Dr Mark Lowdell

What is a kidney transplant?
What is a kidney transplant
What is a liver transplant?
What is a liver transplant
What is leukaemia?
What is leukaemia
Research projects
Mark Lowdell AMark Lowdell B

Dr Mark Lowdell

Director of Cellular Therapeutics and RFH/UCL Cancer Biobank

Dr. Mark Lowdell

Contact

Dr Mark W Lowdell
Institute of Immunity and Transplantation
Centre for Cell, Gene & Tissue Therapeutics
Royal Free Hospital
Pond Street
London NW3 2QG
UK
m.lowdell [at] ucl.ac.uk
T. +44 (0)20 7830 2183

Research area

Translational cellular immunotherapy and regenerative medicine

Research summary

My group is focussed on the development of cell and tissue medicines for immunotherapy and transplantation. Our work ranges from basic science of how human natural killer cells target tumours to make our anti-cancer immunotherapy better through the effect of stress and strain on human stem cell attachment and differentiation on 3-D tissue scaffolds. Each of our basic research projects is tightly linked to associated translational projects including clinical trials of adoptive NK-immunotherapy in blood cancers, tissue-engineered tracheal replacements, tissue-engineered laryngeal replacements, oesophageal engineering for transplantation, muscle sheet creation for congenital diaphragmatic hernia and engineered epithelial sheets.
In partnership with colleagues we are developing a iPS cell therapy for macula dystrophy and a genetically modified MSC immunotherapy to target lung cancer.
The CCGTT is the foremost academic facility for the manufacture of cell and tissue medicines, so-called Advanced Therapy Medicinal Products (ATMPs), in the UK. We are licensed by the UK MHRA and HTA for the procurement, production and storage of human cells & tissues for therapy and ATMP manufacture.
The CCGTT incorporates the UCL/RFH BioBank within the IIT which stores both therapeutic and non-therapeutic research samples for clinical trials and collaborative projects as a resource across UCL Partners.

Patient involvement

As a non-clinical lecturer and consultant scientist I have no direct patient contact. I hold Qualified Person status for the legal release of Investigational cell and gene therapies, one of only two academics in the UK. This greatly facilitates our ability to support early phase clinical trials in ATMPs. I sit on the clinical development team and clinical trial team of all of the ATMP trials in which we are involved and I am a consultant to many commercial cell and gene therapy trials in immunotherapy and regenerative medicine.

Group members

Dr Edward Samuel PhD              
Dr May Sabry PhD               
Dr Owen Bain DocEng, MPharm           
Maryam Sekhavat               
Fiona O'Brien                   
Carla Carvalho                   
Arjman Aziz                   
Amrita Jugurnauth               
Kathryn Knapp

Janet North
Kwok Pang
Sam Jide-Banwo
Joseph Wood
Fahim Ghourbandi
Toby Proctor
Holly Bartlett
Melinda Ductor Peters

Recent publications

Beloki L, Ramirez N, Olavarria E, Samuel ER, Lowdell MW. (2014) Manufacturing of highly functional and specific T cells for adoptive immunotherapy against virus from G-CSF-mobilised donors. Cytotherapy 2014:e-pub 18 June

Samuel ER, Beloki L, Newton K, Mackinnon S, Lowdell MW (2014) Isolation of highly suppressive CD25+FoxP3+ T regulatory cells from G-CSF-mobilized donors with retention of cytotoxic anti-viral CTLs: application for multi-functional immunotherapy post stem cell transplantation. PLoS One. 2014 Jan 17;9(1):e85911

Träger U, Andre R, Lahiri N, Magnusson-Lind A, Weiss A, Grueninger S, McKinnon C, Sirinathsinghji E, Kahlon S, Pfister EL, Moser R, Hummerich H, Antoniou M, Bates GP, Luthi-Carter R, Lowdell MW, Björkqvist M, Ostroff GR, Aronin N, Tabrizi SJ. (2013) HTT-lowering reverses Huntington's disease immune dysfunction caused by NFκB pathway dysregulation. Brain. 2014 Mar;137(Pt 3):819-33. doi

Sabry M, Lowdell MW (2014) Tumour-primed NK cells; waiting for the green light. Frontiers in Immunology; 2013: 4:1-7.

