Viral Cell Biology
As obligate intracellular parasites viruses intimately rely on host cell factors for all stages of their replication. Our group is interested in deciphering the complex interactions that occur between poxviruses and their host cells during all stages of the infection cycle. We investigate how viruses initiate their internalization by endocytosis, and how they utilize transport within cellular endocytic systems to their advantage. We are also analyzing how the viral genome and accessory proteins escape into the cytosol, and how replication proceeds.
For these we combine cellular, molecular, and virological techniques with state-of-the-art technologies such as automated small compound and image-based siRNA screening, advanced proteomics, super-resolution, live cell microscopy, and electron microscopy. Our particular interests lie in uncovering novel mechanisms by which viruses subjugate host cell functions to facilitate their entry, replication, and spread.
Novy K, et al (2018). Proteotype profiling unmasks a viral signalling network essential for poxvirus assembly and transcriptional competence. Nat Microbiol. May;3(5):588-599. doi: 10.1038/s41564-018-0142-6.
Culley S, et al (2018). Quantitative mapping and minimization of super-resolution optical imaging artifacts. Nat Methods. Feb 19. doi: 10.1038/nmeth.4605.
Martínez-Martín D, et al (2017). Inertial picobalance reveals fast mass fluctuations in mammalian cells. Nature. Oct 25;550(7677):500-505. doi: 10.1038/nature24288.
Yakimovich A, et al (2017). Inhibition of Poxvirus Gene Expression and Genome Replication by Bisbenzimide Derivatives. J Virol. Aug 24;91(18). pii: e00838-17. doi: 10.1128/JVI.00838-17.
Gray RD, et al (2016). Open-source single-particle analysis for super-resolution microscopy with VirusMapper. J Vis Exp. 2017 Apr 9;(122). doi: 10.3791/55471.
Medical Research Council
European Research Council
Cytoskeleton, Signalling pathways, Membrane trafficking, Viral pathogens
Light microscopy, Electron microscopy, Super-resolution microscopy, High-content screening, Electron microscopy
Artur Yakimovich (Research Associate)
David Albrecht (Research Associate)
Jennifer Wood (Research Associate)
Victoria Gould (Research Associate)
Susanna Bidgood (Research Associate)
Moona Huttunen (Research Associate)
Melanie Krause (PhD student)
Harriet Mok (PhD student)
Noelia Garcia Calavia (PhD student)
Caroline Martin (PhD student)
Jerzy Samolej (PhD student)
Corina Beerli (PhD student)
Robert Gray (PhD student)
Laura Pokorny (PhD student)
Mark Marsh (LMCB, UK)
Ricardo Henriques (LMCB, UK)
Janos Kriston-Vizi (LMCB, UK)