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Publications

2014

Perkins, S. J., Fung, K.-W. & Khan, S. (2014). Molecular interactions between complement factor H and its heparin and heparan sulphate ligands. Frontiers in Immunology. 5, 126, 1-14. Pubmed 24744754.

2013

Perkins S. J., Nan, R., Li, K. & Khan, S. (2013). Complement factor H and its ligands – a multidisciplinary approach to protein modeling. Encyclopedia of Biophysics, G. C. K. Roberts (Editor). 22. Molecular Shape and Hydrodynamics. pp  1-11. DOI: 10.1007/SpringerReference_308330

Rayner, L. E., Kadkhodayi-Kholghi, N., Heenan, R. K., Gor, J., Dalby, P. A. & Perkins S. J. (2013). The solution structure of rabbit IgG accounts for its interactions with the Fc receptor and complement C1q and its conformational stability. J. Mol. Biol. 425, 506-523. Pubmed 23178865.

Nan, R., Tetchner, S., Rodriguez, E., Pao, P.-J., Gor, J., Lengyel, I. & Perkins, S. J. (2013). Zinc-induced self-association of complement C3b and factor H: implications for inflammation and age-related macular degeneration. J. Biol. Chem. 288, 19197-19210. Pubmed 23661701.

Rallapalli, P. M., Kemball-Cook, G., Tuddenham, E. G., Gomez, K. & Perkins, S. J. (2013). A new interactive web database of mutations in coagulation factor IX provides novel insights into the phenotypes and genetics of haemophilia B. J. Thromb. Haemost. 11, 1329-1340. Pubmed 23617593.

Khan, S., Fung, K.-W., Rodriguez, E., Patel, R., Gor, J., Mulloy, B. & Perkins, S. J. (2013). The solution structure of heparan sulphate differs from that of heparin: implications for function. J. Biol. Chem. 288, 27737-27751. Pubmed 23921391.

Islam, M., Gor, J., Perkins, S. J., Ishikawa, Y., Bächinger, H. P. & Hohenester, E. (2013). The concave face of decorin mediates reversible dimerisation and collagen binding. J. Biol. Chem. 288, 35526–35533. Pubmed 24169694.

2012

Mulloy, B., Khan, S. and Perkins, S. J. (2012). The molecular architecture of heparin and heparan sulfate: recent developments in solution structural studies. Pure and Applied Chemistry. 84, 65-76.

Perkins, S. J., Nan, R., Li, K., Khan, S. & Miller, A. (2012). Complement factor H-ligand interactions: self-association, multivalency and dissociation constants. Immunobiology. 217, 281-297. Pubmed 22137027.

Miller, A., Phillips, A., Gor, J., Wallis, R. & Perkins, S. J. (2012). The near-planar solution structures of mannose-binding lectin oligomers provide insight on the activation of the lectin pathway of complement. J. Biol. Chem. 287, 3930-3945. Pubmed 22167201

Li, K., Gor, J., Holers, V. M., Storek, M. J. & Perkins, S. J. (2012). Solution structure of TT30, a novel therapeutic agent for complement-mediated diseases, provides insight on its joint binding to complement C3b and C3d. J. Mol. Biol. 418, 248-263. Pubmed 22387467.

Khan, S., Nan, R., Gor, J., Mulloy, B. & Perkins, S. J. (2012). Bivalent and co-operative binding of complement Factor H to heparan sulphate and heparin. Biochem. J. 444, 417-428. Pubmed 22471560.

2011

Perkins, S. J. (2011). Invited review. New results may help stop age-related macular degeneration at the molecular level. ESRF Highlights 2010, 61-62.

Nan, R., Farabella, I., Schumacher, F., Miller, A., Gor, J., Martin, A.C.R., Jones, D.T., Lengyel, I. & Perkins, S. J. (2011). Localisation of the major zinc-binding site of complement Factor H to the SCR-6/8 domains: possible implications for age-related macular degeneration. J. Mol. Biol. 408, 714-735.  Pubmed 21396937.

Perkins, S. J., Nan, R., Li, K., Khan, S. & Abe, Y. (2011). Analytical ultracentrifugation combined with X-ray and neutron scattering: experiment and modeling. Modern Analytical Ultracentrifugation Methods (Eds. Schuck, P. & Zhao, J.), Methods, 54, 181-199. Pubmed 21256219.

Khan, S., Rodriguez, E., Patel, R., Gor, J., Mulloy, B. & Perkins, S. J. (2011). The solution structure of heparan sulphate differs from that of heparin: implications for function. J. Biol. Chem. 286, 24842–24854.  Pubmed 21576246.

2010  

Abe, Y., Gor, J., Bracewell, D. G., Perkins, S. J. & Dalby, P. A. (2010). Masking of the Fc region in human IgG4 by constrained X-ray scattering modelling: implications for antibody function and therapy.  Biochem. J. 432, 101-111. Pubmed 20722630.

