UCL Division of Surgery and Interventional Science


Novel spatial, molecular and imaging approaches to analyse 3D tissue models Virtual Workshop

Centre for 3D Models of Health and Disease presents the second of our pair of twinned workshops, which will be held virtually on 27 July 14:00-18:00

Advances in bioengineering have led to a wide array of biomimetic 3D tissue models being used to study human health and disease. To best utilize and understand these exciting new models, it is key that we employ effective endpoint analysis that goes beyond traditional imaging or molecular approaches. In recent years, a range of new methods have been developed to interrogate the complex molecular microenvironment and architecture of 3D tissue models, many of which fall under the umbrella of ‘spatial biology’ – recognized recently as Nature’s method of the year 2020! 

The aim of this virtual workshop is to meet with academic and industry leaders, to discuss new advances in endpoint analysis of 3D tissue models, including spatial transcriptomics, mass-spectrometry imaging, and highly multiplexed immunofluorescence. New approaches such as these allow us to better understand the biology underpinning our 3D tissue models, and we will compare and contrast their utility during round table discussions. We will share expertise, build networks and consider the future directions of this emerging field. 


Novel spatial, molecular & imaging approaches to analyse 3D tissue models virtual workshop 27 July 2021 14:00-18:00


Workshop Agenda: 

14:00-14:10 Introduction and welcome
Dr Susan Heavey, UCL

Academic session:

14:10-14:35 Mass Spectrometry Imaging in Combination with 3D Tissue Models for Early Stage Efficacy and Safety Testing of Drugs and Toxicants

Prof. Malcolm Clench, Sheffield Hallam University

14:35 – 15:00 Imaging organoid responses to drugs

Prof. Trevor Dale, Cardiff University

15:00 – 15:15 Novel methods for investigating stiffness in 3D in vitro models

Ms Auxtine Micalet, UCL

15:15 – 15:40 A technological roadmap for 3D multi-omics and imaging for human disease modelling

Prof Je Hyuk Lee, Cold Spring Harbour Laboratory

15:40 – 16:10 Brainstorming session – what are our unmet needs for endpoint analyses, as 3D tissue model researchers?

Industry session:

16:10 – 16:30 SpheroMatrices: a spheroid tissue microarray (microTMA) platform for multiplexed analysis of 3D microtissue experiments

Dr Simon Plummer, Micromatricies

16:30 – 16:50 Morphology Driven High-Plex Spatial Analysis of Tissue Microenvironments 

Dr. Anthony Zucca, NanoString Technologies inc.

16:50 – 17:05 Unlocking Spatial Biology with Orion

Dr Tad George, RareCyte, Inc

17:05 – 17:20 Multiplex tissue imaging approaches for deep phenotyping with single-cell resolution

Dr Roslyn Lloyd, Akoya Bioscience

17:20 – 17:40 Spatially map and quantify the whole transcriptome in the tissue context with Visium spatial solutions

Dr Cristophe Fleury, 10X Genomics

17:40 – 18:00 Round table discussion – where do we see the future of spatial biology going?


Further presentations to be announced from both academic and industry experts. Brainstorming sessions will be included, for all attendees to share thoughts on the key requirements and future directions of new technologies in the analysis of 3D models.

Please register above to receive joining details for the event.

Please note that this is the second of a pair of twinned events from our centre this summer. You can find out about the first event, titled ‘Engineering advanced 3D (in vitro) models for solid cancer studies,’ here.