Flow Cytometry

Guidelines for the use of the facility are provided on these web pages. Please ensure you have read the relevant guidelines to maximise the success and validity of your experiment.

How to get access

Location

Room X-1096, Lower Ground, Zayed Centre for Research into Rare Disease in Children (ZCR), 20 Guilford Street, London WC1N 1DZ.

Opening hours

Weekdays: Open 7–11pm. Core hours and most support services end at 6:30pm.
Weekends: Open 9am-9pm.
Bank Holidays: Open 9am-2pm with limited support services.

Contact

For enquiries, contact the Facility Manager, Dr Ayad Eddaoudi via email at a.eddaoudi@ucl.ac.uk or call (+44) 020 3978 3602.

Services available

The facility offers a range of services including:

cell sorting
cloning: single cell sorting
data acquisition, analysis and interpretation
user training
advice on sample preparation
user assistance and machine troubleshooting
help with design of experiments
sourcing of reagents and techniques; finding alternative approaches
help with data preparation for presentation and publication

We are also open to collaboration.

Equipment

The facility is equipped with state-of-the-art flow cytometry analysers / cell sorters.

Book an instrument or register

PPMS users only: Book analysers directly or request sorter access via email/phone. Check sorter availability below..

Book now

Only PPMS registered users have access to instruments and analysis computers. The facility has two types of instruments and two free-of-charge analysis computers. The instruments are either cell analysers or cell sorters. Users can directly book any cell analyser they trained on. Cell sorters are only operated and booked by the facility's staff. Users can check the availability of the cell sorters in the link above and call or send an email to the facility's staff indicating the time they would like to book their sort.

Flow Cytometers availability and booking

Name	Availability and booking
IR Symphony A5	Can be booked on the day/week, but book at least one week in advance for a convenient time.
Fisher Symphony A5	Can be booked on the day/week, but book at least one week in advance for a convenient time.
LSRII	Can be booked on the day/week.
CytoFLEX S	Quite busy: Book at least two weeks in advance for convenient time.
FACSAria III and FACSymphony S6 Cell Sorters	You are advised to book at least 2 weeks in advance for a convenient time slot. You can only book by sending an email to facility's staff. You can check availability from this website, go to home page and click on Check availability of a Cell Sorter.
FACSMelody Cell Sorter	Can be booked on the day/week.

Check Cell Sorter availability*

*must log in with flowcytometryich@googlemail.com account - requires PW that is available at the facility. You can only see availability.

Check availability

Charges and conditions of use

FlouroFinder: build your FCM panels

BenchSci: Search published antibody data

Training

Join Flow Club or Flow Clinic to build skills, troubleshoot data, and stay up to date with flow cytometry best practices.

Flow Club

Flow Club runs quarterly and is open to anyone who engages in flow cytometry.
Flow Club's main goal is to support good experimental practice for all users by coming together, solving problems, and sharing experiences.
Sessions include technical talks by facilities' users, tutorials about new technologies and equipment, and invited speakers.

Flow Clinic

Flow Clinic is an ad hoc workshop where users are welcome to bring their problem data to troubleshoot and clarify any issues with the help of the facility's staff.

For enquiries, contact the Facility Manager, Dr Ayad Eddaoudi via email at a.eddaoudi@ucl.ac.uk or call (+44) 020 3978 3602.