Träger U, Magnusson A, Lahiri Swales N, Wild E, North J, Lowdell M, Björkqvist M. (2013) JAK/STAT Signalling in Huntington's Disease Immune Cells. PLoS Curr. 2013 Dec 13;5.

Warne E, Birchall M, Lowdell M (2013) Tissue banking in ENT: Challenges and Methods.
J Laryngology & Otology; e-pub. doi:10.1017/S0022215113001321

Pearce KF, Hildebrandt M, Greinix H, Scheding S, Koehl U, Worel N,  Apperley J, Edinger M,  Hauser A,  Mischak-Weissinger E, Dickinson AM, Lowdell MW (2013) The Regulation of Advanced Therapy Medicinal Products in Europe and the Role of Academia. Cytotherapy; 2013 Oct 8 – e-pub

Knight  A, Arnouk H, Britt W,  Gillespie GY, Cloud GA, Harkins L, Su Y, Lowdell MW, Lamb LS. (2013) CMV-Independent Lysis of Glioblastoma by ex vivo expanded/activated Vδ1+ γδ T cells. PLoS One; 2013 – e-pub

Hachani R, Lowdell MW, Birchall M, Thanh NKT (2013)
Tracking stem cells in tissue-engineered organs using magnetic nanoparticles. Nanoscale. 2013 Dec 7;5(23):11362-73

Fishman JM, Lowdell MW, Ansari T, North J, Sibbons P, Birchall MA, De Coppi P.(2013) Immunomodulatory effect of a decellularized skeletal muscle scaffold in a discordant xenotransplantation model. PNAS; 110:14360-14365

Partington L, Mordan NJ, Mason C, Knowles JC, Kim HW, Lowdell MW, Birchall MA, Wall IB. (2013) Biochemical changes caused by decellularization may compromise mechanical integrity of tracheal scaffolds. Acta Biomater. 2013 Feb;9(2):5251-61.

Birchall MA, Elliott MJ, Lowdell M, De Coppi P. (2013) Stem-cell-based, tissue-engineered tracheal replacement in a child - Authors' reply. Lancet. 20;381(9861):113

Samuel E, Newton K, Mackinnon S, Lowdell MW. (2013) Successful isolation and expansion of CMV reactive T cells from G-CSF mobilised donors that retain a strong cytotoxic effector function. Br Journal of Haematology; 160:87-100.

Knight A, Mackinnon S, Lowdell MW. (2012) Human Vdelta 1 gamma-delta T cells exert potent specific cytotoxicity against primary multiple myeloma cells. Cytotherapy; 14:1110-1118.

Lowdell MW (2012) Book review “Natural Killer T Cells - Balancing the regulation of tumor immunity”, British Journal of Cancer; 107:1795-6.

Lowdell MW, Birchall M, Thrasher AJ.(2012) Use of one-off, compassionate use, advanced therapy medicinal products (ATMP) as a safe and valid alternative to animal models for pre-clinical data for clinical trial submissions? Lancet; 379:2341.

Elliot M, DeCoppi P, Speggiorin S, Roebuck D, Butler C, Samuel E, Crowley C, Maclaren C, Cochrane L, Jephson C, Janes S, Beaumont NJ, Cogan T, Hsuan J, Lowdell MW, Birchall M. (2012) Stem-cell based tissue engineered tracheal replacement in a child: case report and twenty month follow-up. Lancet; 380:994-1000.

Oseni AO, Crowley C, Lowdell M, Birchall M, Butler PE, Seifalian AM.  (2012) Advancing nasal reconstructive surgery: the application of tissue engineering technology. J Tissue Engineering and Regenerative Medicine; e-pub

Sabry M, Tsirogianni M, Bakhsh IA, North J, Sivakumaran J, Giannopoulos K, Anderson R, Mackinnon S and Lowdell MW.(2011) Leukemic priming of resting NK cells is KIR independent but requires CD15-mediated CD2 ligation and natural cytotoxicity receptors. J.Immunology; 187:6227-6234.

