IPLS Seminar - Prof. Sergi Garcia-Manyes (Kings College London)

Abstract: The translocation of mechanosensitive transcription factors (TFs) across the nuclear envelope is a crucial step in cellular mechanotransduction. Yet the molecular mechanisms by which external mechanical cues control the nuclear shuttling dynamics of TFs through the nuclear pore complex (NPC) to activate gene expression are poorly understood. Within a multiscale experimental approach, using a combination of single-molecule force spectroscopy, single-cell mechanics, live-cell imaging and cellular-motility assays, here we show that the nuclear import rate of myocardin-related transcription factor A (MRTFA) — a protein that regulates cytoskeletal dynamics via the activation of the TF serum response factor (SRF) — inversely correlates with the protein’s mechanical stability and does not relate to its thermodynamic stability. Tagging MRTFA with mechanically resistant proteins results in the downregulation of SRF-mediated myosin light-chain 9 (MYL9) gene expression and subsequent slowing down of cell migration. We conclude that the mechanical unfolding of proteins regulates their nuclear translocation rate through the NPC, and highlight the role of the NPC as a selective mechanosensor able to discriminate forces as low as ~10 pN. The modulation of the mechanical stability of TFs may represent a new strategy for the control of gene expression.