Dr Marco Beato

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Personal Profile

Name: Marco Beato Email: m.beato@ucl.ac.uk
Title: Dr Tel: 020 7679 3767
Department: Neuro, Physiology & Pharmacology Fax: 020 76797298
Position: Reader in Neuroscience Address: Pharmacology, Medical Sciences Building, UCL South Cloisters, Gower St, London, WC1E 6BT
Research Domain: Basic Life Sciences, Neuroscience Web Page: Personal Web Page

Profile

Research Description

My current research is focussed on the characterization of premotor interneurons in the ventral horn of the spinal cord. Premotor networks are involved in all motor circuits and are responsible for the coordination of locomotor activity and for the execution and tuning of all motor tasks.

My main aim is to describe the properties of the network of premotor interneurons in relation to their function and to characterize individual synaptic connections.

In the past I have studied the kinetic of glycine receptors using single channel recordings and fast concentration jumps techniques, combined with mathematical modeling and kinetic analysis. Now I am using electrophysiological recordings to record from multiple cells and from synaptically connected interneuron-motoneuron pairs in slices or in the en-bloc spinal cord.

In collaboration with Professors Andrew Todd and David Maxwell (University of Glasgow) we have characterized glycinergic premotor interneurons and measured the strength of Renshaw cells synapses onto motoneurons. We will now use a rabies based trans-synaptic tracer to label networks of flexor and extensor related premotor interneurons and to drive the expression of channelrhodopsin. Our aim is to determine a map of functional connectivity and to relate it to the electrophysiological and neurochemical profiles of premotor interneurons.

Quantal analysis methods and kinetic modeling are used to characterize the properties of synapses.

My work is funded by a Royal Society University Research Fellowship, by the Leverhulme Trust and by a BBSRC project grant (joint with the University of Glasgow).


Research Activities

Glycinergic synapses in the ventral spinal cord

Education Description

I regularly give lectures and tutorials on several courses within the Pharmacology and Neuroscience degree programmes, including modules PHAR1001, PHAR2003 and PHOL2006. I organize and assist in the preparation of 6 practical modules.

UCL Collaborators

External Collaborators

Publications

    2014

    • Bhumbra GS, Bannatyne BA, Watanabe M, Todd AJ, Maxwell DJ, Beato M (2014). The recurrent case for the renshaw cell.. J Neurosci, 34(38), 12919 - 12932. doi:10.1523/JNEUROSCI.0199-14.2014

    2013

    • Bhumbra GS, Beato M (2013). Reliable evaluation of the quantal determinants of synaptic efficacy using Bayesian analysis. Journal of Neurophysiology, 109(2), 603 - 620. doi:10.1152/jn.00528.2012

    2012

    • Bhumbra GS, Moore NJ, Moroni M, Beato M (2012). Co-Release of GABA Does Not Occur at Glycinergic Synapses onto Lumbar Motoneurons in Juvenile Mice.. Front Cell Neurosci, 6, 8 - . doi:10.3389/fncel.2012.00008

    2011

    • Papageorgiou G, Beato M, Ogden D (2011). Synthesis and photolytic evaluation of a nitroindoline-caged glycine with a side chain of high negative charge for use in neuroscience. TETRAHEDRON, 67(29), 5228 - 5234. doi:10.1016/j.tet.2011.05.045
    • Marcaggi P, Mutoh H, Dimitrov D, Beato M, Knoepfel T (2011). One Step Towards Understanding Metabotropic Glutamate Receptor Kinetics. CURRENT NEUROPHARMACOLOGY, 9, 39 - 39.
    • Moroni M, Biro I, Giugliano M, Vijayan R, Biggin PC, Beato M, Sivilotti LG (2011). Chloride ions in the pore of glycine and GABA channels shape the time course and voltage dependence of agonist currents.. J Neurosci, 31(40), 14095 - 14106. doi:10.1523/JNEUROSCI.1985-11.2011

    2010

    • Moroni M, Beato M, Sivilotti LG (2010). Molecular Determinants in Glycine and GABA-A Receptors that Sense Intracellular Chloride Concentration. BIOPHYSICAL JOURNAL, 98(3), 704A - 704A.
    • Moroni M, Beato M, Sivilotti LG (2010). Molecular determinants in Glycine and GABA-A receptors that sense intracellular chloride concentrations.
    • Krashia P, Moroni M, Broadbent S, Hofmann G, Kracun S, Beato M, Groot-Kormelink PJ, Sivilotti LG (2010). Human α3β4 neuronal nicotinic receptors show different stoichiometry if they are expressed in Xenopus oocytes or mammalian HEK293 cells. PLoS One, 5(10), - . doi:10.1371/journal.pone.0013611

