back to   Richardson home page Kessaris home page last updated November 2023

Request transgenic mice from the Bill Richardson/ Nicoletta Kessaris labs

We thank the UK Medical Research Council, The Wellcome Trust, the European Research Council, the Biotechnology and Biological Sciences Research Council, the US National Institutes of Health and The Royal Society for past and present support.

We have generated a number of transgenic strains, mainly BAC or PAC transgenics by pronuclear injection.   If you wish to obtain these please follow the steps below.

1. email Bill Richardson  (w.richardson@ucl.ac.uk) and/or Nicoletta Kessaris (n.kessaris@ucl.ac.uk) outlining your proposed project in a few sentences.

2. Some popular lines [e.g. Pdgfra-CreER(T2), Fgfr3-iCreER(T2), Sox10-iCreER(T2)] are listed on the UCL Business website https://xip.uclb.com -> Biological Materials, from where you can download and complete an MTA, as well as obtain further information such as genotyping protocols.  Please check xip.uclb.com in any case because one or two lines are listed there that are NOT listed in the table below.

3. Sometimes the line you want will be available closer to you from a third party lab - we can provide contact details.

4. For health status of the mice please contact Matthew Grist (m.grist@ucl.ac.uk) in the first instance.

We normally ship 2 or 3 males of breeding age. We regret that we cannot ship embryos or sperm.

Please note that we depend on you to pay costs. Shipment is organized by Gabi Sturges (gabrielle.turner@ucl.ac.uk), manager of our Biological Services Unit.  Please contact our lab manager Matthew Grist (m.grist@ucl.ac.uk) first.

The following mice are available, as well as some others not listed here: 

Strain	Animal code	Contact	Vector	Made by	References	MTA	Genotyping protocol	Comments/ expression pattern
Dbx1-iCre	H142	Kessaris cc Richardson	PAC	Fogarty/Kessaris	1, 4, 5	Dbx1-iCre	Dbx1-iCre or iCre	Cortico-striatal boundary, spinal cord p1, p2, dP6, dP5
Dlx1-lox-Venus-lox	A666	Kessaris	BAC	Rubin/Alfonsi/ Kessaris	16 (suppl info)	Dlx1-Venus	Dlx-Venus	Forebrain
Emx1-iCre	H151	Kessaris cc Richardson	PAC	Fogarty/Kessaris	3, (suppl info),  4, 5	Emx1-iCre	Emx1-iCre or iCre or use UV lamp at P0-P1*	Cortical neuroepithelium
Emx1-CreER^T2	G199	Kessaris cc Richardson	PAC	Fogarty/Kessaris	3, 5	Emx1-CreER^T2	Emx1-CreER^T2	Cortical neuroepithelium
Fgfr3-iCreER^T2	K121	Kessaris cc Richardson	PAC	Mitsumori/Kessaris	7 (suppl info), 13 (suppl info 1, 2)	Fgfr3-iCreER^T2	iCre	Neuroepithelial cells, SVZ cells, protoplasmic and fibrous astrocytes
Gsh2-iCre	H153	Kessaris cc Richardson	PAC	Kessaris	3, (suppl info),  4, 5, 10, 12	Gsh2-iCre	Gsh2-iCre or iCre	LGE/MGE/CGE
Lhx6-iCre	N104	Kessaris	BAC	Kessaris	4, 6, 16 (suppl info), 17	Lhx6-iCre	iCre or use UV lamp at early stages*	MGE
Msx3-iCre	K133	Kessaris cc Richardson	PAC	Fogarty/Kessaris	Unpublished	Msx3-iCre	Msx3-iCre or iCre or use UV lamp at early stages*	Dorsal spinal cord dP1, dP2, dP3, dP4 and neural crest
Nkx2.2-CreER^T2	H101	Kessaris cc Richardson	PAC	Taveira-Marques/Kessaris	Unpublished	Nkx2.2-CreER^T2	Nkx2.2-CreER^T2	Ventral spinal cord p3
Nkx2.1-iCre	G178	Kessaris cc Richardson	PAC	Kessaris	3, (suppl. info),  4, 10, 12, 16 (suppl info), 17	Nkx2.1-iCre	Nkx2.1-iCre or iCre	MGE
Nkx2.1-iCre-ER^T2	K185	Kessaris cc Richardson	PAC	Kessaris	Unpublished	Nkx2.1-iCre-ER^T2	Nkx2.1-iCre or iCre	MGE
Nkx6.2-iCre	L129	Kessaris	BAC	Kessaris	4	Nkx6.2-iCre	iCre	LGE/MGE boundary, spinal cord
Olig2-Cre	E193	Richardson	Knock-in	Rowitch/Kessaris	3, (suppl. info),  14	Olig2-Cre	Olig2-Cre	Spinal cord pMN, oligodendrocyte lineage
Olig2-NLS-eGFP	H152	Richardson	PAC	Callard/Kessaris	Unpublished	Olig2-NLS-eGFP	Olig2-GFP	Spinal cord pMN, oligodendrocyte lineage
Pdgfra-CreER^T2	H126	Richardson	PAC	Jamen/Kessaris	7 (suppl info), 15	Pdgfra-CreER^T2	Pdgfra-CreER^T2	Oligodendrocyte progenitors postnatally (ectopic dorsal expression in embryo)
Sox1-iCreER^T2	K184	Kessaris cc Richardson	BAC	Kessaris	Unpublished	Sox1-iCreER^T2	iCre	Embryonic neuroepithelium and adult neural stem cells
Sox10-Cre	F155	Kessaris cc Richardson	PAC	Iannarelli/Kessaris	2, 8, 9, 11	Sox10-Cre	Sox10-Cre or use UV lamp at early stages*	Oligodendrocyte lineage, neural crest, Schwann cells
Sox10- iCreER^T2	N388	Richardson	PAC	Paes de Faria/Li	18, (suppl info)	Sox10- iCreER^T2	Sox10- iCreER^T2	as above
Sox10-lox-GFP-STOP-lox-DTA	K152	Kessaris cc Richardson	PAC	Iannarelli/Kessaris	3, (suppl. info)	Sox10- GFP-DTA	Sox10-DTA or use UV lamp at early stages	Oligodendrocyte lineage, neural crest, Schwann cells
Zic4-iCre	A611	Kessaris	BAC	Kessaris/Rubin	16 (suppl info)	Zic4-iCre	iCre	Septal telencephalic and diencephalic regions

* A UV lamp can be used to genotype these mice when a Cre reporter allele expressing a fluorescent protein is also present