Katodritou E, North J, Kottaridis P, Verrou E, Gastari V,  Chadjiaggelidou C, Sivakumaran J, Jide-Banwo S, Tsirogianni M, Zervas K, Lowdell MW. (2011) Tumor-primed Natural Killer Cells from Patients with Multiple Myeloma Lyse Autologous, NK-resistant, Bone Marrow-derived Malignant Plasma Cells. Am J Hematology; 86:967-973

Peggs K, Thomson K, Samuel E, Dyer G, Armoogum J, Pang K, Mackinnon S, Lowdell MW. (2011) Directly selected cytomegalovirus-reactive donor T cells confer rapid and safe systemic reconstitution of virus-specific immunity following stem cell transplantation. Clinical Infectious Diseases; 52:49-57. PMID: 21148519

Lowdell MW & Samuel E
Processing Cells for Transplantation
Chapter 16 in “Tissue & Cell Processing – an essential guide” 1st edition
Ed: Deidre Fehily, Scott Brubaker, John Kearney, Lloyd Wolfinbarger
Publ: Wiley - Blackwell, Oxford, UK, 2011.

Older publications

Knight A, Travers PJ, Grace S, Kottaridis P, Mackinnon S, Madrigal AJ, Lowdell MW. (2009)
The role of Vdelta2-negative  T cells during cytomegalovirus reactivation in recipients following allogeneic stem cells transplantation. Blood; 116(12):2164-72 PMID: 20576814

Steele AJ, Prentice AG, Cwynarski K, Hoffbrand AV, Hart SM, Lowdell MW, Samuel ER, Wickremasinghe RG. (2010) The JAK3-selective inhibitor PF-956980 reverses the resistance to cytotoxic agents induced by interleukin-4 treatment of chronic lymphocytic leukemia (CLL) cells. Blood; e-pub PMID: 19515722

Orti G, Lowdell M, Fielding A, Samuel E, Pang K, Kottaridis P, Morris E, Thomson K, Peggs K, Mackinnon S, Chakraverty R. (2009) Phase I study of high-stringency CD8 depletion of donor leukocyte infusions after allogeneic hematopoietic stem cell transplantation. Transplantation; 88:1312-1318. PMID: 19996931

Lowdell MW (2009). Advanced Therapy Medicinal Products; what are they and why are they important to you? BSBMT News; 6:1-2.

Steele AJ, Prentice AG, Hoffbrand AV, Yogashangary BC, Hart SM, Lowdell MW, Samuel ER, North JM, Nacheva EP, Chanalaris A, Kottaridis P, Cwynarski K, Wickremasinghe RG. (2009) 2-phenylacetylenesulfonamide (PAS) induces p53-independent apoptotic killing of B-chronic lymphocytic leukemia (CLL) cells. Blood;114:1217-25. PMID: 19515722

Kogelberg H, Tolner B, Thomas GJ, Di Cara D, Minogue S, Ramesh B, Sodha S, Marsh D, Lowdell MW, Meyer T, Begent RH, Hart I, Marshall JF, Chester K. (2008) Engineering a single chain Fv antibody to alpha V beta 6 integrin using the specificity-determining loop of foot-and-mouth disease virus. J.Mol.Biol; 382:385-401. PMID: 18656482

Björkqvist M,  Wild EJ, Thiele J, Silvestroni A, Andre R, Lahiri N, Raibon E, Lee RV, Benn CL, Soulet D, Magnusson A, Woodman B, Landles C, Pouladi MA, Hayden MR, Khalili-Shirazi A, Lowdell MW, Brundin P, Bates GP, Leavitt BR, Möller T and Tabrizi SJ (2008). A novel pathogenic pathway of immune activation detectable before clinical onset in Huntington’s disease. J Exp Med;205:1869-77. PMID: 18625748

Lowdell MW & Morris E
Recent Advances in Clinical Cellular Immunotherapy
Chapter 41 in “Practical Transfusion Medicine – 3rd edition”
Ed: Mike Murphey & Derwood Pamphilon
Publ: Wiley - Blackwell, Oxford, UK, 2008.