    2009

    • Houston CM, Bright DP, Sivilotti LG, Beato M, Smart TG (2009). Intracellular chloride ions regulate the time course of GABA-mediated inhibitory synaptic transmission. J Neurosci, 29(33), 10416 - 10423. doi:10.1523/​JNEUROSCI.1670-09
    • Scimemi A, Beato M (2009). Determining the neurotransmitter concentration profile at active synapses. Molecular Neurobiology, 40(3), 289 - 306. doi:10.1007/s12035-009-8087-7
    • Marcaggi P, Mutoh H, Dimitrov D, Beato M, Knopfel T (2009). Optical measurement of mGluR1 conformational changes reveals fast activation, slow deactivation, and sensitization. Proc.Natl.Acad.Sci.U.S.A, 106(27), 11388 - 11393.

    2008

    • Beato M (2008). The time course of transmitter at glycinergic synapses onto motoneurons. Journal of Neuroscience, 28(29), 7412 - 7425.
    • Pitt SJ, Sivilotti LG, Beato M (2008). High intracellular chloride slows the decay of glycinergic currents. Journal of Neuroscience, 28, 11454 - 11467. doi:10.1523/​JNEUROSCI.3890-08.2008

    2007

    • Beato M, Sivilotti LG (2007). Single-channel properties of glycine receptors of juvenile rat spinal motoneurones in vitro. J PHYSIOL-LONDON, 580(2), 497 - 506. doi:10.1113/jphysiol.2006.125740
    • Beato M, Burzomato V, Sivilotti LG (2007). The kinetics of inhibition of rat recombinant heteromeric α1β glycine receptors by the low affinity antagonist SR-95531.. The Journal of Physiology, 580, 171 - 179. doi:10.1113/jphysiol.2006.126888
    • Sivilotti L, Colquhoun D, Beato M (2007). Single channel properties of glycine receptors from rat spinal motoneurones.
    • Beato M, Sivilotti LG (2007). Single channel properties of glycine receptors of juvenile rat spinal motoneurones in vitro. The Journal of Physiology, 580(2), 497 - 506. doi:10.1113/jphysiol.2006.125740
    • Beato M, Sivilotti LG (2007). Single-channel properties of glycine receptors of juvenile rat spinal motoneurones in vitro.. J Physiol, 580(Pt. 2), 497 - 506. doi:10.1113/jphysiol.2006.125740

    2006

    • Beato M, Sivilotti LG (2006). Single channel properties of mature rat glycine receptors from spinal motoneurones.
    • Broadbent SD, Groot-Kormelink PJ, Krashia PA, Harkness PC, Millar NS, Beato M, Sivilotti L (2006). Incorporation of the ß3 subunit has a dominant negative effect on the function of recombinant central-type neuronal nicotinic receptors.. Molecular Pharmacology, 70, 1350 - 1357. doi:10.1124/mol.106.026682
    • Groot-Kormelink PJ, Broadbent SD, Beato M, Sivilotti LG (2006). Constraining the expression of nicotinic acetylcholine receptors using pentameric constructs. Molecular Pharmacology, 69, 558 - 563. doi:10.1124/mol.105.019356

    2005

    • Miller PS, Beato M, Harvey RJ, Smart TG (2005). Molecular determinants of glycine receptor alpha beta subunit sensitivities to Zn2+ inhibition. Journal of Physiology, 566, 657 - 670.