Publications

Michelozzi IM, Gomez-Castaneda E, Pohle RVC, Cardoso Rodriguez F, Sufi J, Puigdevall Costa P, Subramaniyam M, Kirtsios E, Eddaoudi A, Wu SW, Guvenel A, Fisher J, Ghorashian S, Pule MA, Tape CJ, Castellano S, Amrolia PJ, Giustacchini A. (2023) Activation priming and cytokine polyfunctionality modulate the enhanced functionality of low-affinity CD19 CAR T cells. Blood Adv. 2023 May 9;7(9):1725-1738. doi: 10.1182/bloodadvances.2022008490.PMID: 36453632
Ottaviano G, Georgiadis C, Gkazi SA, Syed F, Zhan H, Etuk A, Preece R, Chu J, Kubat A, Adams S, Veys P, Vora A, Rao K, Qasim W; TT52 CRISPR-CAR group.(2022) Phase 1 clinical trial of CRISPR-engineered CAR19 universal T cells for treatment of children with refractory B cell leukemia. Sci Transl Med. 2022 Oct 26;14(668):eabq3010. doi: 10.1126/scitranslmed.abq3010. Epub 2022 Oct 26.PMID: 36288281
Innes JA, Lowe AS, Fonseca R, Aley N, El-Hassan T, Constantinou M, Lau J, Eddaoudi A, Marino S, Brandner S. (2022) Phenotyping clonal populations of glioma stem cell reveals a high degree of plasticity in response to changes of microenvironment. Lab Invest. 2022 Feb;102(2):172-184. doi: 10.1038/s41374-021-00695-2. Epub 2021 Nov 15.PMID: 34782726
Shangaris P, Loukogeorgakis SP, Subramaniam S, Flouri C, Jackson LH, Wang W, Blundell MP, Liu S, Eaton S, Bakhamis N, Ramachandra DL, Maghsoudlou P, Urbani L, Waddington SN, Eddaoudi A, Archer J, Antoniou MN, Stuckey DJ, Schmidt M, Thrasher AJ, Ryan TM, De Coppi P, David AL. (2019). In Utero Gene Therapy (IUGT) Using GLOBE Lentiviral Vector Phenotypically Corrects the Heterozygous Humanised Mouse Model and Its Progress Can Be Monitored Using MRI Techniques. Sci Rep. 2019 Aug 12;9(1):11592. doi: 10.1038/s41598-019-48078-4.PMID: 31406195
Zelmer A, Stockdale L, Prabowo SA, Cia F, Spink N, Gibb M, Eddaoudi A, Fletcher HA. (2018). High monocyte to lymphocyte ratio is associated with impaired protection after subcutaneous administration of BCG in a mouse model of tuberculosis. Version 2. F1000Res. 2018 Mar 8 [revised 2018 Jun 27];7:296. doi: 10.12688/f1000research.14239.2. eCollection 2018.
Robertson A, Issitt R, Crook R, Gustafsson K, Eddaoudi A, Tsang V, Burch M.J Heart(2018). A novel method for ABO-incompatible heart transplantation. Lung Transplant. 2018 Apr;37(4):451-457. doi: 10.1016/j.healun.2017.05.006. Epub 2017 May 5.PMID: 28554587
Eddaoudi A, Canning SL, Kato I. (2018). Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining. Methods Mol Biol. 1686:49-57.
Boldrin L, Ross JA, Whitmore C, Doreste B, Beaver C, Eddaoudi A, Pearce DJ, Morgan JE. (2017). The effect of calorie restriction on mouse skeletal muscle is sex, strain and time-dependent. Sci Rep. 11;7(1):5160.
Robertson A, Issitt R, Crook R, Gustafsson K, Eddaoudi A, Tsang V, Burch M (2017). A novel method for ABO-incompatible heart transplantation. J Heart Lung Transplant. 2017 May 5.
Polycarpou A, Holland MJ, Karageorgiou I, Eddaoudi A, Walker SL, Willcocks S, Lockwood DN (2016). Mycobacterium leprae Activates Toll-Like Receptor-4 Signaling and Expression on Macrophages Depending on Previous Bacillus Calmette-Guerin Vaccination. Front Cell Infect Microbiol. 2016 Jul 8;6:72.
Weng A, Manunta MD, Thakur M, Gilabert-Oriol R, Tagalakis AD, Eddaoudi A, Munye MM, Vink CA, Wiesner B, Eichhorst J, Melzig MF, Hart SL (2015). Improved intracellular delivery of peptide- and lipid-nanoplexes by natural glycosides. J Control Release. 2015 Mar 7.
