5 Zeiss Axioplan2 - Zeiss Axiocam HRc colour camera (donated by MMU/NU).
- Link to Kohler set up scope #2 (microscope set up) & Link to software guide.
Location:
Widefield imaging facilities are on the 3rd floor Main Building, rooms 310, 301 and 304.
Operating instructions:
Follow links above. Also available: pixel sizes for scale bars and details of filter sets are in the Advice and Protocols pages.
Charges:
Picrosirius Red staining of collagen fibres under normal and polarized light.
Polarization contrast highlights birefringent materials.