Mattes FM, Vargas A, Hainsworth EG, Sweny P, Nebbia G, Lowdell M, Klenerman P, Phillips RE, Griffiths PD, Emery VC. (2008) Functional impairment of cytomegalovirus specific CD8 T-cells predicts high level replication after renal transplantation. Am J Transplantation;8:990-9. PMID: 18325078

Mackinnon S, Thomson K, Verfuerth S, Peggs K, Lowdell MW. (2008) Adoptive Cellular Therapy for Cytomegalovirus Infection following Allogeneic Stem Cell Transplantation using Virus-Specific T-cells.
Blood Cells Mol Dis.;40:63-7. PMID: 17869548

Lindsey WB, Lowdell MW, Marti GE, Abbasi F, Zenger V, King KM, Lamb LS. (2007) CD69 expression as an index of T cell function: assay standadization, validation and use in monitoring immune recovery. Cytotherapy;9:123-32. PMID: 17453964  

Kogelberg H, Tolner B, Sharma SK, Lowdell M, et al. (2007) Clearance Mechanism of a Mannosylated Antibody-Enzyme Fusion Protein used in Experimental Cancer Therapy. Glycobiology; 17:36-45. PMID: 17000699

North J, Bakhsh I, Marden C, Pittman H, Addison E, Navarrete C, Anderson R, Lowdell MW. (2007) Tumor-primed human natural killer cells lyse NK-resistant tumor targets: evidence for a two-stage process in resting NK cell activation. Journal of Immunology; 178:85-94. PMID: 17182543

Raeiszadeh M, Kopycinski J, Paston SJ, Diss T, Lowdell M, Hardy GAD, Hislop AD, Workman S, Dodi A, Emery V, Webster AD. (2007) The T cell response to persistent herpes virus infections in common variable immunodeficiency.  Clin Exp Immunol; 146:234-242. PMID: 17034575

Laurson J, Selden C, Clements M, Mavri-Damelin D, Coward S, Lowdell M, Hodgson HJF (2007) Putative human liver progenitor cell in explanted liver. Cells Tissues Organs; 186:180-191.

Batten P, Sarathchandra P, Antonio JW, Tay SS, Lowdell MW, Taylor PM, Yacoub MH. (2006) Human Mesenchymal Stem Cells Induce T Cell Anergy and Downregulate T Cell Allo-Responses via the TH2 Pathway: Relevance to Tissue Engineering Human Heart Valves. Tissue Engineering; 12:2263-73. PMID: 16968166

Steele AJ, Jones DT, Ganeshaguru K, Duke VM, Yogashangary BC, North JM, Lowdell MW, Kottaridis PD, Mehta AB, Prentice AG, Hoffbrand AV, Wickremasinghe RG. (2006) The sesquiterpene lactone parthenolide induces selective apoptosis of B-chronic lymphocytic leukemia cells in vitro. Leukemia; 20:1073-1079. PMID: 16628188

Addison EG, North J, Bakhsh I, Marden C, Haq S, Al-Sarraj S, Malayeri R, Wickremasinghe RG, Davies JK, Lowdell MW. (2005) Ligation of CD8α+ve on human Natural Killer cells prevents activation-induced apoptosis and enhances cytolytic activity.  Immunology; 116:354-61. PMID: 16236125

Marks AJ, Cooper MS, Anderson RJ, Orchard KH, Hale G, North JM, Lowdell MW, Ganeshaguru K, Steele AJ, Mehta AB, Wickremasinghe RG. (2005) Selective apoptotic killing of leukemic cell lines by antibody-targeted delivery of an amphipathic peptide. Cancer Research; 65:2373-7 PMID: 15781652

Lowdell MW
Experimental design, data analysis and fluorescence quantitation -
Chapter 5 in “Flow Cytometry – principles and applications”
Ed: Marion G Macey
Publ: Humana Press, New Jersey USA, 2005.

Davies J, Marden C, Sviland L, North J, Lowdell MW (2004) Ex-vivo alloreactive T cell depletion with anti-CD69: effects on anti-viral immunity and donor NK cell function.  Blood Cells, Molecules and Diseases; 33:198-199.

Craig C E H, Quaglia A, Selden C, Lowdell M, Hodgson H, Dhillon A P. (2004) The Histopathology of Regeneration in Massive Hepatic Necrosis.  Seminars in Liver Disease; 24:49-64. PMID: 15085486

Davies JK, Koh MBC, Lowdell MW. (2004) Anti-viral immunity and T-Regulatory cell function are retained following selective alloreactive T cell depletion in both the HLA identical and mismatched settings.  Biology of Blood & Marrow Transplantation;10:259-268.

Jones DT, Addison E, North JM, Lowdell MW, Hoffbrand AV, Mehta AB, Ganeshaguru K, Folarin NI, Wickremasinghe RG. (2004) Geldanamycin and Herbimycin A induce apoptotic killing of B-chronic lymphocytic leukemia cells and augment their sensitivity to cytotoxic drugs.  Blood; 103:1855-61.