Stevens E, Torelli S, Feng L, Phadke R, Walter MC, Schneiderat P, Eddaoudi A, Sewry CA, Muntoni F (2013). Flow Cytometry for the Analysis of α-Dystroglycan Glycosylation in Fibroblasts from Patients with Dystroglycanopathies. PLoS One. 2013 Jul 22;8(7)
Moschidou D, Drews K, Eddaoudi A, Adjaye J, De Coppi P, Guillot PV (2013). Molecular signature of human amniotic fluid stem cells during fetal development. Curr Stem Cell Res Ther. 8(1):73-81.
Chan E, Schaller T, Eddaoudi A, Zhan H, Tan CP, Jacobsen M, Thrasher A, Towers GJ, Qasim W. (2012). Lentiviral gene therapy against HIV-1 using a novel human TRIM21-CyclophilinA restriction factor. Hum Gene Ther. 23(11):1176-85
Dong R, Moulding D, Himoudi N, Adams S, Bouma G, Eddaoudi A, Basu BP, Derniame S, Chana P, Duncan A, Anderson J (2011). Cells with dendritic cell morphology and immunophenotype, binuclear morphology, and immunosuppressive function in dendritic cell cultures. Cell Immunol. 272(1):1-10.
Ali-Boucetta H, Al-Jamal KT, Müller KH, Li S, Porter AE, Eddaoudi A, Prato M, Bianco A, Kostarelos K (2011). Cellular Uptake and Cytotoxic Impact of Chemically Functionalized and Polymer-Coated Carbon Nanotubes. Small. 7(22):3230-8. Al-Jamal KT, Toma FM, Yilmazer A, Ali-Boucetta H, Nunes A, Herrero MA, Tian B, Eddaoudi A, Al-Jamal WT, Bianco A, Prato M, Kostarelo K (2010). Enhanced cellular internalization and gene silencing with a series of cationic dendron-multiwalled carbon nanotube:siRNA complexes. FASEB J. 24(11):4354-65.
Eddaoudi A, Townsend-Nicholson A, Timms JF, Schorged S, Jayasinghe SN (2010). Molecular characterisation of post-bio-electrosprayed human brain astrocytoma cells. Analyst. 135(10):2600-12.
Joly P, Chavda N, Eddaoudi A, Jayasinghe SN (2010) Bio-electrospraying and aerodynamically assisted bio-jetting whole human blood: Interrogating cell surface marker integrity. Biomicrofluidics. 4(1): 011101-6.
Moncrieffe H, Nistala K, Kamhieh Y, Evans J, Eddaoudi A, Eaton S, Wedderburn LR (2010) High expression of the ectonucleotidase CD39 on T cells from the inflamed site identifies two distinct populations, one regulatory and one memory T cell population. J Immunol. 185(1): 134-43.
Hunter PJ, Nistala K, Jina N, Eddaoudi A, Thomson W, Hubank M, Wedderburn LR (2010) Biologic predictors of extension of oligoarticular juvenile idiopathic arthritis as determined from synovial fluid cellular composition and gene expression. Arthritis Rheum. 62(3): 896-907.
Himoudi N, Yan M, Bouma G, Morgenstern D, Wallace R, Seddon B, Buddle J, Eddaoudi A, Howe SJ, Cooper N, Anderson J (2009) Migratory and Antigen Presentation Functions of IFN-Producing Killer Dendritic Cells. Cancer Res. 69(16) 6598-6606.
Loebinger MR, Eddaoudi A, Davies D, Janes SM (2009) Mesenchymal stem cell delivery of TRAIL can eliminate metastatic cancer. Cancer Res. 69(10): 4134-42.
Blair PA, Chavez-Rueda KA, Evans JG, Shlomchik MJ, Eddaoudi A, Isenberg DA, Ehrenstein MR, Mauri C (2009) Selective targeting of B cells with agonistic anti-CD40 is an efficacious strategy for the generation of induced regulatory T2-like B cells and for the suppression of lupus in MRL/lpr mice. J Immunol 182(6): 3492-502.
Himoudi N, Nabarro S, Buddle J, Eddaoudi A, Thrasher AJ, Anderson J (2008).Bone marrow-derived IFN-producing killer dendritic cells account for the tumoricidal activity of unpulsed dendritic cells. J Immunol 181(9): 6654-63.

