Publications

2014

Massie I, Levis HJ, Daniels JT (2014) Response of human limbal epithelial cells to wounding on 3D RAFT tissue equivalents: Effect of airlifting and human limbal fibroblasts. Exp Eye Res 127: 196-205

Kureshi AK, Drake RA, Daniels JT (2014) Challenges in the development of a reference standard and potency assay for the clinical production of RAFT tissue equivalents for the cornea. Regen Med 9(2):167-77.

Dziasko MA, Armer HE, Levis HJ, Shortt AJ, Tuft S, Daniels JT. (2014) Localisation of epithelial cells capable of holoclone formation in vitro and direct interaction with stromal cells in the native human limbal crypt. PLoS One 9(4):e94283. 

Shortt AJ, Bunce C, Levis HJ, Blows P, Dore CJ, Vernon A, Secker GA, Tuft SJ, Daniles JT. (2014) Three-year outcomes of cultured gimbal epithelial allografts in aniridia and Stevens-Johnson syndrome evaluated using the Clinical Outcome Assessment in Surgical Trials assessment tool. Stem Cells Transl Med. 3 (2):265-75. 

2013

Levis HJ, Massie I, Dziasko MA, Kaasi A, Daniels JT. (2013) Rapid tissue engineering of biomimetic human corneal limbal crypts with 3D niche architecture. Biomaterials 34 (35):8860-8. 

Angunawela RI, Mehta JS, Daniels JT. (2013) Ex-vivo ocular surface stem cell therapies: current techniques, applications, hurdles and future directions. Expert Rev Mol Med 15: e4

2012

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Levis HJ, Peh GS, Toh KP, Poh R, Shortt AJ, Drake RA, Mehta JS, Daniels JT (2012) Plastic compressed collagen as a novel carrier for expanded human corneal endothelial cells for transplantation. PLoS One. 7(11):e50993.

Levis HJ, Menzel-Severing J, Drake RA, Daniels JT. (2012) Plastic compressed collagen constructs for ocular cell culture and transplantation: A new and improved method of confined fluid loss. Curr Eye Res 38(1):41-52. 

Schrader S, O'Callaghan AR, Tuft SJ, Beaconsfield M, Geerling G, Daniels JT. (2012) Wnt signalling in an in vitro niche model for conjunctival progenitor cells. J Tissue Eng Regen Med. 2012 Aug 30.

Schrader S, Tuft SJ, Beaconsfield M, Borrelli M, Geerling G, Daniels JT (2012) Evaluation of Human MRC-5 Cells as a Feeder Layer in a Xenobiotic Free Culture System for Conjunctival Epithelial Progenitor Cells. Curr Eye Res 37(12):1067-74 

Notara M, Hernandez D, Mason C, Daniels JT (2012) Characterization of the phenotype and functionality of corneal epithelial cells derived from mouse embryonic stem cells. Regen Med 7(2):167-78

Notara M,Shortt AJ,O'Callaghan AR,Daniels JT (2012). The impact of age on the physical and cellular properties of the human limbal stem cell niche. Age 35(2):289-300.

2011

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Shortt AJ,Tuft SJ,Daniels JT (2011). Corneal stem cells in the eye clinic. Br Med Bull 100, 209-225. 

O'Callaghan AR, Daniels JT, Mason C (2011) Effect of sub-atmospheric oxygen on the culture of rabbit limbal epithelial cells. Curr Eye Res 36(8):691-8.

Merjava S,Brejchova K,Vernon A,Daniels JT,Jirsova K (2011). Cytokeratin 8 Is Expressed in Human Corneoconjunctival Epithelium, Particularly in Limbal Epithelial Cells. Invest Ophthalmol Vis Sci 52(2), 787-794.

Saw V,Offiah I,Schmidt E,Galatowicz G,Zillikens Z,Dart J,Daniels J,Calder V (2011). Profibrotic Phenotype of Conjunctival Fibroblasts from Mucous Membrane Pemphigoid. Am J Pathol 178(1), 187-197.

O'Callaghan AR,Daniels JT (2011). Concise review: limbal epithelial stem cell therapy: controversies and challenges. Stem Cells 19(12), 1923-1932.

