Macromolecules can change shape when immobilised on a sensor surface and then subsequently treated with different buffers, small ligands or other macromolecules. These are measurable using DPI. This approach can often provide vital clues on bimolecular mechanisms, especially when considering interactions involving proteins immobilised on cell surfaces. Information on dissociation constants is also obtained. Apart from the need to immobilise the partner on a chip surface, the method is artefact-free and does not require the use of any labelling.
The Farfield dual polarisation interferometer instrument (purchased in April 2009) was funded by a CIF bid made jointly between Professor Steve Perkins and Professor John Ward in Structural and Molecular Biology.
It should be noted that we possess analytical ultracentrifuge equipment which makes complementary measurements in solution (AUC) and surface Plasmon resonance which also makes measurements on a sensor chip (SPR).
Room SB16 in the Darwin Subbasement
- Farfield dual polarisation interferometer
- Linux server with 4 Tb disk capacity for data archive
Real-time data following structural events during molecular function and interaction:
- Mechanisms of disease action
- Protein and biomolecule interactions
- With other macromolecules (proteins, DNA, lipids, sugars)
- With small molecules (inhibitors, metal ions)
- Aggregation and inhibition
- Protein folding and conformational changes
- Polymer, surfactant, protein interactions
- Lipids and membrane proteins
Negotiable. We usually charge £60 per day (+ VAT if required) for maintenance to those with grant support. Users should purchase their own sensor chips. A typical project carried out to completion may cost about £500-£1000. Please contact us if you need costs for a grant application.
We anticipate that either we will run the samples for you, or users will have to arrange for proper training. Data processing is the users' responsibility and we can usually provide users with either access multiuser PC's or with their data and the necessary software. The manuals are available as PDF files.