FACILITY

Contact the LMCB Light Microscopy Facility for details of how to access UCL's advanced microscopy facilities or search the UCL Research Equipment Catalogue

Zeiss Elyra PS.1

Zeiss Elyra PS.1

 The Elyra is capable of both Structured Illumination Microscopy (SIM) and Photo-Activation Localisation Microscopy (PALM). In addition it can be configured for dSTORM, standard TIRF and wide-field microscopy applications. The microscope stage is enclosed in a blacked out chamber for laser safety reasons and to maintain stability by protecting the specimen from draughts. Super-resolution imaging is recommended and optimal at ambient temperature but stage-top incubation equipment is available for live imaging applications.

Leica gSTED

Leica gSTED


The gSTED microscope is capable of continuous wave Stimulated Emission Depletion (cwSTED) and gated STED. It has a 405 nm diode laser and super-continuum White Light Laser (WLL) for excitation and a 592 nm diode for depletion. The WLL must be used as an excitation source for gated STED because a pulsed laser is required for this technique. Most STED dyes will excite optimally using the WLL, so the 405 should only be needed for confocal imaging. Resolution when using STED is optimal at 23 degrees C. Matching refractive indices is critical and the specially designed STED objective lens is optimally corrected for use with immersion oils at 23 degrees. However, should incubation be required the microscope is housed within a blacked out cage-type incubator. This is used to protect the specimen from room light and draughts. Environmental control is not currently available but will be coming soon.

Page last modified on 22 mar 19 17:03