Centre for Neuroendocrinology
Neuroendocrinology is part of the Division of Medicine

Medicine News

Myelin vital for learning new practical skills

Published: Oct 20, 2014 9:38:58 AM

The Challenge of Chronic Pain 11-13 March 2015

Published: Oct 1, 2014 4:15:14 PM

BSc/MSci Applied Medical Sciences Open Day 30th October 2014

Published: Oct 1, 2014 3:40:18 PM

Techniques

Biochemistry - standard SDS-PAGE and western blotting, prokaryotic and eukaryotic expression and purification of proteins and immunoprecipitation.

Cell biology - immunocytochemistry, immunohistochenistry, tissue preparation using the vibratome, microtome and cryostat, olfactory placode and olfactory bulb explants, live cell imaging, standard and confocal microscopy.

Molecular biology - PCR, extraction of RNA, RT-PCR, cloning, Northern and Southern blotting, in vitro transcription, in situ hybridisation and site-directed mutagenesis.

Cell culture - primary cell culture (GnRH neurons and astrocytes), cell lines and explant cultures.

Model organisms - chick (in ovo manipulations both pharmacological and genetic) and mouse.