Piper CJM, Wilkinson MGL, Deakin CT, Otto GW, Dowle S, Duurland CL, Adams S, Marasco E, Rosser EC, Radziszewska A, Carsetti R, Ioannou Y, Beales PL, Kelberman D, Isenberg DA, Mauri C, Nistala K, Wedderburn LR. 2018. CD19+CD24hiCD38hi B Cells Are Expanded in Juvenile Dermatomyositis and Exhibit a Pro-Inflammatory Phenotype After Activation Through Toll-Like Receptor 7 and Interferon-α. Front Immunol. Jun 22;9:1372
Payne H, Chain G, Adams S, Hunter P, Luckhurst N, Gilmour K, Lewis J, Babiker A, Cotton M, Violari A, Gibb D, Callard R, Klein N. 2018. Naïve B-cell output in HIV-infected and HIV-uninfected children. AIDS Res Hum Retroviruses.
Nowak K, Linzner D, Thrasher AJ, Lambert PF, Di WL, Burns SO. 2017 Absence of γ-Chain in Keratinocytes Alters Chemokine Secretion, Resulting in Reduced Immune Cell Recruitment. J Invest Dermatol. Oct; 137(10):2120-2130.
Mavroudis CA, Eleftheriou D, Hong Y, Majumder B, Koganti S, Sapsford R, North J, Lowdell M, Klein N, Brogan P, Rakhit RD. 2017. Microparticles in acute coronary syndrome. Thromb Res. Aug; 156:109-116.
Solanki A, Lau CI, Saldaña JI, Ross S, Crompton T. 2017. The transcription factor Gli3 promotes B cell development in fetal liver through repression of Shh. J Exp Med. Jul; 214(7):2041-2058.
Tagalakis AD, Maeshima R, Yu-Wai-Man C, Meng J, Syed F, Wu LP, Aldossary AM, McCarthy D, Moghimi SM, Hart SL. 2017. Peptide and nucleic acid-directed self-assembly of cationic nanovehicles through giant unilamellar vesicle modification: Targetable nanocomplexes for in vivo nucleic acid delivery. Acta Biomater. Mar; 51:351-362.
Liyanage SE, Gardner PJ, Ribeiro J, Cristante E, Sampson RD, Luhmann UF, Ali RR, Bainbridge JW. 2016. Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models. Exp Eye Res. Oct; 151:160-70.
Eisen S, Hayden C, Young CJ, Gilson R, Jungmann E, Jacobsen MC, Poulsom H, Goldblatt D, Klein NJ, Baxendale HE. 2016. B-cell development and pneumococcal immunity in vertically acquired HIV infection. AIDS. Jul; 30(12):1867-76.
Kwok A, McCarthy D, Hart SL, Tagalakis AD. 2016. Systematic Comparisons of Formulations of Linear Oligolysine Peptides with siRNA and Plasmid DNA. Chem Biol Drug Des May; 87(5):747-63.
Kanabus M, Fassone E, Hughes SD, Bilooei SF, Rutherford T, Donnell MO, Heales SJ, Rahman S. 2016. The pleiotropic effects of decanoic acid treatment on mitochondrial function in fibroblasts from patients with complex I deficient Leigh syndrome. J Inherit Metab Dis. May; 39(3):415-26.
Hunter PJ, Sheikh S, David AL, Peebles DM, Klein N. 2016. Cervical leukocytes and spontaneous preterm birth. J Reprod Immunol. Feb;113:42-9.
Bacchelli C, Moretti FA, Carmo M, Adams S, Stanescu HC, Pearce K, Madkaikar M, Gilmour KC, Nicholas AK, Woods CG, Kleta R, Beales PL, Qasim W, Gaspar HB. 2016. Mutations in linker for activation of T cells (LAT) lead to a novel form of severe combined immunodeficiency. J Allergy Clin Immunol. J Allergy Clin Immunol. Feb;139(2):634-642.
Hiwarkar P, Qasim W, Ricciardelli I, Gilmour K, Quezada S, Saudemont A, Amrolia P, Veys P. 2015. Cord blood T cells mediate enhanced antitumor effects compared with adult peripheral blood T cells. Blood. Dec;126(26):2882-91.