Orleans-Lindsay JK, Deru A, Craig JIO, Prentice HG, Lowdell MW. (2003) In vitro co-stimulation with anti-CD28 synergises with IL-12 in the generation of T cell immune responses to leukaemic cells; a strategy for ex-vivo generation of CTL for immunotherapy.  Clin Exp Immunology; 133:467-75.

Lowdell MW (2003) Inside Blood “Out damned spot” – ex-vivo purging revisited.  Blood 102;3860-1.

Lowdell MW (2003) Natural Killer cells in haematopoietic stem cell transplantation.  Transfusion Medicine; 13:399-404.

Smith L & Lowdell MW (2003) Quality issues in stem cell and immunotherapy laboratories.  Transfusion Medicine; 13:417-23.

Davies JK & Lowdell MW (2003) New advances in acute GvHD prophylaxis.  Transfusion Medicine; 13:387-97.

Pamphilon DH & Lowdell MW (2003) Stem cells and immunotherapy. Transfusion Medicine; 13:323-4.

Duarte RF, Chen FE, Lowdell MW, Potter MN, Lamana ML, Prentice HG, Madrigal AJ (2002) Functional impairment of human T-lymphocytes following PHA-induced expansion and retroviral transduction: Implications for gene therapy.  Gene Therapy; 9:1359-68.

Duarte RF, Chen FE, Lowdell MW, Lamana ML, Potter MN, Bueran JA, Prentice HG, Madrigal AJ (2002) Mechanisms of functional impairment of T-lymphocytes following polyclonal for retrovirally mediated gene transfer.  Tissue Antigens; 59:66-66.

Koh MBC, Prentice HG, Corbo M, Morgan M, Cotter FE, Lowdell MW. (2002) Alloantigen specific T cell depletion in a Major Histocompatibility Complex fully-mismatched murine model provides effective graft versus host disease prophylaxis in the presence of lymphoid engraftment.  Br J Haematology; 118:108-116.

Lowdell MW, Craston R, Samuel D, Wood ME, O’Neil E, Saha V, Prentice HG.  (2002) Evidence that continued remission in patients treated for acute leukaemia is dependent upon autologous natural killer cells.  Br J Haematology; 117:821-827.

Tiwari A, Hamilton G, Seifalian AM, Lowdell MW, O’Neill E. (2002) Regarding “Isolation of endothelial cells and their progenitor cells from human peripheral blood” . Journal of Vascular Surgery; 35:827.

Orleans-Lindsay J, Barber LD, Prentice HG, Lowdell MW. (2001) Acute myeloid leukaemia cells secrete soluble factors that inhibit T and NK cell proliferation but not cytolytic  function - implications for the adoptive immunotherapy of leukaemia. Clin Exp Immunology;126:403-411.

Lowdell MW.
Data analysis in flow cytometry –
Chapter 5 in “Cytometric analysis of cell phenotype and function”
Ed: D McCarthy & Marion G Macey
Publ: Cambridge University Press, Cambridge UK, 2001.

Jones DT, Ganeshaguru K, Virchis AE, Lowdell MW, Mehta AB, Prentice HG, Hoffbrand AV, Wickremasinghe RG. (2001) Caspase 8 activation independent of FAS (CD95/APO-1)-signalling may mediate killing of B-chronic lymphocytic leukaemia cells by cytotoxic drugs or  radiation.  Blood; 98:2800-7.

Borras FE, Matthews NC, Lowdell MW, Navarrete CV (2001)  Identification of both myeloid CD1c+ and lymphoid CD11c- dendritic cell subsets in cord blood.  Br J Haematology; 113:925-31.

Hennessy B, North J, Deru A, Llewellyn-Smith N, Lowdell MW.  (2001) The use of Leu3b for the accurate determination of CD4 subsets by measurement of intracellular cytokines.  Cytometry; 44:148-52

Plunkett FJ, Soares MV, Annels N, Hislop A, Ivory K, Lowdell M, Salmon M, Rickinson A, Akbar AN (2001)  The flow cytometric analysis of telomere length in antigen-specific CD8(+) T cells during acute Epstein-Barr virus infection.  Blood;97:700-7.

