"Lentiviral vectors as tools for gene manipulation"
Prof Chris Boshoff
Description of Project:
RNA interference (RNAi) is a novel technique to manipulate gene expression. It involves targeting mRNA for degradation using sequence specific RNA molecules, and so can be tailored for a range of purposes such as inhibiting viral infection, studying gene pathways and inhibiting cancer growth. We used RNAi, delivered with lentiviral vectors based on HIV-1, to inhibit three genes expressed in the latent life cycle of Kaposi's Sarcoma Associated Herpesvirus (KSHV/HHV-8). The effects of this were evaluated both in vitro and in vivo, and these data suggested that RNAi has potential as a therapeutic against virally driven cancers.
Godfrey,A ., Laman,H., & Boshoff,C. (2003) RNA interference: a potential tool against Kaposi's sarcoma-associated herpesvirus. Curr.Opin.Infect.Dis., 16, 593-600.
Godfrey,A. , Anderson,J., Papanastasiou,A., Takeuchi,Y., & Boshoff,C. (2005) Inhibiting primary effusion lymphoma by lentiviral vectors encoding short hairpin RNA. Blood., 105, 2510-2518.
Clements,M.O., Godfrey,A., Crossley,J., Wilson,S.J., Takeuchi,A., & Boshoff,C. (2006) Lentiviral Manipulation of Gene Expression in Human Adult and Embryonic Stem Cells. Tissue Eng., .
Morisco,F., Stigliano,R., Godfrey,A., Leandro,G., Patch,D., Davidson,B., Rolles,K., Dhillon,A., Dhillon,A.P., Quaglia,A., & Burroughs,A.K. (2007) Efficacy of Loco-Regional Ablation Therapy of HCC in a Population of Liver Transplanted Patients. Dig.Dis.Sci., .
Malcles,M.H., Wang,H.W., Koumi,A., Tsai,Y.H., Yu,M., Godfrey,A., & Boshoff,C. (2007) Characterisation of the anti-apoptotic function of survivin-DeltaEx3 during TNFalpha-mediated cell death. Br.J Cancer., 96, 1659-1666.