Georgiadis C, Syed F, Petrova A, Abdul-Wahab A, Lwin SM, Farzaneh F, Chan L, Ghani S, Fleck RA, Glover L, McMillan JR, Chen M, Thrasher AJ, McGrath JA, Di WL, Qasim W. 2015. Lentiviral Engineered Fibroblasts Expressing Codon Optimized COL7A1 Restore Anchoring Fibrils in RDEB. J Invest Dermatol. Sep 22.
Watson AS, Riffelmacher T, Stranks A, Williams O, De Boer J, Cain K, MacFarlane M, McGouran J, Kessler B, Khandwala S, Chowdhury O, Puleston D, Phadwal K, Mortensen M, Ferguson D, Soilleux E, Woll P, Jacobsen SE, Simon AK. 2015. Autophagy limits proliferation and glycolytic metabolism in acute myeloid leukemia. Cell Death Discov. Aug 17;1.
Binder E, Natarajan D, Cooper J, Kronfli R, Cananzi M, Delalande JM, McCann C, Burns AJ, Thapar N. 2015. Enteric neurospheres are not specific to neural crest cultures: implications for neural stem cell therapies. PLoS One. 10(3):e0119467.
Eleftheriou D, Ganesan V, Hong Y, Klein NJ, Brogan PA. 2015. Endothelial Repair in Childhood Arterial Ischaemic Stroke with Cerebral Arteriopathy. Cerebrovasc Dis Extra. Jun; 5(2):68-74
Chayka O, D'Acunto CW, Middleton O, Arab M, Sala A. 2015. Identification and pharmacological inactivation of the MYCN gene network as a therapeutic strategy for neuroblastic tumor cells. J Biol Chem. Jan; 290(4):2198-212.
Mock U, Riecken K, Berdien B, Qasim W, Chan E, Cathomen T, Fehse B. 2014. Novel lentiviral vectors with mutated reverse transcriptase for mRNA delivery of TALE nucleases. Sci Rep. Sep 18;4:6409.
Maghsoudlou P, Ditchfield D, Klepacka DH, Shangaris P, Urbani L, Loukogeorgakis SP, Eaton S, De Coppi P. 2014. Isolation of esophageal stem cells with potential for therapy. Pediatr Surg Int. 30(12):1249-56.
Warre-Cornish K, Barber AC, Sowden JC, Ali RR, Pearson RA. 2014. Migration, integration and maturation of photoreceptor precursors following transplantation in the mouse retina. Stem Cells Dev. 23(9):941-54.
Natarajan D, Cooper J, Choudhury S, Delalande JM, McCann C, Howe SJ, Thapar N, Burns AJ., 2014. Lentiviral labeling of mouse and human enteric nervous system stem cells for regenerative medicine studies. Neurogastroenterol Motil. 26(10):1513-8.
Osaki H, Walf-Vorderwülbecke V, Mangolini M, Zhao L, Horton SJ, Morrone G, Schuringa JJ, de Boer J, Williams O. 2013. The AAA+ ATPase RUVBL2 is a critical mediator of MLL-AF9 oncogenesis. Leukemia. Jul;27(7):1461-8.
Mangolini M, de Boer J, Walf-Vorderwülbecke V, Pieters R, den Boer ML, Williams O 2013. STAT3 mediates oncogenic addiction to TEL-AML1 in t(12;21) acute lymphoblastic leukemia. Blood. 2013 Jul 25;122(4):542-9.
Zhan H, Gilmour K, Chan L, Farzaneh F, McNicol AM, Xu JH, Adams S, Fehse B, Veys P, Thrasher A, Gaspar H, Qasim W., 2013. Production and first-in-man use of T cells engineered to express a HSVTK-CD34 sort-suicide gene. PLoS One. 8(10).
Andoniadou CL, Matsushima D, Mousavy Gharavy SN, Signore M, Mackintosh AI, Schaeffer M, Gaston-Massuet C, Mollard P, Jacques TS, Le Tissier P, Dattani MT, Pevny LH, Martinez-Barbera JP., 2013. Sox2(+) stem/progenitor cells in the adult mouse pituitary support organ homeostasis and have tumor-inducing potential. Cell Stem Cell. 13(4):433-45.