Notara M,Schrader S,Daniels JT (2011). The porcine limbal epithelial stem cell niche as a new model for the study of transplanted tissue-engineered human limbal epithelial cells. Tissue Eng Part A 17(5-6).
 

2010

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Notara M,Daniels JT (2010). Characterisation and functional features of a spontaneously immortalised human corneal epithelial cell line with progenitor-like characteristics. Brain Res Bull 81(2-3), 279-286.

Notara M,Shortt AJ,Galatowicz G,Calder V,Daniels JT (2010). IL6 and the human limbal stem cell niche: a mediator of epithelial-stromal interaction. Stem Cell Res 5(3), 188-200.

Watson SL,Daniels JT,Geerling G,Dart JK (2010). Clinical trials of therapeutic ocular surface medium for moderate to severe dry eye. Cornea 29(11), 1241-1246.

Levis HJ,Brown RA,Daniels JT (2010). Plastic compressed collagen as a biomimetic substrate for human limbal epithelial cell culture. Biomaterials 31(30), 7726-7737.

Ahmad S,Kolli S,Lako M,Figueiredo F,Daniels JT (2010). Stem cell therapies for ocular surface disease. Drug Discov Today 15(7-8), 306-313.

Shortt AJ,Tuft SJ,Daniels JT (2010). Ex vivo cultured limbal epithelial transplantation. A clinical perspective. Ocul Surf 8(2), 80-90.

Notara M,Alatza A,Gilfillan J,Harris AR,Levis HJ,Schrader S,Vernon A,Daniels JT (2010). In sickness and in health: Corneal epithelial stem cell biology, pathology and therapy. Exp Eye Res 90(2), 188-195.

Schrader S,Notara M,Tuft SJ,Beaconsfield M,Geerling G,Daniels JT (2010). Simulation of an in vitro niche environment that preserves conjunctival progenitor cells. Regen Med 5(6), 877-889.

2009

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Schrader S,Notara M,Beaconsfield M,Tuft SJ,Daniels JT,Geerling G (2009).Tissue engineering for conjunctival reconstruction: established methods and future outlooks. Curr Eye Res 34(11), 913-924.

Saw VP,Offiah I,Dart RJ,Galatowicz G,Dart JK,Daniels JT,Calder VL (2009). Conjunctival interleukin-13 expression in mucous membrane pemphigoid and functional effects of interleukin-13 on conjunctival fibroblasts in vitro. Am J Pathol 175(6), 2406-2415.

Levis H, Daniels JT (2009). New technologies in limbal epithelial stem cell transplantation. Curr Opin Biotechnol 20(5), 593-7.

Schrader S, Notara M, Beaconsfield M, Tuft S, Geerling G, Daniels JT. (2009). Conjunctival epithelial cells maintain stem cell properties after long-term culture and cryopreservation. Regen Med 4(5), 677-87.

Deshpande,P., Notara,M., Bullett,N.A., Daniels,J.T., Haddow,D.B., Mac, Neil S. (2009). Development of a surface modified contact lens for transfer of cultured limbal epithelial cells for ocular surface diseases. Tissue Eng Part A 15 (10), 2889-902.

Saw,V.P., Dart,R.J., Galatowicz,G., Daniels,J.T., Dart,J., Calder,V.L. (2009). Tumour Necrosis Factor-{alpha} in Ocular Mucous Membrane Pemphigoid and its effect on Conjunctival Fibroblasts. Invest Ophthalmol Vis Sci 50 (11), 5310-7.

Shortt,A.J., Secker,G.A., Lomas,R.J., Wilshaw,S.P., Kearney,J.N., Tuft,S.J., Daniels,J.T. (2009). The effect of amniotic membrane preparation method on its ability to serve as a substrate for the ex-vivo expansion of limbal epithelial cells. Biomaterials 30(6), 1056-65.

2008

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Harris,A.R., Daniels,J.T., Mason,C. (2008). Effect of sub-atmospheric oxygen on the culture of rabbit limbal epithelial stem cells. International Society for Stem Cell Research, Philadelphia

Limb,G.A., Daniels,J.T. (2008). Ocular regeneration by stem cells: present status and future prospects. Br Med Bull 85, 47-61.

