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Flow Cytometry Translational Technology Platform

The Flow Cytometry Translational Technology Platform provides equipment and expertise in flow analysis, cell sorting and mass cytometry to cancer researchers at UCL.

Flow cytometry is a powerful tool that uses lasers to rapidly quantify the fluorescent and light scattering properties of single cells or particles in suspension. Cell components can be labelled with fluorescent dyes to allow different populations to be delineated from the bulk population. Dyes can be used to label and quantify DNA, epitope expression and also for functional assays including oxidative metabolism, apoptosis/viability, calcium flux and proliferation.

The Flow Cytometry Translational Technology Platform at the UCL Cancer Institute is equipped with the latest cutting-edge instrumentation with a BD Symphony A5 capable of measuring up to 28 fluorescent parameters including access to the latest fluorescent dye technology and a Helios Mass cytometer capable of measuring >35 parameters simultaneously per cell. The facility also has three 4-laser Fortessa analysers and three cell sorters, one of which is housed in a Class II Biosafety cabinet. Services provided by the facility include Cell Sorting, Mass cytometry and analyser training from beginner to advanced levels.

The TTP is supported by two technicians and the facility manager with many years of cumulative experience. We provide expertise in experimental design for both sorting and analysis as well as technical assistance.

Booking

Book Equipment Here

Following appropriate training, new users should contact ci.crukcentre@ucl.ac.uk to register with Clustermarket.

Equipment

Analysers

BD Fortessa X20

We have two Fortessa X20s in the TTP, both instruments are equipped with 4 lasers capable of measuring 16 parameters providing a robust and flexible system for researchers in the CI. Full instrument training is provided to researchers by members of the TTP.

Fortessa

 

BD LSR Fortessa C

The LSR Fortessa C is a 4 laser 16 parameter instrument equipped with Violet, Blue, Yellow-Green and Red lasers similar to the Fortessa X20s also in lab 303. In order to use this instrument you will require a DIVA account to be set up – please ask a member of the TTP team to do this for you.

 

BD FACSymphony

Based off the existing fluorescence technology the Symphony is an advanced analyser equipped with 5 high powered lasers including a 60mW UV laser, advanced electronics and upgraded detector arrays that allow the detection of up to 28 parameters simultaneously.

BD Symphony

 

Helios – Mass Cytometer

The latest CyToF instrument from Fluidigm that is capable of measuring greater than 40 parameters simultaneously.  The instrument combines aspects of traditional flow and mass spectrometry measuring mass instead of fluorescence.

Helios Mass Cytometer
Miltenyi GentleMACS Octo Dissociator

The Miltenyi GentleMACS Octo Dissociator system (with heated cuffs) is a benchtop instrument for the semi-automated and standardized tissue dissociation or homogenization of up to eight samples. Single-cell suspensions or thorough homogenates from virtually any tissues are easily and reproducibly obtained using the unique C Tubes or M Tubes. These single-use gentleMACS Tubes allow sample preparation in a closed and sterile system, providing a high level of user safety and minimize cross-contamination.

Sorters

BD FACS Aria Fusion

Aria Fusion is a 5 laser sorter capable of measuring 20 parameters including FSC and SSC, and can sort up to 4 distinct populations simultaneously, with different cell collection receptacles including tubes, slides, and 6/96/384 well plates.  Thermal control is installed on the sorter to maintain sample temperature at 4°C, room temperature or 37°C.  The instrument is housed in a class 2 biosafety cabinet and is operated by members of the TTP.

FACS Aria Fusion

 

BD Aria III

Aria III-A is a 3-laser sorters capable of measuring 16 parameters including FSC and SSC. 

Aria III-B is a 4-laser sorters capable of measuring 18 parameters including FSC and SSC.

Both sorters are able to sort up to 4 different populations simultaneously, with different cell collection receptacles including tubes, slides, and 6/96/384 well plates. Thermal control is installed on the sorters to maintain sample temperature at 4°C, room temperature or 37°C. The sorters are operated by members of the TTP.

FACS Aria III

 

Services

Cell Sorting Service

The facility runs a cell sorting service with facility members of staff working closely with researchers to help develop effective sorting experiments.

Mass Cytometry Service

The facility runs a mass cytometry service running samples for researchers in the institute and ensuring optimal performance of the instrument.

Panel Design and Project Development

The facility provides expertise in panel design and project development helping researchers to get the most out of the instrumentation and the fluorescence/mass technology currently available.

Instrument Training

Training is provided on the analysers to researchers of all levels with the aim of making the state of the art technology accessible to researchers with varying level of expertise.

Software available
  • FlowJo
  • FACSDiva
  • Cytobank

Resources

Guides

 

Panel Design tools

www.chromocyte.com

Spectral Viewers