Okemefuna, A. I., Stach, L., Rana, S., Ziai Buetas, A. J., Gor, J. & Perkins, S. J. (2010). C-reactive protein exists in an NaCl concentration dependent pentamer-decamer equilibrium in physiological buffer. J. Biol. Chem. 285, 1041-1052. Pubmed 19903811

Okemefuna, A. I., Nan, R., Miller, A. Gor, J. & Perkins, S. J. (2010) Complement Factor H binds at two independent sites to C-reactive protein in acute-phase concentrations. J. Biol. Chem. 285, 1053-1065. JBC Paper of the Week Pubmed 19850925

Khan, S., Gor, J., Mulloy, B. & Perkins, S. J. (2010). Semi-rigid solution structures of heparin by constrained X-ray scattering modelling: new insight into heparin-protein complexes. J. Mol. Biol. 395, 504-521. Pubmed 19895822

Hebecker, M., Okemefuna, A. I., Perkins, S. J., Mihlan, M. & Józsi, M. (2010). Molecular basis of C-reactive protein binding and modulation of complement activation by factor H-related protein 4. Molec. Immunol. 47, 1347-1355. Pubmed 20042240

Johnson, S. A., Williams, J. M., Hakobyan, S., Richards, A., Perkins, S. J., Marchbank, K. J., Goodship, T. H. J., Morgan, B. P., Taylor, C. M. & Savage, C. O. S. (2010). Impact of compound heterozygous complement factor H mutations on development of atypical hemolytic uremic syndrome – a pedigree revisited. Molec. Immunol. 47, 1585-1591. Pubmed 20304497

Leonard, P. G., Parkinson, G. N., Gor, J., Perkins, S. J. & Ladbury, J. E. (2010). The absence of inorganic salt is required for the crystallization of the complete oligomerization domain of Salmonella typhimurium histone-like nucleoid-structuring protein. Acta Cryst. F66, 421-466. Pubmed 20383013

Perkins, S. J., Nan, R., Okemefuna, A. I., Li, K., Khan, S. & Miller, A. (2010). Multiple interactions of complement factor H with its ligands in solution: a progress report. Current Topics on Complement and Eye Diseases (Ed. J.D. Lambris and A. Adamis).
Adv. Exp. Med. Biol. 703, 25-47. Pubmed 20711705.

Perkins, S. J., Okemefuna, A. I., & Nan, R. (2010). Unravelling protein-protein interactions between complement factor H and C-reactive protein by a multidisciplinary strategy. Biochem. Soc. Transact. 38, 894-900. PubMed 20658973.

Niewiarowski, A., Bradley, A. S., Gor, J., McKay, A. R., Perkins, S. J. & Tsaneva, I. R. (2010). Oligomeric assembly and interactions within human RuvBL1 and RuvBL2 complexes. Biochem. J. 429, 113-125. Pubmed 20412048

Tchepeleva, S. N., Thurman, J. M., Ruff, K., Perkins, S. J., Morel, L. & Boackle, S. A. (2010). An allelic variant of Crry in the murine Sle1c lupus susceptibility interval is not impaired in its ability to regulate complement activation. J. Immunol. 185, 2331-2339. Pubmed 20660348. 

Li, K., Gor, J. & Perkins, S. J. (2010). Self-association and domain rearrangements between complement C3 and C3u provide insight into the activation mechanism of C3. Biochem. J. 431, 63-72. Pubmed 20666732.

2009

Bonner, A., Almogren, A., Furtado, P. B., Kerr, M. A. & Perkins, S. J. (2009). Location of secretory component on the Fc edge of dimeric IgA1 reveals insight into the role of secretory IgA1 in mucosal immunity. Mucosal Immunology (Nature Publishing Group), 2, 74-84. Pubmed 19079336

Bonner, A., Almogren, A., Furtado, P. B., Kerr, M. A. & Perkins, S. J. (2009). The non-planar secretory IgA2 and near-planar secretory IgA1 solution structures rationalize their different mucosal immune responses. J. Biol. Chem. 284, 5077-5087. Pubmed 19109255

Almogren, A., Bonner, A., Perkins, S. J. & Kerr, M. A. (2009). Functional and structural characterisation of human colostrum free secretory component. Molecular Immunology, 46, 1534-1541. Pubmed 19230975

Okemefuna, A. I., Nan, R., Gor, J. & Perkins, S. J. (2009). Electrostatic interactions contribute to the folded-back conformation of wild-type human Factor H. J. Mol. Biol. 391, 98-118. Pubmed 19505476

Okemefuna, A. I., Li, K., Nan, R., Ormsby, R. J., Sadlon, T., Gordon, D. L. & Perkins, S. J. (2009). Multimeric interactions between complement Factor H and its C3d ligand provide new insight on complement regulation. J. Mol. Biol. 391, 119-135. Pubmed 19505474

Perkins, S. J. (2009). Unravelling antibody and complement structures in immunity using TS-2 neutrons at ISIS: Neutron scattering. The Biochemist. 31 (4), 32-35.