Field AC, Vink C, Gabriel R, Al-Subki R, Schmidt M, Goulden N, Stauss H, Thrasher A, Morris E, Qasim W. 2013. Comparison of lentiviral and sleeping beauty mediated αβ T cell receptor gene transfer. PLoS One. 2013 Jun 28;8(6).
Gonzalez-Cordero A, West EL, Pearson RA, Duran Y, Carvalho LS, Chu CJ, Naeem A, Blackford SJ, Georgiadis A, Lakowski J, Hubank M, Smith AJ,Bainbridge JW, Sowden JC, Ali RR., 2013. Photoreceptor precursors derived from three-dimensional embryonic stem cell cultures integrate and mature within adult degenerate retina. Nat Biotechnol. 31(8):741-7.
Stevens E, Carss KJ, Cirak S, Foley AR, Torelli S, Willer T, Tambunan DE, Yau S, Brodd L, Sewry CA, Feng L, Haliloglu G, Orhan D, Dobyns WB, Enns GM,Manning M, Krause A, Salih MA, Walsh CA, Hurles M, Campbell KP, Manzini MC; UK10K Consortium, Stemple D, Lin YY, Muntoni F., 2013. Mutations in B3GALNT2 cause congenital muscular dystrophy and hypoglycosylation of α-dystroglycan. Am J Hum Genet. 92(3):354-65.
Pesenacker AM, Bending D, Ursu S, Wu Q, Nistala K, Wedderburn LR. 2013. CD161 defines the subset of FoxP3+ T cells capable of producing proinflammatory cytokines. Blood. 121(14):2647-58.
Albon SJ, Mancao C, Gilmour K, White G, Ricciardelli I, Brewin J, Lugthart G, Wallace R, Amrolia PJ., 2013. Optimization of methodology for production of CD25/CD71 allodepleted donor T cells for clinical use. Cytotherapy. 15(1):109-21.
Barber AC, Hippert C, Duran Y, West EL, Bainbridge JW, Warre-Cornish K, Luhmann UF, Lakowski J, Sowden JC, Ali RR, Pearson RA., 2013. Repair of the degenerate retina by photoreceptor transplantation. Proc Natl Acad Sci U S A. 110(1):354-9.
Medjkane S, Perichon M, Marsolier J, Dairou J, Weitzman JB., 2013. Theileria induces oxidative stress and HIF1α activation that are essential for host leukocyte transformation. Oncogene. doi: 10.1038/onc.2013.134.
Walf-Vorderwülbecke V, de Boer J, Horton SJ, van Amerongen R, Proost N, Berns A, Williams O (2012). Frat2 mediates the oncogenic activation of Rac by MLL fusions. Blood. 120(24):4819-28
Kueh JL, Li D, Raisman G, Jenkins D, Li Y, Stevens R. 2012. Directionality and bipolarity of olfactory ensheathing cells on electrospun nanofibers. Nanomedicine (Lond). Aug;7(8):1211-24.
West EL, Gonzalez-Cordero A, Hippert C, Osakada F, Martinez-Barbera JP, Pearson RA, Sowden JC, Takahashi M, Ali RR., 2012. Defining the integration capacity of embryonic stem cell-derived photoreceptor precursors. Stem Cells. 30(7):1424-35.
Jayakody SA, Andoniadou CL, Gaston-Massuet C, Signore M, Cariboni A, Bouloux PM, Le Tissier P, Pevny LH, Dattani MT, Martinez-Barbera JP., 2012 . SOX2 regulates the hypothalamic-pituitary axis at multiple levels. J Clin Invest. 122(10):3635-46.
Zibara K, Hamdan R, Dib L, Sindet-Pedersen S, Kharfan-Dabaja M, Bazarbachi A, El-Sabban M. 2012. Acellular bone marrow extracts significantly enhance engraftment levels of human hematopoietic stem cells in mouse xeno-transplantation models. PLoS One. 2012;7(7):e40140. doi: 10.13.
