Course Content

Good cell culture practice, including safety procedures, control of facilities, equipment, reagents
Qualitative characteristics of mammalian cell cultures: cell counting and analysis
Cryopreservation and cell banking
Sterility and microbial contamination tests
Maintenance and manipulation of primary and established cell lines under various experimental conditions (mitogenic stimulation, exposure to cellular stresses and major signal transduction inhibitors)
Transient transfection of mammalian cells, expressing wild type and mutant proteins
Immunofluorescent analysis of subcellular localisation of transiently expressed proteins
Quantitative analysis of immunofluorescent images by computer software
Assess the viability and proliferation of cells grown under various experimental conditions using the MTT Cell Proliferation Assay
Thermofisher will demonstrate the EVOS imaging system

Whole tissue/cell techniques (immunofluorescent and immunoelectron microscopy, subcellular fractionation, FACS analysis etc)
The control of cellular metabolism and growth by signalling pathways
Interaction between cells and their environments: general principles in cell signalling and signal transduction
Regulation of cell survival and proliferation in mammalian cells
Understanding the biology of ageing and regulation of cellular metabolism
Stem cell biology in health and disease
Human pluripotent stem cells in culture

approved by RSB for 72 credit points