Saunders, R. E., Shiltagh, N., Gomez, K., Mellars, G., Cooper, C., Perry, D. J. Tuddenham, E. G., & Perkins, S. J. (2009). Structural analysis of eight novel and 111 previously reported missense mutations in the interactive FXI mutation database reveals new insight on FXI deficiency. Thromb. Haemost. 102, 287-301. Pubmed 19652879 (doi:10.1160/TH09-01-0044)

Leonard, P. G., Ono, S., Gor, J., Perkins, S. J. & Ladbury, J. E. (2009). Investigation of the self-association and hetero-association interactions of H-NS and StpA from Enterobacteria. Molecular Microbiology. 73, 165-179. Pubmed 19508284

Perkins, S. J., Okemefuna, A. I., Nan, R., Li, K. & Bonner, A. (2009). Constrained solution scattering modelling of human antibodies and complement proteins reveals novel biological insights. J. Roy. Soc. Interface. 6, S679-S696. Pubmed 19605402

2008

 Perkins, S. J., Okemefuna, A. I., Fernando, A. N., Bonner, A., Gilbert, H. E. & Furtado, P. B. (2008). Invited review. X-ray and neutron scattering data and their constrained molecular modelling. In “Biophysical Tools for Biologists” (eds. Correia, J. J. and Dietrich III, H. W.) Methods Cell Biology, 84, 375-423. Pubmed 17964938

Okemefuna, A. I., Gilbert, H. E., Griggs, K. M., Ormsby, R. J., Gordon, D. L. & Perkins, S. J. (2008). The regulatory SCR-1/5 and cell-surface-binding SCR-16/20 fragments of Factor H reveal partially folded-back solution structures and different self-associative properties. J. Mol. Biol. 375, 80-101. Pubmed 18005991

Furtado, P. B., Huang, C. Y., Ihyembe, D., Hammond, R. A., Marsh, H. C. & Perkins, S. J. (2008). The partly-folded back solution structure arrangement of the 30 SCR domains in human complement receptor type 1 (CR1) permits access to its C3b and C4b ligands. J. Mol. Biol. 375, 102-118. Pubmed 18028942

Nan, R., Gor, J. & Perkins, S. J. (2008). Implications of the progressive self-association of wild-type human Factor H for complement regulation and disease. J. Mol. Biol. 375, 891-900. Pubmed 18054958

Bonner, A., Furtado, P. B., Almogren, A., Kerr, M. A. & Perkins, S. J. (2008). Implications of the near-planar solution structure of human myeloma dimeric IgA1 for mucosal immunity and IgA nephropathy. J. Immunol. 180, 1008-1018. Pubmed 18178841

Perkins, S. J. & Bonner, A. (2008). Structure determinations of human and chimaeric antibodies by solution scattering and constrained molecular modelling. Biochem. Soc. Transact. 36, 37-42. Pubmed 18208381

Saunders, R. E. & Perkins, S. J. (2008) CoagMDB: A database analysis of missense mutations within four conserved domains in five Vitamin K-dependent coagulation serine proteases using a text-mining tool. Human Mutation, 29, 333-344. Pubmed 18058827

Edey, M. M., Mead, P. A., Saunders, R. E., Perkins, S. J., Goodship, T. H. J. & Kanagasundaram, N. S. (2008). Association of Factor H mutation with hemolytic uremic syndrome following a diarrheal illness. Am. J. Kidney Disease. 51, 487-490. Pubmed 18295065

Li, K., Okemefuna, A. I., Gor, J., Hannan, J. P., Asokan, R., Holers, V. M. & Perkins, S. J. (2008) Solution structure of the complex formed between human complement C3d and full length complement receptor Type 2. J. Mol. Biol. 384, 137-150. Pubmed: 18804116

Nan, R., Gor, J., Lengyel, I. & Perkins, S. J. (2008). Uncontrolled zinc- and copper-induced oligomerisation of the human complement regulator Factor H and its possible implications for function and disease. J. Mol. Biol. 384, 1341-1352. Pubmed: 18976665

2007

Saunders, R. E., Abarrategui-Garrido, C., Frémeaux-Bacchi, V., Goicoechea de Jorge, E., Goodship, T. H. J., López Trascasa, M., Noris, M., Ponce Castro, I. M., Remuzzi, G., Rodríguez de Córdoba, S., Sánchez-Corral, P., Skerka, C., Zipfel, P. F. & Perkins, S. J. (2007). The interactive Factor H - atypical Haemolytic Uraemic Syndrome mutation database and website: Update and integration of Membrane Cofactor Protein and Factor I mutations with structural models. Human Mutation, 28, 222-234. Pubmed 17089378