Guasti L, Prasongchean W, Kleftouris G, Mukherjee S, Thrasher AJ, Bulstrode NW, Ferretti P., 2012. High plasticity of pediatric adipose tissue-derived stem cells: too much for selective skeletogenic differentiation? Stem Cells Transl Med. 1(5):384-95.
Eleftheriou D, Ganesan V, Hong Y, Klein NJ, Brogan PA., 2012. Endothelial injury in childhood stroke with cerebral arteriopathy: a cross-sectional study. Neurology. 79(21):2089-96.
Andoniadou CL, Gaston-Massuet C, Reddy R, Schneider RP, Blasco MA, Le Tissier P, Jacques TS, Pevny LH, Dattani MT, Martinez-Barbera JP., 2012. Identification of novel pathways involved in the pathogenesis of human adamantinomatous craniopharyngioma. Acta Neuropathol. 124(2):259-71.
Lau CI, Outram SV, Saldaña JI, Furmanski AL, Dessens JT, Crompton T., 2012. Regulation of murine normal and stress-induced erythropoiesis by Desert Hedgehog (Dhh). Blood. May 17;119(20):4741-51.
Pearson RA, Barber AC, Rizzi M, Hippert C, Xue T, West EL, Duran Y, Smith AJ, Chuang JZ, Azam SA, Luhmann UF, Benucci A, Sung CH, Bainbridge JW, Carandini M, Yau KW, Sowden JC, Ali RR., 2012 . Restoration of vision after transplantation of photoreceptors. Nature. 485(7396):99-103.
Furmanski AL, Saldana JI, Rowbotham NJ, Ross SE, Crompton T., 2012. Role of Hedgehog signalling at the transition from double-positive to single-positive thymocyte. Eur J Immunol. 42(2):489-99.
Montiel-Equihua CA, Zhang L, Knight S, Saadeh H, Scholz S, Carmo M, Alonso-Ferrero ME, Blundell MP, Monkeviciute A, Schulz R, Collins M, Takeuchi Y, Schmidt M, Fairbanks L, Antoniou M, Thrasher AJ, Gaspar HB., 2012. The β-Globin Locus Control Region in Combination With the EF1α Short Promoter Allows Enhanced Lentiviral Vector-mediated Erythroid Gene Expression With Conserved Multilineage Activity. Mol Ther.
Himoudi N, Morgenstern DA, Yan M, Vernay B, Saraiva L, Wu Y, Cohen CJ, Gustafsson K, Anderson J., 2012. Human γδ T Lymphocytes Are Licensed for Professional Antigen Presentation by Interaction with Opsonized Target Cells. J Immunol. 188(4):1708-16.
Kim J, Jimenez-Mallebrera C, Foley AR, Fernandez-Fuente M, Brown SC, Torelli S, Feng L, Sewry CA, Muntoni F., 2012. Flow cytometry analysis: A quantitative method for collagen VI deficiency screening. Neuromuscul Disord. 22(2):139-48.
Di WL, Semenova E, Larcher F, Del Rio M, Harper JI, Thrasher AJ, Qasim W. 2012. Human involucrin promoter mediates repression-resistant and compartment-specific LEKTI expression. Hum Gene Ther. Jan;23(1):83-90. doi: 10.1089/hum.2011.091.
Kueh JL, Raisman G, Li Y, Stevens R, Li D. 2011. Comparison of bulbar and mucosal olfactory ensheathing cells using FACS and simultaneous antigenic bivariate cell cycle analysis. Glia. 2011 Nov;59(11):1658-71.
Pinheiro D, Singh Y, Grant CR, Appleton RC, Sacchini F, Walker KR, Chadbourne AH, Palmer CA, Armitage-Chan E, Thompson I, Williamson L, Cunningham F, Garden OA. 2011. Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog. Immunology. 132(1):111-22.
Furmanski AL, Saldana JI, Rowbotham NJ, Ross SE, Crompton T., 2011. Role of Hedgehog signalling at the transition from double-positive to single-positive thymocyte. Eur J Immunol. 42(2):489-99