Liu,L., Tiffany,J., Dang,Z., Dart,J.K., Watson,S.L., Daniels,J.T., Geerling,G. (2008). Nourish and nuture - development of a nutrient ocular lubricant. Investigative Ophthalmology and Visual Science 50 (6), 2932-9.

Notara,M.,Daniels,J.T. (2008). Biological principals and clinical potentials of limbal epithelial stem cells. Cell Tissue Research 331(1), 135-143.

Secker,G.A., Daniels,J.T. (2008). Corneal epithelial stem cells: Deficiency and regulation. Stem Cell Reviews 4(3), 159-168.

Secker,G.A., Shortt,A.J., Sampson,E., Schwarz,Q.P., Schultz,G.S., Daniels,J.T. (2008). TGFβ-stimulated re-epithelialisation is regulated by CTGF and Ras/MEK/ERK signalling. Experimental Cell Research 314(1), 131-142.

Shortt,A.J., Secker,G.A., Rajan,M., Meligonis,G., Dart,J.K., Tuft,S.J., Daniels,J.T. (2008). Ex vivo expansion and transplantation of limbal epithelial stem cells. Ophthalmology 115(11), 1989-97.

Soares,S., Daniels,J.T. (2008), Crossing Over: Exchanges in Art and Biotechnologies. The Royal Institution of Great Britain.

Soares,S., Daniels,J.T., Harris,A. (2008), BioPlay, The Dana Centre at the Science Museum, London.

Watson,S.L., Secker,G.A., Dart,J.K.G., Daniels,J.T. (2008). The effect of therapeutic human serum drops on corneal stromal wound healing activity. Current Eye Research 33(8), 641-652. 

2007

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Daniels,J. T., Notara,M., Shortt,A. J., Secker,G., Harris,A., Tuft,S. J. (2007). Limbal epithelial stem cell therapy. Expert Opinion on Biological Therapy 7(1), 1-3.

Daniels,J.T. (2007). Stem cells: moving the biology to the clinic. Regenerative Medicine 2(3), 313-315.

Deshpande,P., Bullet,N., Notara,M., Daniels,J.T., Haddow,D., MacNeil,S. (2007). Use of rabbit cornea model for the development of a cell transfer system for limbal epithelial cells. Tissue Engineering, 13 (7), 1773-1773.

Notara,M.,Haddow,D.B.,MacNeil,S.,Daniels,J.T. (2007). A xenobiotic-free culture system for human limbal epithelial stem cells. Regenerative Medicine 2(6), 919-927.

Notara,M., Bullett,N.A., Deshpande,P., Haddow,D.B., MacNeil,S., Daniels,J.T. (2007). Plasma polymer coated surfaces for serum-free culture of limbal epithelium for ocular surface disease. Journal of Materials Science: Materials in Medicine 18(2), 329-338.

Shortt,A.J., Secker,G.A., Munro,P.M., Khaw,P.T., Tuft,S.J., Daniels,J.T. (2007).
Characterization of the limbal epithelial stem cell niche: novel imaging techniques permit in vivo observation and targeted biopsy of limbal epithelial stem cells. Stem Cells 25(6), 1402-1409. 

2006

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Daniels,J.T., Harris,A.R., Mason,C. (2006). Corneal epithelial stem cells in health and disease. Stem Cell Reviews 2(3), 247-254.

Daniels,J.T., Secker,G.A., Shortt,A.J., Tuft,S.J., Seetharaman,S. (2006). Stem cell therapy delivery- treading the regulatory tightrope. Regenerative medicine 1(5), 715-719.

Limb,G.A., Daniels,J.T., Cambrey,A.D., Secker,G.A., Shortt,A.J., Lawrence,J.M., Khaw,P.T. (2006). Current prospects of adult stem cell-based therapies for ocular repair and regeneration. Curr Eye Res. 31(5), 381-390.