Adeyemo D, Imtiaz F, Toffa S, Lowdell MW, Wickremasinghe RG, Winslet M (2001) Antioxidants enhance the susceptibility of colon carcinoma cells to 5-fluorouracil by augmenting the induction of the bax protein.  Cancer Letters; 164:77-84.

Lowdell MW (2001)   Allogeneic immunotherapy of acute leukaemia.  
CME Bulletin Haematology; 3:63-66.

Lowdell MW, Lamb L, Hoyle C, Velardi A, Prentice HG (2001) Non-MHC restricted cytotoxic cells – their roles in the control and treatment of leukaemias.  Br J Haematol; 114:11-24.

Lowdell MW, Koh MBC (2000)  Immunotherapy of AML: future directions.
Journal of Clinical Pathology;53:49-54.

Adeyemo D, Imtiaz F, Toffa S, Lowdell M, Winslet M (2000) BAX/BCL-2 ratio is elevated by combined 5FU and antioxidant in colon cancer cells in vitro.  Gut; 46: 11 .

Borthwick NJ, Lowdell M, Salmon M, Akbar A.  (2000)  Loss of CD28 expression on CD8+ T cells is induced by IL-2R-chain signalling cytokines and type 1 interferon and increases susceptibility to activation-induced apoptosis.  International Immunology;12:1005-13.

Lowdell MW, Craston R, Prentice HG. (1999) Generation of autologous immunity to acute myeloid leukaemia and maintenance of complete remission following alpha interferon treatment.  
Cytokines, Cellular & Molecular Therapy; 5:119-21.

Koh MBC, Prentice HG, Lowdell MW. (1999) Selective removal of alloreactive cells from haematopoietic stem cell grafts: graft engineering for GvHD prophylaxis. Bone Marrow Transpl; 23:1071-9.

Han P, Dominguez E, Lowdell MW.
Immunobiology of neonatal NK cells –
Chapter 3 in “Cord Blood Characteristics – role in stem cell transplantation”
Ed: S Cohen, E Gluckman, P Rubinstein, JA Madrigal.
Publ: Martin Dunitz Ltd, London, 1999

Fierabracci A, Hammond L, Lowdell M, Chiovato L, Goode AW, Bottazzo GF, Mirakian R. (1999) The effect of Staphylococcal Enterotoxin B on thyrocyte HLA molecule expression.  J Autoimmunity; 12:305-14.

Morgan C, Ruprai AK, Solache A, Lowdell MW, Price CP, Cohen SBA, Parham PJ, Madrigal JA, Newman DJ. (1998)  The influence of exogenous peptide on 2-microglobulin exchange in the HLA-complex: analysis in real time.  Immunogenetics; 48:98-107.

Lowdell MW, Craston R, Ray N, Koh M, Galatowicz G, Prentice HG (1998).  The effect of T cell depletion with Campath-1M on immune reconstitution after chemotherapy and allogeneic bone marrow transplant as treatment for leukaemia.  Bone Marrow Transpl; 21:679-86.

Craston, R., Koh, M., McDernott, A., Ray, N., Prentice, H.G., Lowdell, M.W. (1997)  Temporal dynamics of CD69 expression on lymphoid cells.  J Immunol Methods;209:37-45.

Lowdell MW, Craston R, Prentice HG (1997)  Understanding the graft-versus-leukaemia reaction: progress towards the immunotherapy of leukaemia. Hematology;2:193-201.

Lowdell MW, Theocharous P (1997)  “Less is more” - the role of purging in haematopoietic stem cell transplantation.  Oncologist; 2:268-74

Hammond, L.J., Lowdell, M.W., Cerrano, P., Goode, A.W., Bottazzo, G.F., Mirakian, R. (1997)  Analysis of apoptosis in relation to tissue distruction associated with Hashimoto’s autoimmune thyroiditis.  J. Pathol;182:138-44.

Bainbridge, D.R., Lowdell, M.W., Hannet, I.M., Strauss, K.W., Karpas, A . (1997)  Can repeated plasma donation by asymptomatic HIV-infected individuals delay the onset of AIDS?  Phil Trans Royal Soc Lond.;352:763-70.

