Mass Spectrometry Instrumentation

The facility provides nominal and accurate mass measurements using the equipment detailed below.

Ion generation: heated electrospray ionisation (HESI), atmospheric pressure chemical ionisation (APCI), atmospheric solids analysis probe (ASAP)
Mass range: m/z 50 to 6,000
Mass resolution: at m/z 200 is 35,000 and at m/z 400 is 25,000 at scan rate 7 Hz
Mass accuracy: internal <1 ppm RMS, external <3 ppm RMS under defined conditions
Dissociation: in-source CID, HCD
Scan functions: Full scan (FS), all ion fragmentation (AIF), SIM, parallel reaction monitoring (PRM), data dependent acquisition (DIA), data dependent HCD (ddHCD)
Simultaneous polarity switching: one full cycle in <1 sec (one full scan positive mode and one full scan negative mode at resolution setting of 35,000)during LC-MS run
Intact Protein Analysis: unit resolution of Ubiquitin (8 kDa)
Sample introduction through:- (a) direct infusion through a syringe connected to the pump or (b) Vanquisher Liquid Chromatograph with an autosampler and degasser.
Xcalibur software for method setup, data processing, and reporting.
Thermo Scientific FreeStyle software to view and analyse data

Ion generation: capillary ESI
Mass range: m/z 50 to 20,000
Mass resolution: m/z 100 to 3,200 in MS mode while maintaining a resolution of 13,000 at m/z 2,722
Mass accuracy: lock mass calibration at set m/z ions of calibration mixture less than 2 ppm
Dissociation: CID
Scan functions: full scan (MS), MS/MS, data dependent acquisition
Simultaneous polarity switching: during one LC-MS run not possible, either runs in negative or positive ESI mode
Intact protein analysis: unit resolution of Ubiquitin (8 kDa)
Sample introduction through: (a) direct infusion through a syringe connected to the pump or (b) Agilent 1200 Liquid Chromatograph with an autosampler and degasser.
MassHunter Software for method setup, data processing, and reporting

Ion generation: Electron ionisation (EI), Chemical ionisation (CI) with ammonia or methane
Mass range: m/z 50 to 1,000
Mass resolution: one unit resolution
Mass accuracy: ±0.5 Da
Dissociation: EI fragmentation
Scan functions: Full scan (FS), single ion monitoring (SIM)
Simultaneous polarity switching: not possible
Small molecules analysis: volatile, semi-volatile, PAH, steroids and etc.
Incubation oven is available up to 200˚C
Sample introduction through:- (a) liquid injections; (b) gases injection through a head-space vials, (c) solid phase micro-extraction (SPME) (d) direct injection of the sample into the GC inlet.
Xcalibur software for method setup, data processing, and reporting

Ion generation: electrospray ionisation (ESI), atmospheric pressure chemical ionisation (APCI)
Mass range: m/z 50 to 2,000
Mass resolution: unit resolution
Mass accuracy: ±0.5 Da
Scan functions: Full scan (FS), Selected Reaction Monitoring (SRM), MSn for multi-stage MS experiments, data dependent acquisition (DIA), ZoomScan for ultimate resolution.
Simultaneous polarity switching: not possible
Intact Protein Analysis: unit resolution of Ubiquitin (8 kDa)
Sample introduction through:- (a) direct infusion through a syringe connected to the pump or (b) Accela Liquid Chromatograph with an autosampler.
Xcalibur software for method setup, data processing, and reporting

Ion generation: matrix-assisted laser desorption (MALDI)
Mass range: 1 to 350,000 Da and 10,000 to 500,00 Da
Modes: Linear; positive and negative polarities
Laser: 200 Hz solid-state laser, 355 nm
Mass resolution: >5,000 FWHM (full width half-height maximum) on ACTH 18-29 (2,465 Da)
Mass accuracy: <200 ppm with internal calibration, <150 ppm with external calibration (nearest neighbour external calibration on fleximass-SR48 steel target)
Sensitivity: 250 fmol on bovine serum albumin, 250 amol on Glu-1-Fibrinopeptide B
Sample introduction: by mixing matrix with sample and laying on the MALDI plate.
Shimadzu software for method setup, data processing, and reporting

Ion generation: electrospray ionisation (ESI), atmospheric pressure chemical ionisation (APCI)
Mass range: m/z 50 to 2,000
Mass resolution: unit resolution
Mass accuracy: ±0.5 Da
Scan functions: Full scan (FS), Selected Reaction Monitoring (SRM).
Simultaneous polarity switching: possible
Intact Protein Analysis: unit resolution of Ubiquitin (8 kDa)
Sample introduction through: (a) direct infusion through a syringe connected to the pump or (b) UPLC Liquid Chromatograph with an autosampler and degasser.
MassLynx software for method setup, data processing, and reporting

Ion generation: electrospray ionisation (ESI)
Mass range: m/z 50 to 6,000
Mass resolution: at m/z 200 is 15,000
Mass accuracy: less than 5 ppm external calibration and less than 2 ppm lock-mass calibration
Dissociation: not possible
Scan functions: Full scan (FS).
Simultaneous polarity switching: negative and positive modes
Sample introduction through:- (a) direct infusion through a syringe connected to the pump or (b) an autosampler
MassLynx software for method setup, data processing, and reporting

Ion generation: electrospray ionisation (ESI)
Mass range: m/z 50 to 3,000
Mass resolution: unit resolution
Mass accuracy: ±0.5 Da
Scan functions: Full scan (FS), Selected Reaction Monitoring (SRM).
Simultaneous polarity switching: possible
Intact Protein Analysis: unit resolution of Ubiquitin (8 kDa)
Sample introduction through: UPLC Liquid Chromatograph with an autosampler and degasser.
MassLynx software for method setup, data processing, and reporting