Electron microscopy (EM) provides a unique view of biological systems, allowing the visualization of the ultrastructure of your sample with nm resolution. It has provided the basis of our understanding of tissue and cellular architecture and continues to reveal a wealth of information about how cells and tissues function in health and disease.
The LMCB has been specialising in cell biological EM for over 20 years. The facility comprises state-of-the-art specimen preparation and imaging technologies which, together with two experienced researchers, support the needs of the LMCB groups in all their EM research.
With experience of a wide range of electron microscopy techniques as well as a broad range of model organisms, we can provide advice, training and practical support for researchers throughout the entire timeline of their projects. For example: planning experiments, sample preparation, imaging and data analysis. The support given is tailored to each project, and can vary from advice and technical support for the more experienced, full training in required techniques and the equipment use for the less experienced researchers or the undertaking of the EM projects ourselves, a collaborative endeavour as part of the ongoing research.
Roubinet C, et al (2021). Asymmetric nuclear division in neural stem cells generates sibling nuclei that differ in size, envelope composition, and chromatin organization. Curr Biol. Sep 27;31(18):3973-3983
Lopes da Silva M, et al (2019). A GBF1-Dependent Mechanism for Environmentally Responsive Regulation of ER-Golgi Transport. Dev Cell. 2019 Jun 3;49(5):786-801.e6. doi: 10.1016/j.devcel.2019.04.006.
Russell MR, et al (2017). 3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy. J Cell Sci. Jul 21. pii: jcs.188433
Lopes da Silva M, et al (2016). Type II PI4-kinases control Weibel-Palade body biogenesis and von Willebrand factor string formation in Human endothelial cells. J. Cell Sci. May 15;129(10):2096-105.
Cattin AL, et al (2015). Macrophage-induced blood vessels guide Schwann cell collective migration during peripheral nerve regeneration. Cell Aug 27;162(5):1127-39
Jemima Burden (Technology leader)
Ian White (Deputy electron microscopy leader)
The EM equipment are principally used by LMCB staff but access is open to all UCL staff as well as to external users on a collaboration basis. Please contact Jemima Burden for details.
Sophie Acton (LMCB, UK)
Daniel Cutler (LMCB, UK)
Rob De Bruin (LMCB, UK)
Robin Ketteler (LMCB, UK)
Janos Kristin-Vizi (LMCB, UK)
Alison Lloyd (LMCB, UK)
Yanlan Mao (LMCB, UK)
Mark Marsh (LMCB, UK)
Chris Stefan (LMCB, UK)
Frances Brodsky (UCL, UK)
Lucy Collinson (FCI, UK)
Derek Hausenluoy (UCL, UK)
Josef Kittler (UCL, UK)
Paul Gissen (ICH, UCL, UK)
Tom Nightingale (QMUL, UK)
Patricia Salinas (UCL, UK)
Buzz Baum (MRC LMB, UK)
Jason Mercer (Birmingham University, UK)
Ewa Paluch (Cambridge University, UK)
Kevin Staras (University of Sussex, UK)