Creoptix WAVEsystem

The WAVE puts binding kinetics analysis at your fingertips. Its high data quality, sample compatibility and automated software enables drug discovery and inroads into R&D. For UCL and external users.

This GCI system is equivalent but more sensitive than surface plasmon resonance (SPR, Biacore).  Essentially a lab on a chip system it can determine interactions of biomolecules to high precision and also determine the kinetics of the interactions. 

How to get access

Location

Cruciform Building, Bloomsbury

Available to

UCL and External Users

Prices

£100 per day. £1,500 per year for unlimited access.

Bookings

For more information on how to make a booking

Find out more

Contact

Reuben Asher

Reuben Asher

Lab Manager

Click to email. reuben.asher.20@ucl.ac.uk
UCL Cruciform Building

Primary Specifications

  • Crude samples, harsh chemicals, and particles up to 1000 nm
  • Self-contained disposable cartridge and small footprint
  • Ultra-fast transition times of 150 ms with reliable determination of off-rates up to 10 s-1
  • Superior signal-to-noise ratios (0.01 pg/mm2 at 1 Hz)
  • Reliable kinetics and binding affinities (KD) from low pM to low mM with from signals below 1 pg/mm2
  • Analyze large ligand to analyte molecular weight ratios up to >1000:1
  • Up to 120 hours of unattended operation
Creoptix WAVE GCI/SPR (Credit: Creoptix / Malvern Panalytical)

Detailed specifications

Breakthrough Binding Kinetics Analysis

  • Expands affinity range and sample compatibility.
  • Enhances drug discovery and R&D through high data quality and sophisticated software.

Real-Time Label-Free Binding Kinetics

  • Combines robustness for crude samples, high sensitivity, and advanced software.
  • Innovative microfluidics and proprietary Grating-Coupled Interferometry (GCI) technology.

High Sensitivity

  • Superior to SPR in reading refractive index changes, leading to higher sensitivity.
  • Waveguide’s evanescent field minimizes bulk disturbances, ensuring high signal-to-noise ratios.
  • Measures kinetics (ka, kd) and affinity (KD) from low pM to low mM, even from very low signals (<1RU).

Temperature-Controlled Autosampler

  • Can handle various sample formats (vial racks, well plates).
  • Allows sensitivity with low immobilization levels and large ligand-to-analyte MW ratios.

Large Ligand-to-Analyte Molecular Weight Ratios

  • Suitable for large target-to-analyte molecular weight ratios up to >1000:1.
  • Ensures accurate kinetic analysis of low-potency small molecules or targets with low activity.

More Signal, Less Noise

  • GCI’s evanescent field reduces bulk refractive index disturbances.
  • Results in superior signal-to-noise ratios.

Robust Readout

  • Time-domain interference readout within the waveguide offers robustness.
  • Time-resolved phase-shift signal measurement provides superior resolution despite temperature drifts or vibrations.

Bookings

All UCL staff and students can book this equipment through Outlook. Bookings are made by the hour starting on the hour.

Please contact Reuben Asher (reuben.asher.20@ucl.ac.uk) for details.

Once you are registered as a user, please provide the following mandatory information when you book.

Internal bookings

  • Full name
  • Department
  • Your Finance Officer’s name
  • Account code for charges
  • The equipment you wish to use
  • Hours / dates required

External bookings

  • Full name
  • Company address and department name
  • Finance contact details: address, telephone number and email
  • PO number
  • PO Description: equipment name, date and hours booked
  • VAT number
  • Amount
Image credit

Creoptix / Malvern Panalytical