UCL Division of Biosciences


Course Content

Carrying out an experiment in the Gout Lab

Lab Skills

  • Good cell culture practice, including safety procedures, control of facilities, equipment, reagents
  • Qualitative characteristics of mammalian cell cultures:  cell counting and analysis
  • Cryopreservation and cell banking
  • Sterility and microbial contamination tests
  • Maintenance and manipulation of  primary and established cell lines under various experimental conditions (mitogenic stimulation, exposure to cellular stresses and major signal transduction inhibitors)
  • Transient transfection of mammalian cells, expressing wild type and mutant proteins
  • Immunofluorescent analysis of subcellular localisation of transiently expressed proteins
  • Quantitative analysis of immunofluorescent images by computer software
  • Assess the viability and proliferation of cells grown under various experimental conditions using the MTT Cell Proliferation Assay
  • Thermofisher will demonstrate the EVOS imaging system

Course Lectures

  • Whole tissue/cell techniques (immunofluorescent and immunoelectron microscopy, subcellular fractionation, FACS analysis etc)
  • The control of cellular metabolism and growth by signalling pathways
  • Interaction between cells and their environments: general principles in cell signalling and signal transduction
  • Regulation of cell survival and proliferation in mammalian cells
  • Understanding the biology of ageing and regulation of cellular metabolism
  • Stem cell biology in health and disease
  • Human pluripotent stem cells in culture

RSB logo

approved by RSB for 72 credit points