Fernando, A. N., Furtado, P. B., Clark, S. J., Gilbert, H. E., Day, A. J., Sim, R. B. & Perkins, S. J. (2007). Associative and structural properties of the region of complement Factor H encompassing the Tyr402His disease-related polymorphism and its interactions with heparin. J Mol Biol. 368, 564-581. Pubmed 17362990

Barlow, P. N., Lea, S. M. & Perkins, S. J. (2007) Structural analysis of complement proteins: a brief comparison of available approaches. Focus On Complement. Issue 6. Pages 2-5.

Bonner, A., Perrier, C., Corthésy, B. & Perkins, S. J. (2007). Solution structure of human secretory component and implications for biological function. J. Biol. Chem. 282, 16969 - 16980.

2006

Saunders, R. E., Goodship, T. H. J., Zipfel, P. F. & Perkins, S. J. (2006) Factor H-associated haemolytic uraemic syndrome: a web database of the structural consequences of disease-associated mutations. Human Mutation. 27, 21-30. Pubmed 16281287

Gilbert, H. E., Aslam, M., Guthridge, J. M., Holers, V. M. & Perkins, S. J. (2006). Extended linkers between CR2 SCR-1 and SCR-2 and the Fc fragment in CR2-Ig by X-ray and neutron scattering, analytical ultracentrifugation and constrained molecular modelling. J. Mol. Biol. 356, 397-412. Pubmed 16375923

Almogren, A., Furtado, P. B., Sun, Z., Perkins, S. J. & Kerr, M. A. (2006). Purification, properties and extended solution structure of the complex formed between human immunoglobulin A1 and human serum albumin by scattering and ultracentrifugation. J. Mol. Biol. 356, 413-431. Pubmed 16376934

Jozsi, M., Heinen, S., Hartmann, A., Ostrowicz, C., Hälbich, S., Richter, H., Kunert, A., Licht, C., Saunders, R. E., Perkins, S. J., Zipfel, P. F. & Skerka, C. (2006). C-terminal Mutations of the Immune Regulator Factor H as Described in Patients with Atypical Haemolytic Uraemic Syndrome Show Reduced Ligand Interaction and Endothelial Cell Binding. J. Amer. Soc. Nephrol. 17, 170-177. Pubmed 16338962

Saunders, R. E. & Perkins, S. J. (2006) A user’s guide to the interactive web database of Factor H-Associated Haemolytic Uraemic Syndrome. Seminars in Thromb. Haemost. Pp 160-167. Pubmed 16575691

Licht, C., Heinen, S., Jozsi, M., Löschmann, I., Saunders, R. E., Perkins, S. J., Beck, B., Skerka, C., Kirschfink, M., Hoppe, B. & Zipfel, P. F. (2006) Deletion of a single amino acid (K224) in the regulatory domain of the immune inhibitor factor H reveals a novel pathomechanism for membranoproliferative glomerulonephritis Type II. Kidney Int. 70, 42-50. Pubmed 16612335

Clark, S.J., Higman, V.A., Mulloy, B., Perkins, S.J., Lea, S.M., Sim, R. B., Day, A. J. (2006) H384 allotypic variant of factor H associated with age-related macular degeneration has different heparin-binding properties from the non-disease associated form. J. Biol. Chem. 281, 24713-24720. Pubmed 16787919

Moores, C. A., Perderiset, M., Kappeler, C., Kain, S., Drummond, D., Perkins, S. J., Chelly, J., Cross, R., Houdusse, A. and Francis, F. (2006). Distinct roles of doublecortin modulating the microtubule cytoskeleton. EMBO J., 25, 4448-4457. Pubmed: 16957770

Gilbert, H. E., Asokan, R., Holers, V. M. & Perkins, S. J. (2006) The flexible 15 SCR extracellular domains of human complement receptor type 2 can mediate multiple ligand and antigen interactions. J. Mol. Biol. 362, 1132-1147. Pubmed 16950392

2005

O'Connell, N. M., Saunders, R. E., Lee, C. A., Perry, D. J. and Perkins, S. J. (2005) Structural interpretation of 42 mutations causing factor XI deficiency using homology modeling. J. Thromb. Haemost. 3, 127-138. Pubmed: 15634276