Anderson, R., Macdonald, I., Corbett, T., Hacking, G., Lowdell, M.W., Prentice, H.G. (1997).  Construction and biological characterisation of an interleukin 12 fusion protein:Flexi-12 delivered to acute myeloid leukaemic blasts using adeno-associated virus.  Human Gene Therapy;8:1125-1135.

Lowdell, M.W., Ray, N., Craston, R., Corbett, T., Deane, M., Prentice, H.G.  (1997).  The in vitro detection of anti-leukaemia-specific cytotoxicity after autologous bone marrow transplantation for acute leukaemia.  Bone Marrow Transpl;19:891-897.

Anderson, R.J., Galatowicz, G.,Macdonald, I.D., Lowdell, M.W., Corbett, T.J., Prentice, H.G. (1997) Detection of adeno-associated virus type 2 in sorted human bone marrow progenitor cells.  Experimental Hematology; 25:256-262.

Borthwick N, Akbar A , MacCormac LP, Lowdell MW, Craigen JL, Hassan I, Grundy JE, Salmon M, Yong KL. (1997)  Selective migration of highly differentitated primed T cells, defined by low expression of CD45RB, across human umbilical vein endothelial cells: effects of viral infection on transmigration.  Immunology; 90:272-80.

Theocharous, P., Lowdell, M.W., Jones, A.L., Prentice, H.G. (1997)  
Immunocytochemical Detection of Breast Cancer Cells - A Comparison of Three Attachment Factors.  Journal of Hematotherapy;6:21-29.

Dominguez, E., Lowdell, M.W., Perez-Cruz, I., Madrigal, A., Cohen, S.B. (1997)  Natural killer cell function is altered by freezing in DMSO.  Biochemical Soc Trans; 25:175.

Lowdell, M.W., Bainbridge, D.R. (1996) External quality assurance for CD34 cell enumeration - the results of a preliminary national trial.  Bone Marrow Transplantation;17:840-853.

Nouri, A.M., Lowdell, M.W., Entazami, Z., Tezabwala, B.U., Goode, A., Oliver, R.T.  (1996)  Comparison of immunological changes between subcutaneous and intravenous routes of administration of IL-2 in cancer patients.  Urologia Internationalis;56:1-5.

Lowdell, M.W., Shamim, F., Hamon, M., Macdonald, I.D, Prentice, H.G. (1995) VLA-6 (CDw49f) is an important adhesion molecule in NK cell-mediated cytotoxicity following autologous or allogeneic bone marrow transplantation.  Experimental Hematology ;23:1530-1534

Lowdell, M.W. & Cerrano, P. (1995)  Clinical applications of flow cytometry.
Annals of Experimental & Clin  Medicine;4:165-170

Lowdell, M.W. & Bottazzo, G.F. (1993)
Autoimmunity and insulin-dependent diabetes.
Lancet;341:1378-1379.

Karpas, A., Lowdell, M.W., Jacobson, S.K. & Hill, F. (1992) Inhibition of human immunodeficiency virus and growth of infected T cells by the immunosuppressive drugs cyclosporin A and FK 506.  Proc.Nat.Acad.Sci.;89:8351-55.

Bainbridge, D.R. &  Lowdell, M.W. (1992)  Flow cytometric measurement of absolute cell number by internal standard.  Flow Cytometry Standards Forum;4:3-8.

O'Toole, C.M., Lowdell, M.W., Chargelegue, D. & Colvin, B.T. (1992)  Decline in CTL and antibody responses to HIV-1 p17 and p24 antigens in HIV-1-infected haemophiliacs irrespective of disease progression. A 5-year follow-up study.  AIDS Research and Human Retroviruses;8:1361-8.

MacLean, I.L., Lowdell, M.W., Blake, D.R., Lunec, J., Archer, J.R. (1992) Absence of a specific effect of free radicals on HLA-B27. Ann-Rheum-Dis.;51: 963-4.  

Al-Sakaf, L., Pozzilli, P., Lowdell, M.W., Bingley, P.J., Thomas. J.M.,
Bonifacio, E., Gale, E.A.M. & Bottazzo, G.F. (1992)  Early T-cell defects in pre-type 1 diabetes.  Acta Diabetologica;28:189-192.

McClean, L., Macey, M., Lowdell, M., Badakere, S., Whelan, M., Perret, D. & Archer, J. (1992)  Sulphydryl reactivity of the HLA-B27 epitope. Flow cytometry suggests the free cysteine is often inaccessible.  Annals of the Rheumatic Diseases;51:456-460.

