High-throughput screening using classical and chemical genetic approaches has emerged as a powerful tool to identify cellular pathways, potential therapeutic targets and therapeutic lead compounds. For a long time disregarded as “fishing expeditions”, it is now accepted that functional genomic screening is the way forward for making unexpected, ground-breaking discoveries in biomedical research.
In fact, much of what we know about how cells function has come from unbiased screening approaches. This paradigm shift has been facilitated by the availability of complete genome sequences and the advent of gene-specific knockdown technologies using RNA Interference (RNAi) and CRISPR/Cas9 genome editing.
The High-Content Biology Laboratory uses high-throughput screening techniques to facilitate translational research. A main emphasis is the use of high-content screening techniques. In addition, our instrumentation allows analysis of various other assay systems including confocal microscopy, fluorescence, absorbance, luminescence and flow cytometry. We are using small molecule, cDNA, siRNA and CRISPR/Cas9 screening libraries.
We provide a high-throughput screening platform for projects that involve biosafety level 3 pathogens such as human Immunodeficiency Virus or Hepatitis Virus. This platform is located at our sister site at the Wohl Virion Centre, University College London, and uses a unique setup of liquid handling robots (CyBio WellVario, CyBi Drop) in a biosafety cabinet that enables fully automated processing of infectious samples in a 384-well format.
In addition, we work closely with the MRC Dementia Platform UK and have integrated specialized equipment for the use of induced pluripotent derived stem cells and primary neurons in high-content screening.
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The high-content biology platform is located in the UCL Medical Sciences/Anatomy building and invites projects from all faculties at UCL. If you are interested in screening, using equipment or just for advice and expertise, please contact Robin Ketteler.
Daniel Cutler (LMCB, UK)
Rob De Bruin (LMCB, UK)
Janos Kriston-Vizi (LMCB, UK)
Alison Lloyd (LMCB, UK)
Mark Marsh (LMCB, UK)
Sara Mole (LMCB, UK)
Franck Pichaud (LMCB, UK)
Chris Stefan (LMCB, UK)
Daniel Hochhauser (UCL Cancer Institute, UK)
Paolo Salomoni (UCL Cancer Institute, UK)
Tom Warner (UCL Institute of Neurology, UK)
Helene Plun-Favreau (UCL Institute of Neurology, UK)
Sarah Tabrizi (UCL Institute of Neurology, UK)
Aroon Hingorani (UCL Farr Institute, UK)
JP Romero (UCL Farr Institute, UK)
Paul Whiting (ARUK DDI, UK)
Ariberto Fassati (WIBR, UK)
Dagan Jenkins (UCL ICH, UK)
Francesco Muntoni (UCL ICH, UK)
Manju Kurian (UCL ICH, UK)
Paul Gissen (UCL ICH, UK)
Anisur Rahman (UCL Medicine)
Vania Braga (Imperial College London, UK)
Gabor Foldes (Imperial College London, UK)
Richard Angell (UCL School of Pharmacy, UK)
Gyuri Szabadkai (UCL CDB, UK)
Buzz Baum (MRC LMB, UK)
Jason Mercer (Birmingham University, UK)
Ricardo Henriques (IGC, Portugal)