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4 YEAR PhD IN NEUROSCIENCE
Anne Stephenson
School of Pharmacy
Biochemistry of ion channel receptors
We study the structure, function and regulation of GABAA receptors, N-methyl-D-aspartate (NMDA) receptors and their associated proteins. We employ biochemical, immunological and molecular biological methodology to address receptor subunit complements and stoichiometries, receptor assembly and trafficking.
AVAILABLE PROJECTS
1. To study the molecular interactions between NMDA receptors and the MAGUK family of clustering proteins. The project will involve the biochemical characterization of NMDA receptors and MAGUK proteins co-expressed in mammalian cells. It will utilize immunochemical and molecular biological methodology that includes site-directed mutagenesis.
2. GRIF-1, GABAA receptor interacting factor, is a novel, 913 aa protein discovered and cloned in my laboratory from a yeast two-hybrid screen searching for GABAA receptor targeting molecules. The project is to elucidate the function of GRIF-1 by the identification of GRIF-1 associated proteins using proteomic technologies.
SELECTED PUBLICATIONS
Rutter, A.R., Freeman, F.M. and Stephenson, F.A. (2002)
Further characterization of the molecular interaction between PSD-95 and NMDA receptors: the effect of the NR1 splice variant and evidence for modulation of channel gating.
J. Neurochem. (2002) J. Neurochem. 81, 1298-1307.
Beck, M., Brickley, K., Wilkinson, H., Sharma, S., Smith, M., Chazot, P.L., Pollard, S. and Stephenson, F.A. (2002)
Identification, molecular cloning and characterization of a novel GABAA receptor associated protein, GRIF-1 J.
Biol. Chem. (2002) In the press
Hawkins, L.M., Chazot, P.L. and Stephenson, F.A. (1999)
Biochemical evidence for the co-association of three NR2 subunits in recombinant N-methyl-D-aspartate receptors.
J. Biol. Chem. (1999) 274, 27211-27218.
More: http://www.pharmacy.ac.uk/paspl_sp.html?&no_cache=1&sword_list[]=anne&sword_list[]=stephenson
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