Phosphate is an essential component of life. It participates in the structure of the cell as a part of phospholipids, in the regulation of enzymatic reactions as a post-translational modification, in the inheritance of genetic material as a part of nucleic acids, and in most of biochemical reactions inside the cells as part of ATP, the main energy currency. My research brings together metabolism and signaling and focuses on how eukaryotic cells sense intracellular phosphate, how they deal with phosphate stores, and how they regulate the size of the different phosphate pools (inositol polyphosphate, inorganic polyphosphate, nucleotides pool, free phosphate). I am using two model systems to tackle the problem: yeast (Saccharomyces cerevisiae, Schizosaccharomyces pombe) and the social amoeba Dictyostelium discoideum.