Gilbert, H. E., Eaton, J. T., Hannan, J. P., Holers, V. M., and Perkins, S. J. (2005) Solution structure of the complex between CR2 SCR 1-2 and C3d of human complement: an X-ray scattering and sedimentation modelling study. J. Mol. Biol. 346, 859-873. Pubmed: 15713468

von Ossowski, I., Eaton, J. T., Czjzek, M., Perkins, S. J., Frandsen, T. P., Schülein, M., Panine, P., Henrissat, B. and Receveur-Bréchot, V. (2005) Protein disorder: conformational distribution of the flexible linker in a chimeric double cellulase. Biophys. J. 88, 2823-2832. Pubmed: 15653742

Hu, Y., Sun, Z., Eaton, J. T., Bouloux, P. M. G. & Perkins, S. J. (2005) Extended domain solution structure of the extracellular matrix protein anosmin-1 by X-ray scattering, analytical ultracentrifugation and constrained modeling. J. Mol. Biol. 350, 553-570. Pubmed 15949815

Perkins, S. J. & Furtado, P. B. (2005) Invited review. Complement and immunoglobulin protein structures by X-ray and neutron solution scattering and analytical ultracentrifugation. In “Structural Biology of the Complement System” (Eds: Dimitrios Morikis and John D. Lambris), Chapter 13, pp 293-315. Publisher: Taylor and Francis, Boca Raton, USA. ISBN: 0824725409.

Saunders, R. E., O’Connell, N. M., Lee, C. A., Perry, D. J. & Perkins, S. J. (2005) The factor XI deficiency database: an interactive web database of mutations, phenotypes and structural analysis tools. Human Mutation, 26, 192-198. Pubmed: 16086308

Perkins, S. J., Gilbert, H. E., Lee, Y. C., Sun, Z. & Furtado, P. B. (2005) Invited review. Relating small angle scattering and analytical ultracentrifugation in multidomain proteins. In "Modern Analytical Ultracentrifugation: Techniques and Methods" eds. Scott, D. J., Harding S. E. & Rowe A. J. Royal Society of Chemistry, London, UK. Chapter 15, pp 291-319. ISBN: 0854045473.

Sun, Z., Almogren, A., Furtado, P. B., Chowdhury, B., Kerr, M. A. & Perkins, S. J. (2005). Semi-extended solution structure of human myeloma immunoglobulin D determined by constrained X-ray scattering. J. Mol. Biol. 353, 155-173. Pubmed: 16157351

2004

Furtado, P. B., Whitty, P.W., Robertson, A., Eaton, J. T., Almogren, A., Kerr, M.A., Woof, J.M. & Perkins, S. J. (2004) Solution structure determination of human IgA2 by X-ray and neutron scattering and analytical ultracentrifugation and constrained modelling: a comparison with human IgA1. J. Mol. Biol. 338, 921-941. Pubmed: 15111057

Plevin, M. J., Magalhaes, B. S., Sankar, A., Perkins, S. J. & Driscoll, P. C. (2004) Characterisation and manipulation of a monomer-dimer equilibrium in Pseudomonas aeruginosa dimethylarginine dimethylaminohydrolase. J. Mol. Biol. 341, 171-184. Pubmed: 15312771

Sun, Z., Reid, K. B. M. and Perkins, S. J. (2004) The multidomain solution structures of the complement protein properdin dimer and trimer by X-ray scattering, analytical ultracentrifugation and constrained modeling. J. Mol. Biol. 343, 1327-1343. Pubmed 15491616

Deam, S., Uprichard, J., Eaton, J. T., Perkins, S. J., Dasani, H., Hanley, J. and Dolan, G. (2004) Two new factor X mutations (Pro382Leu and Phe356Cys) associated with low activity and antigen levels. Thromb. Haemost. 92, 1161-1163. Pubmed: 15543350

2003

Deam, S., Uprichard, J. Eaton, J. T., Perkins, S. J. & Dolan, G. (2003) Factor X Leicester: Ile411Phe associated with a low antigen level and a disproportionately low functional activity of Factor X. Journal of Thrombosis & Haemostasis, 1, 603-605. Pubmed: 12871478

Aslam, M., Guthridge, J. M., Hack, B. K., Quigg, R. J., Holers, V. M. & Perkins, S. J. (2003) The extended multidomain solution structures of the complement protein Crry and its chimaeric conjugate Crry-Ig by scattering, analytical ultracentrifugation and constrained modelling: implications for function and therapy. J. Mol. Biol. 329, 525 – 550. Pubmed: 12767833

Idowu, S. M., Gautel, M, Perkins, S. J. & Pfuhl, M. (2003) Structure, stability and dynamics of the central domain of cardiac myosin binding protein C: Implications for multidomain assembly and cardiomyopathy related mutations. J. Mol. Biol. 329, 745-761. Pubmed: 12787675

Lee, Y.-C., Flora, R., McCafferty, J., Gor, J., Tsaneva, I. R. & Perkins, S. J. (2003) A tetramer-octamer equilibrium in Mycobacterium leprae and Escherichia coli RuvA by analytical ultracentrifugation. J. Mol. Biol. 333, 677-682. Pubmed: 14568529

