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GeneTxNeuro Costs and Ordering Information

Information on the service costs of the GeneTxNeuro Facility and what you will need to provide to use the facility.

Ordering information

What you would need to provide

pAAV-ITR-GOI-ITR DNA prep:

  • Size ≈ 4.7kb
  • Endonuclease & recombination deficient competent cells (SURE, Stbl3)
  • Endotoxin-free maxi prep
  • Control cut with SmaI restriction enzyme
  • 200ug DNA for small scale prep, 500ug for large scale prep

AAV capsid DNA prep:

  • Endonuclease & recombination deficient competent cells (SURE, Stbl3)
  • Endotoxin-free maxi prep
  • Control cut with restriction enzymes
  • 200ug DNA for small scale prep, 500ug for large scale prep

pLV-LTR-GOI-LTR DNA prep:

  • Size ≈ 8kb
  • 100ug DNA

Services

Consultation on vector design


Two production scales

  • Small scale (½ prep): 500ul
  • Large scale (full prep): 1ml

Expected yield*

  • AAV:  1ml of ≥ 1x1013 vg/ml
  • Lentivirus: 1ml of ≈108 TU/ml

*Some transgenes can be cytotoxic, and occasionally different capsids can produce less efficiently. This can result in lower titer.

Cost

AAV

  • Small scale: £1,600
  • Large scale: £2,000

Lentivirus

  • Small scale: £600
  • Large scale: £1,000

Users can decide to pay 10% extra charge as insurance for failed preps. This will allow users to run an extra production process without additional costs. 

QC provided

  • Vector genome concentration (qPCR)
  • Vector purity (SDS gel)
  • Endotoxin levels (LAL test)

Email Giulia to enquire about use of services