Lakowski J, Han YT, Pearson RA, Gonzalez-Cordero A, West EL, Gualdoni S, Barber AC, Hubank M, Ali RR, Sowden JC., 2011. Effective transplantation of photoreceptor precursor cells selected via cell surface antigen expression. Stem Cells. 29(9):1391-404.
de Boer J, Yeung J, Ellu J, Ramanujachar R, Bornhauser B, Solarska O, Hubank M, Williams O, Brady HJ. 2011. The E2A-HLF oncogenic fusion protein acts through Lmo2 and Bcl-2 to immortalize hematopoietic progenitors. Leukemia. Feb;25(2):321-30.
Andoniadou CL, Signore M, Young RM, Gaston-Massuet C, Wilson SW, Fuchs E, Martinez-Barbera JP, 2011. HESX1- and TCF3-mediated repression of Wnt/β-catenin targets is required for normal development of the anterior forebrain. Development. 138(22):4931-42.
Edwards LA, Nistala K, Mills DC, Stephenson HN, Zilbauer M, Wren BW, Dorrell N, Lindley KJ, Wedderburn LR, Bajaj-Elliott M., 2011. Delineation of the innate and adaptive T-cell immune outcome in the human host in response to Campylobacter jejuni infection. PLoS One. 5(11):e15398.
Qasim W, Derniame S, Gilmour K, Chiesa R, Weber M, Adams S, Rao K, Amrolia P, Goulden N, Veys P, Gaspar H. 2011. Third-party virus-specific T cells eradicate adenoviraemia but trigger bystander graft-versus-host disease. Br J Haematol. Jul;154(1):150-3.
Yan M, Himoudi N, Basu BP, Wallace R, Poon E, Adams S, Hasan F, Xue SA, Wilson N, Dalgleish A, Williams O, Anderson J, 2011. Increased PRAME antigen-specific killing of malignant cell lines by low avidity CTL clones, following treatment with 5-Aza-2'-Deoxycytidine. Cancer Immunol Immunother. 60(9):1243-55.
Smart N, Bollini S, Dubé KN, Vieira JM, Zhou B, Davidson S, Yellon D, Riegler J, Price AN, Lythgoe MF, Pu WT, Riley PR, 2011. De novo cardiomyocytes from within the activated adult heart after injury. Nature. 474(7353):640-4.
Meng J, Adkin CF, Xu SW, Muntoni F, Morgan JE., 2011. Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice. PLoS One. 6(3):e17454.
Bollini S, Pozzobon M, Nobles M, Riegler J, Dong X, Piccoli M, Chiavegato A, Price AN, Ghionzoli M, Cheung KK, Cabrelle A, O'Mahoney PR, Cozzi E, Sartore S, Tinker A, Lythgoe MF, De Coppi P, 2011. In vitro and in vivo cardiomyogenic differentiation of amniotic fluid stem cells. Stem Cell Rev. 7(2):364-80.
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Funding and acknowledgements

Please acknowledge use of the facility in all publications. Suggested text: Flow cytometry was performed at the UCL Great Ormond Street Institute of Child Health, supported by the Great Ormond Street Children's Charity (GOSHCC), seed grant reference U09822 (October 2007), UCL Capital Equipment Funding, School of Life and Medical Sciences (September 2012; February 2022).

All research at Great Ormond Street Hospital NHS Foundation Trust and UCL Great Ormond Street Institute of Child Health is made possible by the NIHR Great Ormond Street Hospital Biomedical Research Centre. The views expressed are those of the author(s) and not necessarily those of the NHS, the NIHR or the Department of Health.