When you start the program and click on the button to sequence the exon, you will see the base sequence of the exon, as it appears in the mRNA. You have to decode the sequence to identify the amino acids in the peptide.
The table shows the genetic code, as in mRNA. Click here to download a printable version.
When you have identified all the amino acids in the peptide coded for by this exon, you have to calculate the charge on the peptide at around pH 7 - this will allow you to determine whether or not the variants of this peptide in the mutants that you will work with later could be separated by electrophoresis.
The diagram below shows the structures of the amino acids. Click here to download a printable version.
