Flow Cytometry
Flow Cytometry facilities at UCL ensure researchers have timely access to equipment, resources and expertise necessary to undertake world-leading research.
About the STP
The Flow Cytometry Science Technology Platform (STP) at University College London (UCL) is dedicated to providing researchers with access to cutting-edge flow cytometry and cell sorting instrumentation and expertise. It offers a range of services including cell sorting, multi-parameter analysis, and data analysis support.
Researchers can utilize the platform for various applications such as multicolour immunophenotyping, fluorescent protein detection eg GFP, Apoptosis, cell cycle analysis, cell viability, FRET and cytokine detection. The STP is equipped with state-of-the-art flow cytometry analysers/cell sorters with both conventional and spectral based technology and offers training programs to researchers to help them optimize their experiments and analyse their data effectively.
Facilities
Location
Rayne Building, Bloomsbury,
5 University St, London WC1E 6JF
Equipment
The flow cytometry facility consists of 4 analysers 1 highspeed conventional cell sorter and 1 highspeed spectral cell sorter with imaging capabilities.
- BDFACS Discover S8 with BD CellView Cell Sorter
- BDFACS Aria (SORP) Cell Sorter
- Sony ID7000 Spectral Analyser (x2)
- BD LSRFortessa x20 (SORP)
- BD LSRFortessa
- BDFACS Verse
Services
- Cell sorting
- Image based cell sorting
- Spectral based cell sorting
- Single cell sorting for cloning and genomics
- Flow cytometry training
- Spectral flow cytometry analysers
- Conventional flow cytometry analysers
- Panel design/optimisation help and advice
- Experimental design/troubleshooting
- Application/data analysis support
Location
Room X-1096, Lower Ground
Zayed Centre for Research into Rare Disease in Children (ZCR)
20 Guilford Street, London WC1N 1DZ
Equiment
- Sony ID7000 Spectral Analyser
- IR Symphony A5
- Fisher Symphony A5
- LSRII
- CyAn
- CytoFLEX S
- FACSAria III Cell Sorter
- FACSMelody Cell Sorter
- FACSymphony S6 Cell Sorter
- Analysis Computers: FlowJo, FACSDiva, Kaluza, Summit, CytExpert, Sony ID software
Services
- Cell sorting
- Cloning: single cell sorting
- Data acquisition, analysis and interpretation
- User training
- Advice on sample preparation
- User assistance and machine troubleshooting
- Help with design of experiments
- Sourcing of reagents and techniques; finding alternative approaches
- Help with data preparation for presentation and publication
Location
Room 303, 3rd Floor
Paul O'Gorman Building, UCL
72 Huntley Street, London, WC1E 6DD
Equipment
- Cell sorting (BD Aria and Fusion, and Miltenyi MACSQuant® Tyto)
- High throughput flow cytometry analysers (including small particle detection)
- Conventional flow cytometry analysers
- Laminar 96 well plate washer
Services
- Flow cytometry training, seminars and workshops
- Panel design/optimisation help and advice
- Experimental design/troubleshooting
- Basic and High Dimensional Data analysis support
- FlowJo and Cytobank software
Flow Cytometry Core Facility, Institute of Immunity and Transplantation
Location
The Pears Building
Labs 219, 220 and 221, Ground Floor,
Pond Street, London, NW3 2PP
Equipment
The Flow Cytometry Facility consists of 7 analysers and 4 cell sorters, One of which is within our Containment level 3 laboratory.
- BD LSRFortessa (SORP)
- BD LSRII
- Cytek Aurora Spectral Analysers
- BD AriaFusion (SORP) cell sorter
- BDFACS Aria IIu Cell sorter
- Cytek Aurora Spectral Cell sorter
Services
- Cell sort training and support
- Single cell sort training
- Spectral based cell sort training and support
- Flow Cytometry, experimental design, Sample preparation and panel design training, seminars and workshops
- Spectral Flow Cytometry analyser training
- Conventional Flow Cytometry analyser training
- User assistance and instrument troubleshooting
- Experimental design and troubleshooting advice
- Panel design and troubleshooting advice
- Application, data analysis and presentation support and advice