Immune Monitoring and Discovery Core
The core facility provides the tools to comprehensively evaluate patient immune responses through progression and therapy.
The aim of this core facility is to provide all of the tools necessary for comprehensive evaluation of patient immune responses through progression and therapy. This will maximise the potential of participating clinical trials by allowing the clinical samples obtained to be fully evaluated.
Our facilities and services can help support clinical trials from sample collection, processing and storage. We can also provide immune monitoring and functional analysis.
IMDC Facilities
We will create a hub of expertise for researchers and clinicians with access to state-of-the-art facilities and equipment for:
- Multi-parameter flow and mass cytometry
- Multiplex immunohistochemistry (including RNAscope and FDA approved Ventana PD-L1 assays)
- Genomics
- Proteomics (including mass pectrometry and targeted protein profiling)
- Recombinant Protein Production
- Bioinformatics.
We can also arrange collaborations with members of our Network to access their expertise and functional assays developed in their labs.
For more details please contact ci.cita@ucl.ac.uk.
IMDC Equipment
- BD FACS Fortessa cell sorter and Fusion Aria cell sorter in place in the Flow Cytometry Core Facility with Prime Flow from Affymetrix/eBoscience (for detecting RNA and Protein simultaneously)
- FACS Verse available in the GCLP lab
- Helios Mass Cytometer
- Vectra (Multiplex histopathology imaging system) in place in the Microscopy and Imaging Core Facility
- Leica Bond Rxm for automated RNAScope and BaseScope (multiplex in situ hybridisation)
- Thermo Fisher Q-exactive Plus and Easy LC Mass spectrometer in place in the Proteomics Core Research Facility
- GE Wave Bioreactor
- MaxCyte STX cell electroporator (Scalable high efficiency transfection using transient gene experession) for biotherapeutic discovery and development
in place in the Recombinant Protein lab - Nanostring nCounter Sprint for performing multianalyte measurement of both RNA and proteins simultaneously with single tube multiplexing.
