UCL Division of Biosciences


Cystic Fibrosis Bicarbonate Centre

The Cystic Fibrosis Bicarbonate Centre is a new interdisciplinary research initiative linking labs from four prestigious universities. We will focus on how bicarbonate permeation is affected in people with cystic fibrosis, in their airways and beyond.

In health, the CFTR ion channel regulates flow of chloride and bicarbonate ions across epithelial cell layers that line airways and intestines, as well as ducts in the pancreas and in the liver. Bicarbonate has antimicrobial effects in the airways and controls mucus release and fluidity in both airways and gut. Bicarbonate in bile and pancreatic juice is crucial for the breakdown and absorption of fats. In people with cystic fibrosis (CF) CFTR is missing or defective. New CFTR-targeting modulator drugs have been developed, but they primarily optimise CFTR’s chloride conductance. There is now evidence that, at least in bile ducts, current therapies restore chloride but not bicarbonate flow.

This interdisciplinary centre is formed by an international team of researchers with different expertise, coming together to investigate CFTR-dependent bicarbonate secretion.

Five PhD students, supported by five different labs, each one exploiting/developing cutting-edge technologies, will join forces to address these questions:

  • How is bicarbonate flow controlled in different organs? 
  • How do CFTR mutations and new CFTR modulator drugs affect it? 
  • How does its absence contribute to CF symptoms? 
  • Most important, how can we improve modulator therapy to better rescue normal transepithelial HCO3 fluxes, and restore the fizz for people with CF? 


Project oneDevelopment of high-content fluorescence assays to rapidly monitor CFTR-mediated bicarbonate flow - how do CFTR mutations and modulator drugs affect it?
Lead academic: Paola Vergani 

Project two: Investigating the rescue of cystic fibrosis-causing mutations by CFTR modulators with patch-clamp electrophysiology.
Lead academic: David Sheppard 

Project three: Work on stem-cell derived epithelia (2D organoids mimicking gut, pancreas and liver ducts), to investigate transcriptome and transepithelial anion fluxes.
Lead academic: Marcel Bijvelds

Project fourApply nanosensing technology (SICM) to examine the impact of CFTR HCO3- transport in controlling the volume, pH and viscoelasticity of cell secretions and the importance of this for health.
Lead academic: Guy Moss 

Experimental approaches exploiting monolayers derived from organoids. Experiments to measure single-channel gating and permeation, transepithelial Cl- and HCO3- currents, hydration and spinnability of luminal secretions (as well as expression studies and pHstat titration) will be run using common experimental conditions, to facilitate reaching integrated, strong interpretations.

Project fiveFocus on airway epithelia and antimicrobial properties of secretions.
Lead academic: Isabelle Sermet-Gaudelus 


Meet the Scientists


Paolo Vergani
David Sheppard

David Sheppard

Bristol University

M.J.C. Bijvelds

Marcel Bijvelds

Erasmus Medical Centre

guy moss

Guy Moss


Isabelle Sermet Gaudelus

Isabelle Sermet Gaudelus

Institut Necker Enfants Malades

Lunavath Jyosthna Naik

Lunavath Jyosthna Naik

Graduate student from the Indian Institute of Science Education and Research devoted to biochemistry and proteomics.

James Charlick

James Charlick

Bristol University Pharmacology graduate and patch-clamp eletrophysiologist.

Saidi Li

Saidi Li

UCL Pharmacology graduate interested in channels and rheology of epithelial secretions.

Yashoda Jayal

Yashoda Jayal

Biomedical Sciences graduate from UCL and Imperial with a keen interest in therapy inovation for complex diseases.

Erdene Baigal

Erdene Baigal

Eötvös Loránd University (ELTE) graduate with an interest in genetic modification.

Hugo de Jonge, in memoriam

Hugo brought us together, excited by preliminary data from his lab, and first suggested a project which was "so close to [his] heart". It was with deep sorrow that we found out the illness had taken him away prematurely.  We will miss him: his almost infinite knowledge of all that is epithelial, his attention to detail, his scientific acuity and, most of all, his friendship.