2005

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Secker,G., Watson,S.L., Shortt,A.J., Grotendorst,G.R., Schultz,G.S., Khaw,P.T., Daniels,J.T. (2005). Effects of CTGF on HCE re-epithelialisation and migration. Investigative Ophthalmology and Visual Science, 46, 2139.

2004

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Crowston,J.G., Chang,L.H., Daniels,J.T., Khaw,P.T., Akbar,A.N. (2004). T lymphocyte mediated lysis of mitomycin C treated Tenon's capsule fibroblasts. British Journal of Ophthalmology 88(3), 399-405.

Garratt,Q., Khaw,P.T., Blalock,T.D., Schultz,G.S., Grotendorst,G.R., Daniels,J.T. (2004). Involvement of CTGF in TGF-beta 1-stimulation of myofibroblast differentiation and collagen matrix contraction in the presence of mechanical stress. Investigative Ophthalmology and Visual Science 45(4), 1109-1116.

Khaw,P.T., Clarke,J.C.K., Mead,A.L., Wong,T.T.L., Cambrey,A., Daniels,J.T. (2004). Controlling tissue repair and regeneration after surgery: new treatments and techniques. Chapter 22 in Shaarawy,T., Flammer,J. (ed.) Glaucoma therapy current issues and controversies. , 291-309.

Watson,S.L., Secker,G.A., Dart,J.K., Daniels,J.T. (2004). The effect of human serum on human corneal fibroblast wound healing activities. Investigative Ophthalmology and Visual Science, 45, 533.

Wong,T.T., Daniels,J.T., Crowston,J.G., Khaw,P.T. (2004). MMP inhibition prevents human lens epithelial cell migration and contraction of the lens capsule. British Journal of Ophthalmology 88(7), 868-872.

Wong,T.T.L., Daniels,J.T., Murphy,G., Khaw,P.T. (2004). MMP inhibition modulates protease production and matrix contraction by myofibroblasts in mechnically loaded collagen gels. Investigative Ophthalmology and Visual Science, 45, 375.
 

2003

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Daniels,J.T., Cambrey,A.D., Occleston,N.L., Garrett,Q., Tarnuzzer,R.W., Schultz,G.S., Khaw,P.T. (2003). Matrix metalloproteinase inhibition modulates fibroblast-mediated matrix contraction and collagen production in vitro. Investigative Ophthalmology and Visual Science 44(3), 1104-1110.

Daniels,J.T., Garrett,Q., Blalock,T.D., Grotendorst,G.R., Khaw,P.T., Schultz,G.S. (2003). Human corneal epithelial cells proliferate in response to exogenous CTGF and release CTGF during differentiation into multilayered epithelium. Investigative Ophthalmology and Visual Science, 44, 3805

Daniels,J.T., Geerling,G., Alexander,R.A., Murphy,G., Khaw,P.T., Saarialho-Kere,U. (2003). Temporal and spatial expression of matrix metalloproteinases during wound healing of human corneal tissue. Experimental Eye Research 77(6), 653-664.

Daniels,J.T., Limb,G.A., Saarialho-Kere,U., Murphy,G., Khaw,P.T. (2003). Human corneal epithelial cells require MMP-1 for HGF-mediated migration on collagen I. Investigative Ophthalmology and Visual Science 44(3), 1048-1055.

Daniels,J.T., Schultz,G.S., Blalock,T.D., Garrett,Q., Grotendorst,G.R., Dean,N.M., Khaw,P.T. (2003). Mediation of transforming growth factor-beta(1)-stimulated matrix contraction by fibroblasts: a role for connective tissue growth factor in contractile scarring. American Journal of Pathology 163(5), 2043-2052.

Garrett,Q., Khaw,P.T., Blalock,T.D., Schultz,G.S., Grotendorst,G.R., Daniels,J.T. (2003). The effect of connective tissue growth factor on corneal fibroblast-populated collagen lattice contraction in the presence of mechanical stress. Investigative Ophthalmology and Visual Science, 44, 895.

2002

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Crowston,J.G., Chang,L.H., Constable,P.H., Daniels,J.T., Akbar,A.N., Khaw,P.T. (2002). Apoptosis gene expression and death receptor signaling in mitomycin-C-treated human tenon capsule fibroblasts. Investigative Ophthalmology and Visual Science 43(3), 692-699.

