IPLS Invited speaker - Dr Stephan Preibisch (Albert Einstein College of Medicine, New York, MPI-CBG, Dresden)
13 January 2015, 4:00 pm–5:00 pm
MRC/Laboratory for Molecular Biology Seminar Room
Title: Image reconstruction and transcription imaging in C. elegans
Abstract: Modern developmental biology increasingly relies on imaging large samples like Drosophila embryos with high spatial and temporal resolution (in-toto imaging). These requirements did not only foster the development of new microscopy technologies like Selective Pane Illumination Microscopy (SLIM), but also new algorithms and software capable of reconstructing and analysing these large multi-tile acquisitions. We will present solutions for the stitching of tiled confocal acquisitions, and for the registration, fusion, deconvolution, and analysis of multi-view SPIM acquisitions. In the second part of the talk we will present our current project that combines single molecule transcription imaging and electron microscopy with computational technologies to create an in-toto model of transcriptional regulation in C. elegans by exploiting the high level of stereotypicality. We show preliminary results of our study that focusses on the quantification of change in alternative splicing throughout development.