De Giorgi, L., Lowdell, M.W. & Matossian-Rogers, A. (1991) Cellular mechanisms of graft-versus-host disease in a mouse model.  Scand.J.Immunol.;33:567-574.

O'Toole, C.M. & Lowdell, M.W. (1990)  Infection of human T cells with mycoplasma, inhibition of CD4 expression and HIV-1 gp120 glycoprotein binding and infectivity.  Lancet;336:1067.

Kelsey, S.M., Lowdell, M.W. & Newland, A.C. (1990)  IgG subclass levels in immune reconstitution after T cell depleted allogeneic bone marrow transplantation.  Clin.Exp.Immunol.;80:409-412.

Lowdell MW, Lawry J.
Quality Control in Clinical Flow Cytometry -
Chapter 3 in "Flow Cytometry - clinical applications",
ed: Marion G. Macey,
publ: Blackwell Scientific Publications Ltd, Oxford, 1989.

O'Toole, C.M. & Lowdell, M.W. (1989) The serological detection of recombinant vaccinia virus encoded HIV-1 gag proteins in the cytoplasm of human B cells in the absence of cell surface expression.  Serodiagnosis and Immunotherapy in Infect. Dis.;3:329-336.

Roy, V., Veys, P., Jackson, F., Ryan, J., Lowdell, M. & Newland, A.C. (1989)
Adult respiratory syndrome following autologous bone marrow transfusion.
Bone Marrow Transplantation;4:711-712.

Lowdell, M.W. & O'Toole, C.M. (1988) Defects in cell mediated immunity in HIV infection: implications for immunotherapy and vaccine development.
Serodiagnosis and Immunotherapy in Infect. Dis.;2:251-261.

Lowdell, M.W. & Bainbridge, D.R. (1987)  An automated technique to measure breakdown products of C3 with C3d specificities by peak-rate nephelometry.  Med.Lab.Sci.;44:331-333.

Wali, F.A., Bradshaw, E.G., Lowdell, M.W., Tugwell, A.C. & Makinde, V. (1987)
Atracurium and complement activation in man.  Med.Sci.Res.;15:555-556.


Lowdell MW (2003) Inside Blood “Out damned spot” – ex-vivo purging revisited.  Blood 102;3860-1.

Lowdell MW (2003) Natural Killer cells in haematopoietic stem cell transplantation.  Transfusion Medicine; 13:399-404.

Smith L & Lowdell MW (2003) Quality issues in stem cell and immunotherapy laboratories.  Transfusion Medicine; 13:417-23.

Davies JK & Lowdell MW (2003) New advances in acute GvHD prophylaxis.  Transfusion Medicine; 13:387-97.

Pamphilon DH & Lowdell MW (2003) Stem cells and immunotherapy. Transfusion Medicine; 13:323-4.

Lowdell MW (2001)   Allogeneic immunotherapy of acute leukaemia.  
CME Bulletin Haematology; 3:63-66.

Lowdell MW, Lamb L, Hoyle C, Velardi A, Prentice HG (2001) Non-MHC restricted cytotoxic cells – their roles in the control and treatment of leukaemias.  Br J Haematol; 114:11-24.

Lowdell MW, Koh MBC (2000)  Immunotherapy of AML: future directions.
Journal of Clinical Pathology;53:49-54.

Lowdell MW, Craston R, Prentice HG (1997)  Understanding the graft-versus-leukaemia reaction: progress towards the immunotherapy of leukaemia. Hematology;2:193-201.

Lowdell MW, Theocharous P (1997)  “Less is more” - the role of purging in haematopoietic stem cell transplantation.  Oncologist; 2:268-74

Lowdell, M.W. & Cerrano, P. (1995)  Clinical applications of flow cytometry.
Annals of Experimental & Clin  Medicine;4:165-170

Lowdell, M.W. & Bottazzo, G.F. (1993)
Autoimmunity and insulin-dependent diabetes.
Lancet;341:1378-1379.

Lowdell, M.W. & O'Toole, C.M. (1988) Defects in cell mediated immunity in HIV infection: implications for immunotherapy and vaccine development.
Serodiagnosis and Immunotherapy in Infect. Dis.;2:251-261.

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