Daniels,J.T., Schultz,G.S., Grotendorst,G.R., Dean,N.M>, Khaw,P.T. (2002). Connective tissue growth factor regulates TGF beta1 mediated matrix contraction by fibroblasts. Investigative Ophthalmology and Visual Science, 43, 858.

Khaw,P.T., Cambrey,A.D., Limb,G.A., Daniels,J.T. (2002). Gene therapy: new "magic bullets" to prevent ocular scarring. The British Journal of Ophthalmology 86(5), 490-492.

Limb,G.A., Daniels,J.T., Pleass,R., Charteris,D.G., Luthert,P.J., Khaw,P.T. (2002). Differential expression of matrix metalloproteinases 2 and 9 by glial Muller cells: response to soluble and extracellular matrix-bound tumor necrosis factor-alpha. American Journal of Pathology 160(5), 1847-1855. 

Occleston,N.L., Daniels,J.T., Khaw,P.T. (2002). Wound healing: laboratory investigation and modulating agents. Chapter 7 in Hunt,B.J., Poston,L., Schachter,M., Halliday,A. (ed.) An Introduction to Vascular Biology From Basic Science to Clinical Practice. Cambridge University Press, 2nd edition, 129-165

Wong,T.T., Sethi,C., Daniels,J.T., Limb,G.A., Murphy,G., Khaw,P.T. (2002). Matrix metalloproteinases in disease and repair processes in the anterior segment. Survey of Ophthalmology 47(3), 239-256.

Wong,T.T.L., Daniels,J.T., Mead,A.L., Murphy,G., Khaw,P.T. (2002). Inhibition of matrix metalloproteinases reduces scarring following experimental glaucoma filtration surgery. Investigative Ophthalmology and Visual Science, 43, 870.

2001

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Daniels,J.T., Dart,J.K., Tuft,S.J., Khaw,P.T. (2001). Corneal stem cells in review. Wound Repair and Regeneration 9(6), 483-494.

Daniels,J.T., Tallqvist,A., Reichardt,O., Geerling,G., Alexander,R.A., Murphy,G., Khaw,P.T., Saarialho-Kere,U. (2001). Temporal and spatial expression of matrix metallproteinases during wound healing of human cornea. Investigative Ophthalmology and Visual Science, 42, 5017

Geerling,G., Daniels,J.T., Dart,J.K., Cree,I.A., Khaw,P.T. (2001). Toxicity of natural tear substitutes in a fully defined culture model of human corneal epithelial cells. Investigative Ophthalmology and Visual Science 42(5), 948-956.

Geerling,G., Limb,G.A., Daniels,J.T., Dart,J.K., Khaw,P.T. (2001). Immunolocalisation of matrix metalloproteinases and tissue inhibitors of metalloproteinases in sterile corneal ulcers. Investigative Ophthalmology and Visual Science, 42, 3077.

Khaw,P.T., Chang,L., Wong,T.T., Mead,A., Daniels,J.T., Cordeiro,M.F. (2001). Modulation of wound healing after glaucoma surgery. Current Opinion in Ophthalmology 12(2), 143-148.

Limb,G.A., Daniels,J.T., Pleass,R., Blood,I., Charteris,D.G., Luthert,P.J., Khaw,P.T. (2001). Expression of MMP2 and MMP9 by retinal Muller cells: modulation by extracellular matrix-bound TNF alpha. Investigative Ophthalmology and Visual Science, 42, 1077

Poon,A.C., Geerling,G., Dart,J.K., Fraenkel,G.E., Daniels,J.T. (2001). Autologous serum drops for dry eyes and epithelial defects: clinical and in vitro toxicity studies. British Journal of Ophthalmology 85(10), 1188-1197.

Stephens,P., Davies,K.J., Occleston,N., Pleass,R.D., Kon,C., Daniels,J., Khaw,P.T., Thomas,D.W. (2001). Skin and oral fibroblasts exhibit phenotypic differences in extracellular matrix reorganization and matrix metalloproteinase activity. British Journal of Dermatology 144(2), 229-237.

