Flow Cytometry
Cell sorting and analysis facilities at UCL ensure researchers have timely access to equipment, resources and expertise necessary to undertake world-leading research.
About the STP
The Cell Sorting and Analysis Science Technology Platform (STP) at University College London (UCL) is dedicated to providing researchers with access to cutting-edge flow cytometry and cell sorting instrumentation and expertise. It offers a range of services including cell sorting, multi-parameter analysis, and data analysis support.
Researchers can utilize the platform for various applications such as multicolour immunophenotyping, fluorescent protein detection eg GFP, Apoptosis, cell cycle analysis, cell viability, FRET and cytokine detection. The STP is equipped with state-of-the-art flow cytometry analysers/cell sorters with both conventional and spectral based technology and offers training programs to researchers to help them optimize their experiments and analyse their data effectively.
Facilities
Location
Rayne Building, Bloomsbury,
5 University St, London WC1E 6JF
Equipment
The flow cytometry facility consists of 4 analysers 1 highspeed conventional cell sorter and 1 highspeed spectral cell sorter with imaging capabilities.
- BDFACS Discover
- BDFACS Aria (SORP)
- Sony ID7000 Spectral Analyser
- BD LSRFortessa x20 (SORP)
- BD LSRFortessa
- BDFACS Verse
Services
- Cell sorting
- Image based cell sorting
- Spectral based cell sorting
- Flow cytometry training
- Spectral flow cytometry analysers
- Conventional flow cytometry analysers
- Panel design/optimisation help and advice
- Experimental design/troubleshooting
- Application support
- Data analysis support
Location
Room X-1096, Lower Ground
Zayed Centre for Research into Rare Disease in Children (ZCR)
20 Guilford Street, London WC1N 1DZ
Equiment
- IR Symphony A5
- Fisher Symphony A5
- LSRII and CyAn
- CytoFLEX S
- FACSAria III Cell Sorter
- FACSMelody Cell Sorter
- Analysis Computers: FlowJo, FACSDiva, Kaluza, Summit
Services
- Cell sorting
- Cloning: single cell sorting
- Data acquisition, analysis and interpretation
- User training
- Advice on sample preparation
- User assistance and machine troubleshooting
- Help with design of experiments
- Sourcing of reagents and techniques; finding alternative approaches
- Help with data preparation for presentation and publication
Location
Room 303, 3rd Floor
Paul O'Gorman Building, UCL
72 Huntley Street, London, WC1E 6DD
Equipment
- Cell sorting (BD Aria and Fusion, and Miltenyi MACSQuant® Tyto)
- High throughput flow cytometry analysers (including small particle detection)
- Conventional flow cytometry analysers
- Laminar 96 well plate washer
Services
- Flow cytometry training, seminars and workshops
- Panel design/optimisation help and advice
- Experimental design/troubleshooting
- Basic and High Dimensional Data analysis support
- FlowJo and Cytobank software
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