2002

Perkins, S. J. & Goodship, T. H. J. (2002) Molecular modelling of mutations in the C-terminal domains of factor H of human complement: a correlation between haemolytic uraemic syndrome and a predicted heparin binding site. J. Mol. Biol. 316, 217-224. Pubmed 11851332

Peyvandi, F., Menegatti, M., Santagostino, E., Akhavan, S., Uprichard, J., Perry, D. J., Perkins, S. J. & Mannucci, P. M. (2002) Gene mutations and three-dimensional structural analysis in 13 families with Factor X deficiency. Brit. J. Haematology. 117, 685-692. Pubmed: 12028042

Devalia, V., Carter, K., Walker, A. P., Perkins, S. J., Worwood, M., May, A. and Dooley, J. S. (2002) Autosomal dominant reticulo-endothelial iron overload associated with a three base pair deletion in the ferroportin 1 gene (SLC11A3). Blood. 100, 695-697. Pubmed: 12091367

Aslam, M. & Perkins, S. J. (2002). Folded-back solution structure of monomeric Factor H of human complement by scattering, ultracentrifugation and modelling. Fibre Diffraction Review, 10, 72-77.

Lee, Y.-C., Boehm, M. K., Chester, K. A., Begent, R.H.J. & Perkins, S. J. (2002) Reversible dimer formation and stability of the anti-tumour single-chain Fv antibody MFE-23 by neutron scattering, analytical ultracentrifugation, and NMR and FT-IR spectroscopy. J. Mol. Biol. 320, 107-127. Pubmed: 12079338

Hannan, J., Young, K., Szakonyi, G., Overduin, M. J., Perkins, S. J., Chen, X. & Holers, V. M. (2002) Structure of CR2 and CR2/C3d complexes. Biochem. Soc. Transact. 30, 983-989. Pubmed: 12440958

Perkins, S. J., Gilbert, H. E., Aslam, M., Hannan, J. P., Holers, V. M. and Goodship, T. H. J. (2002) Solution structures of complement components by X-ray and neutron scattering and analytical ultracentrifugation. Biochem. Soc. Transact. 30, 996-1001. Pubmed: 12440960

2001

Peyvandi, F., Carew, J. A., Perry, D. J., Hunault, M., Khanduri, U., Perkins, S. J., Mannucci, P. M. & Bauer, K. A. (2001) Abnormal secretion and function of recombinant human factor VII as the result of modification to a calcium binding site caused by a 15 base pair insertion in the factor VII gene. Blood, 97, 960-965. Pubmed: 11159523

Guthridge, J. M., Rakstang, J. K., Young, K. A., Hinshelwood, J., Aslam, M., Robertson, A., Gipson, M. G., Sarrias, M.-R., Moore, W. T., Meagher, M., Karp, D., Lambris, J. D., Perkins, S. J. & Holers, V. M. (2001) Structural studies in solution of the recombinant N-terminal pair of short consensus/complement repeat domains of complement receptor type 2 (CR2/CD21) and its interaction with its ligand C3dg. Biochemistry, 40, 5931-5941. Pubmed: 11352728

Aslam, M. & Perkins, S. J. (2001) Folded-back solution structure of monomeric Factor H of human complement by synchrotron X-ray and neutron scattering, analytical ultracentrifugation and constrained molecular modelling. J. Mol. Biol. 309, 1117-1138. Pubmed: 11399083

Perkins, S. J. (2001) Invited review. Applications of highly constrained molecular modelling scattering curve fits to biologically important proteins. Fibre Diffraction Review, 9, 51-58. (ISSN 1463-8401).

Robertson, A., MacColl, G. S., Nash, J. A. B., Boehm, M. K., Perkins, S. J. & Bouloux, P.-M. G. (2001) Molecular modelling and experimental studies of mutation and cell adhesion sites in the fibronectin type III and whey acidic protein domains of human anosmin-1. Biochem. J. 357, 647-659. Pubmed: 11463336

Perkins, S. J. (2001) Invited review. X-ray and neutron scattering analyses of hydration shells: a molecular interpretation based on sequence predictions and modelling fits. Biophys. Chem. 93, 129-139. Pubmed: 11804721

Guthridge, J. M., Young, K., Gipson, M. G., Sarrias, M.-R., Szakonyi, G., Chen, X. S., Malaspina, A., Donoghue, E., James, J. A., Lambris, J. D., Moir, S. A., Perkins, S. J. and Holers, V. M. (2001) Epitope mapping using the X-ray crystallographic structure of complement receptor type 2 (CR2/CD21): Identification of a highly inhibitory monoclonal antibody that directly recognizes the CR2-C3d interface. J. Immunology, 167, 5758-5766. Pubmed: 11698449