Wong,T.T.L., Daniels,J.T., Limb,G.A., Murphy,G., Khaw,P.T. (2001). MMP1 and MMP2 are implicated in myofibroblast mediated collagen contraction. Investigative Ophthalmology and Visual Science, 42, 2917.

2000

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Cordeiro,M.F., Chang,L., Lim,K.S., Daniels,J.T., Pleass,R.D., Sirawardena,D., Khaw,P.T. (2000). Modulating conjunctival wound healing. Eye 14(3), 536-547.

Daniels,J.T., Khaw,P.T. (2000). Temporal stimulation of corneal fibroblast wound healing activity by differentiating epithelium in vitro. Investigative Ophthalmology and Visual Science 41(12), 3754-3762.

Daniels,J.T., Murphy,G., Khaw,P.T. (2000). A multi-functional role for MMP-9 in corneal epithelial cells? Investigative Ophthalmology and Visual Science, 41, 4833

Geerling,G., Daniels,J.T., Khaw,P.T., Cree,I.A., Dart,J.K. (2000). Toxicity of serum and saliva on cultured primary corneal epithelium. Investigative Ophthalmology and Visual Science, 41, 1432.

1999

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Crowston,J.G., Akbar,A.N., Constable,P.H., Chang,L., Daniels,J.T., Khaw,P.T. (1999). Fas (CD95) and mitomycin C-induced Tenon's capsule apoptosis. Investigative Ophthalmology and Visual Science, 40, 5101.

Daniels,J.T., Occleston,N.L., Crowston,J.G., Khaw,P.T. (1999). Effects of antimetabolite induced cellular growth arrest on fibroblast-fibroblast interactions. Experimental Eye Research 69(1), 117-127.

Geerling,G., Joussen,A.M., Daniels,J.T., Mulholland,B., Khaw,P.T., Dart,J.K. (1999). Gelatinases in sterile corneal melts. Investigative Ophthalmology and Visual Science, 40, 3259.

Geerling,G., Joussen,A.M., Daniels,J.T., Mulholland,B., Khaw,P.T., Dart,J.K. (1999). Matrix metalloproteinases in sterile corneal melts. Annals of the New York Academy of Sciences 878, 571-574.

1998

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Crowston,J.G., Akbar,A.N., Constable,P.H., Daniels,J.T., Khaw,P.T. (1998). Antimetabolite induced apoptosis in Tenon's capsule fibroblasts. Investigative Ophthalmology and Visual Science 39(2), 449-454.

Daniels,J.T., Occleston,N.L., Crowston,J.G., Cordeiro,M.F., Alexander,R.A., Wilkins,M., Porter,R., Brown,R., Khaw,P.T. (1998). understanding and controlling the scarring response. Microscopy research and Technique 42, 317-333.

Kon,C.H., Occleston,N.L., Charteris,D., Daniels,J., Aylward,G.W., Khaw,P.T. (1998). A prospective study of matrix metalloproteinases in proliferative vitroretinopathy. Investigative Ophthalmology and Visual Science 39(8), 1524-1529.

Occleston,N.L., Daniels,J.T., Tarnuzzer,R.W., Sethi,K.K., Alexander,R.A., Bhattacharya,S.S., Khaw,P.T. (1998). Single exposures to antiproliferatives: long term effects on ocular fibroblast wound healing behaviour. Investigative Ophthalmology and Visual Science 38(10), 1998-2007.

1997

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Daniels,J.T.,Kearney,J.N.,Ingham,E. (1997). An investigation into the potential of extracellular matrix factors for attachment and proliferation of human keratinocytes in skin substitutes. Burns 23(1), 26-31.

1996

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Daniels,J.T., Kearney,J.N., Ingham,E. (1996). Human keratinocyte isolation and cell culture: a survey of current practices in the UK. Burns 22(1), 35-39. 

1995

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Daniels,J.T.,Harris,I.R.,Kearney,J.N.,Ingham,E. (1995). Calcium: a crucial consideration in serum-free keratinocyte culture. Experimental Dermatology 4(4), 183-191.