    2004

    • Burzomato V, Groot-Kormelink P, Colquhoun D, Beato M, Sivilotti L (2004). Activation of heteromeric alpha 1/beta rat glycine receptors.
    • Beato M, Groot-Kormelink PJ, Colquhoun D, Sivilotti LG (2004). The activation mechanism of α1 homomeric glycine receptors. Journal of Neuroscience, 24, 895 - 906. doi:10.1523/JNEUROSCI.4420-03.2004
    • Burzomato V, Beato M, Groot-Kormelink PJ, Colquhoun D, Sivilotti LG (2004). Single-channel behavior of heteromeric α1β glycine receptors: an attempt to detect a conformational change before the channel opens. Journal of Neuroscience, 24, 10924 - 10940.
    • Colquhoun D, Dowsland KA, Beato M, Plested AJ (2004). How to impose microscopic reversibility in complex reaction mechanisms. Biophysical Journal, 86, 3510 - 3518. doi:10.1529/biophysj.103.038679

    2003

    • Boorman JP, Beato M, Groot-Kormelink PJ, Broadbent SD, Sivilotti LG (2003). The effects of ß3 subunit incorporation on the pharmacology and single channel properties of oocyte-expressed human alpha3ß4 neuronal nicotinic receptors. Journal of Biological Chemistry, 278(45), 44033 - 44040.
    • Burzomato V, Groot-Kormelink PJ, Sivilotti LG, Beato M (2003). Stoichiometry of rat recombinant heteromeric glycine receptors: a mutation approach..
    • Beato M, Groot-Kormelink PJ, Colquhoun D (2003). Kinetic properties of rat recombinant α1 glycine receptors..
    • Burzomato V, Groot-Kormelink PJ, Sivilotti LG, Beato M (2003). Stoichiometry of recombinant heteromeric glycine receptors revealed by a pore-lining region point mutation. Receptors Channels, 9(6), 353 - 361.

    2002

    • Beato M, Groot-Kormelink P, Colquhoun D, Sivilotti LG (2002). Openings of the recombinant rat α1 homomeric glycine receptor as a function of the number of agonist molecules bound. Journal of General Physiology, 119, 443 - 466. doi:10.1085/jgp.20028530
    • Groot-Kormelink PJ, Beato M, Finite C, Harvey RJ, Sivilotti LG (2002). Achieving optimal expression for single channel recording: a plasmid ratio approach to the expression of alpha1 glycine receptors in HEK293 cells. Journal of Neuroscience Methods, 113, 207 - 214. doi:10.1016/S0165-0270(01)00500-3

    2001

    • Marchetti C, Beato M, Nistri A (2001). Alternating rhythmic activity induced by dorsal root stimulation in the neonatal rat spinal cord in vitro. The Journal of Physiology, 530(Pt 1), 105 - 112.
    • Beato M, Groot-Kormelink PJ, Colquhoun D, Sivilotti LG (2001). Detection of a low liganded open state of rat recombinant α1 glycine receptors through high resolutions single channel recording.
    • Beato M, Groot-Kormelink PJ, Sivilotti LG (2001). Concentration dependence of single channel currents through rat recombinant α1 glycine receptors..
    • Marchetti C, Beato M, Nistri A (2001). Evidence for increased extracellular K(+) as an important mechanism for dorsal root induced alternating rhythmic activity in the neonatal rat spinal cord in vitro. Neuroscience Letters, 304(1-2), 77 - 80.
    • Beato M, Groot-Kormelink PJ, Colquhoun D, Sivilotti LG (2001). Concentration dependence of single channel currents through rat recombinant alpha 1 glycine receptors.

    1999

    • Beato M, Nistri A (1999). Interaction between disinhibited bursting and fictive locomotor patterns in the rat isolated spinal cord. Journal of Neurophysiology, 82(5), 2029 - 2038.

    1998

    • Beato M, Nistri A (1998). Serotonin-induced inhibition of locomotor rhythm of the rat isolated spinal cord is mediated by the 5-HT1 receptor class. Proceedings of the Royal Society B: Biological Sciences, 265(1410), 2073 - 2080.
    • Bracci E, Beato M, Nistri A (1998). Extracellular K+ induces locomotor-like patterns in the rat spinal cord in vitro: comparison with NMDA or 5-HT induced activity. Journal of Neurophysiology, 79(5), 2643 - 2652.

    1997

    • Bracci E, Beato M, Nistri A (1997). Afferent inputs modulate the activity of a rhythmic burst generator in the rat disinhibited spinal cord in vitro. Journal of Neurophysiology, 77(6), 3157 - 3167.
    • Beato M, Bracci E, Nistri A (1997). Contribution of NMDA and non-NMDA glutamate receptors to locomotor pattern generation in the neonatal rat spinal cord. Proceedings of the Royal Society B: Biological Sciences, 264(1383), 877 - 884.