Close
Contamination by Mycoplasmas is a constant threat to the integrity of experiments involving cultured cells. An understanding of the mode of contamination has led to the development of cell culture techniques that should ensure freedom from contamination. Yet, the incidence of Mycoplasma contamination (between 15-40%) has not yet declined appreciably over the past 20 years. There are virtually no cell parameters that are not unaffected by at least one of the several Mycoplasma species that regularly contaminate cells. Mycoplasma contamination is easily avoidable. Regular monitoring and appropriate techniques will result in a mycoplasma-free cell culture environment.
The policy at WIBR is to quarantine any cells that come into the Institute and regularly test cell cultures using an external company specialising in Mycoplasma detection. The most sensitive test is the Direct Culture method and along with indirect Hoesht Staining are the only Food and Drug Administration (FDA) approved tests. Details of how you can get your cells tested and the conditions that are required can be downloaded from www.mycoplasma-exp.com
FAQs
Why do I need to test for Mycoplasmas?
If you do not test your cell cultures, all experimental results could potentially be invalidated. It is also good laboratory practice and is advised by all experts in the field of cell culture and suppliers of cell lines.
What Cell Systems do they affect?
Mycoplasma contamination could potentially effect all cell systems, some of which are listed on this downloadable file.
Where should I get my cells from ?
The Institute highly recommends either the ATCC or ECACC for provision of cell lines. Any cells that come from other departments, Institutes or Companies should be quarantined and tested as early as is feasibly possible.
How long before I get my test results back ?
The Direct Culture Method test for Mycoplasma contamination is a 4 week test. However in 99% of cases, contamination is positively seen after 4-5 in culture and will be communicated by the external testing contractor.