2000

Boehm, M. K., Corper, A. L., Wan, T., Sohi, M.K., Sutton, B. J., Thornton, J. D., Keep, P.A., Chester, K. A., Begent, R. H. J. & Perkins, S. J. (2000) Crystal structure of the anti-(carcinoembryonic antigen) single-chain Fv antibody MFE-23 and a model for antigen binding based on intermolecular contacts. Biochem. J. 346, 519-528. Pubmed: 10677374

Hinshelwood, J. & Perkins, S. J. (2000) Metal-dependent conformational changes in a recombinant vWF-A domain from human factor B: A solution study by circular dichroism, Fourier transform infrared and 1H NMR spectroscopy. J. Mol. Biol. 298, 135-147. Pubmed 10756110

Boehm, M. K. & Perkins, S. J. (2000) Structural models for carcinoembryonic antigen and its complex with the single-chain Fv antibody molecule MFE23. FEBS Letters 475, 11-16. Pubmed 10854848

Peyvandi, F., Jenkins, P. V., Mannucci, P. M., Billio, A., Zeinali, S., Perkins, S. J. & Perry, D. J. (2000) Molecular characterisation and three-dimensional structural analysis of mutations in 21 unrelated families with inherited Factor VII deficiency. Thromb. Haemost. 84, 250-257. Pubmed: 10959697

Hinshelwood, J. & Perkins, S. J. (2000) Conformational changes during the assembly of factor B from its domains by 1H NMR and molecular modelling: their relevance to the regulation of factor B activity. J. Mol. Biol. 301, 1267-1285. Pubmed: 10966820

Perkins, S. J. (2000) Invited review. High-flux X-ray and neutron scattering studies. Chapter 9, Protein-Ligand Interactions: A Practical Approach. Volume 1. Pages 223-262. Editors B. Chowdhry and S. E. Harding (Oxford University Press).

Akhavan, S., Mannucci, P. M., Lak, M., Mancuso, G., Mazzucconi, G., Rocino, A., Jenkins, P. V. & Perkins, S. J. (2000) Identification and three-dimensional structural analysis of nine novel mutations in patients with prothrombin deficiency. Thromb. Haemost. 84, 989-997. Pubmed: 11154146

Nitu-Whalley, I. C., Riddell, A., Lee, C. A., Pasi, K. J., Owens, D., Enayat, M. S., Perkins, S. J. & Jenkins, P. V. (2000) Identification of type 2 von Willebrand Disease in previously diagnosed type 1 patients: a reappraisal using phenotypes, genotypes and molecular modelling. Thromb. Haemost. 84, 998-1004. Pubmed: 11154147

1999

exhibit a different arrangement from that in IgG: a study by X-ray and neutron solution scattering and homology modelling. J. Mol. Biol. 286, 1421-1447. Pubmed: 10064707

Boehm, M. K., Woof, J. M., Kerr, M. A. & Perkins, S. J. (1999) The Fab and Fc fragments of IgA1

Williams, S. C., Hinshelwood, J., Perkins, S. J. & Sim, R. B. (1999) Production and functional activity of a recombinant von Willebrand Factor (vWF-A) domain from human complement factor B. Biochem. J. 342, 625-632. Pubmed: 10477273

Perkins, S. J., Hinshelwood, J., Edwards, Y. J. K. & Jenkins, P.V. (1999) Invited review. Structural and functional modelling of vWF-A domains in complement and coagulation. Biochem. Soc. Transact. 27, 815-821. Pubmed 10830108

Hinshelwood, J., Spencer, D. I. R., Edwards, Y. J. K. & Perkins, S. J. (1999) Identification of the C3b binding site in a recombinant vWF-A domain of complement factor B by surface enhanced laser desorption ionisation affinity mass spectrometry and homology modelling: implications for the activation of factor B. J. Mol. Biol. 294, 587-599. Pubmed: 10610782

1998

Brissett, N. C. & Perkins, S. J. (1998) Conserved basic residues in the C-type lectin and short complement repeat domains of the G3 region of proteoglycans. Biochem. J. 329, 415-424. Pubmed: 9425127

Perkins, S. J., Ashton, A. W., Boehm, M. K. & Chamberlain, D. (1998) Invited review. Molecular structures from low angle X-ray and neutron scattering studies. Int. J. Biolog. Macromol. 22, 1-16. Pubmed: 9513811

Jenkins, P. V., Pasi, K. J. & Perkins, S. J. (1998) Molecular modelling of ligand and mutation sites of the Type A domains of human von Willebrand factor and their relevance to von Willebrand’s disease. Blood, 91, 2032-2044. Won the Angelo Bianchi Bonomi Prize. Pubmed: 9490688

Ashton, A. W., Boehm, M. K., Johnson, D. J. D., Kemball-Cook, G. & Perkins, S. J. (1998) The solution structure of human coagulation factor VIIa in its complex with tissue factor is similar to free factor VIIa: a study of a heterodimeric receptor/ligand complex by X-ray and neutron scattering and computational modelling. Biochemistry, 37, 8208-8217. Pubmed: 9609717

Perkins, S. J., Ullman, C. G., Brissett, N. C., Chamberlain, D. & Boehm, M. K. (1998) Invited review. Analogy and solution scattering modelling: new structural strategies for the multidomain proteins of complement, cartilage and the immunoglobulin superfamily. Immunol. Reviews, 163, 237-250. Pubmed: 9700514

Ullman, C. G., Chamberlain, D., Ansari, A., Emery, V. C., Haris, P. I., Sim, R. B. & Perkins, S. J. (1998) Human complement factor I: its expression by insect cells and its biochemical and structural characterisation. Molecular Immunology, 35, 503-512. Pubmed: 9809578

Chamberlain, D., Ullman, C. G. & Perkins, S. J. (1998) Possible arrangement of the five domains in human complement factor I as determined by a combination of X-ray and neutron scattering and homology modelling. Biochemistry, 37, 13918-13929. Pubmed: 9760226

Chamberlain, D., Keeley, A., Aslam, M., Arenas-Licea, J., Brown, T., Tsaneva, I. R. & Perkins, S. J. (1998) A synthetic Holliday junction is sandwiched between two tetrameric Mycobacterium leprae RuvA structures in solution: new insights from neutron scattering contrast variation and modelling. J. Mol. Biol. 284, 385-400. Pubmed: 9813125

1997

Wallace, E. M., Perkins, S. J., Sim, R. B., Willis, A. C., Feighery, C. & Jackson, J. (1997) C1-inhibitor degradation by plasmin: implications for the control of inflammatory processes. Molecular Medicine, 3, 385-396. Pubmed: 9234243

Chamberlain, D., O’Hara, B. P., Wilson, S. A., Pearl, L. H. & Perkins, S. J. (1997) Oligomerisation of the amide sensor protein AmiC by X-ray and neutron scattering and molecular modelling. Biochemistry, 36, 8020-8029. Pubmed: 9201949

Ullman, C. G. & Perkins, S. J. (1997) The factor I and follistatin domain families: the return of a prodigal son. Biochem. J. 326, 939-941. Pubmed: 9334166

Ashton, A. W., Boehm, M. K., Gallimore, J. R., Pepys, M. B. & Perkins, S. J. (1997) Pentameric and decameric structures in solution of the serum amyloid P component by X-ray and neutron scattering and molecular modelling analyses. J. Mol. Biol. 272, 408-422. Pubmed: 9609717

1996

Brissett, N. C. & Perkins, S. J. (1996) Molecular modelling analyses of the C-type lectin domain in human aggrecan. Biochem. Soc. Transact. 24, 99S-99S. Pubmed: 8674785

Boehm, M. K., Mayans, M. O., Thornton, J. D., Begent, R. H. J., Keep, P. A. & Perkins, S. J. (1996) Extended glycoprotein structure of the seven domains in human carcinoembryonic antigen by X-ray and neutron solution scattering and an automated curve fitting procedure: implications for cellular adhesion. J. Mol. Biol. 259, 718-736. Pubmed: 8683578

Brissett, N. C. & Perkins, S. J. (1996) The protein fold of the hyaluronate-binding proteoglycan tandem repeat domain of link protein, aggrecan and CD44 is similar to that of the C-type lectin superfamily. FEBS Lett. 388, 211-216. Front cover of journal. Pubmed: 8690089

Edwards, Y. J. K. & Perkins, S. J. (1996) Assessment of protein fold predictions from sequence information: the predicted α/β doubly wound fold of the von Willebrand Factor Type A domain is similar to its crystal structure. J. Mol. Biol. 260, 277-285. Pubmed: 8764406

Meyer, D. F., Nealis, A. S., MacPhee, C. H., Groot, P. H. E., Suckling, K. E., Bruckdorfer, K. R. & Perkins, S. J. (1996) Time-course studies by synchrotron X-ray solution scattering of the structure of human low-density lipoprotein during Cu2+-induced oxidation in relation to changes in lipid composition. Biochem. J. 319, 217-227. Pubmed: 8870672

Ullman, C. G., Haris, P. I., Galloway, D., Emery, V. C. & Perkins, S. J. (1996) The zinc-binding motifs of human papillomavirus E7 and E6 proteins exhibit distinct α/β secondary structures: a study by consensus sequence averaging and spectroscopy of E7. Biochem. J. 319, 229-239. Pubmed: 8870673