Information for module BIOC1007
This module is available for: The current academic year
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|Module code:||BIOC1007(Add to my personalised list)|
|Title:||The Principles and Practice of Experimental Biochemistry|
|Division:||Division of Biosciences|
|Module organiser:||Prof P Rich|
|Organiser's location:||Darwin Building|
|Available for students in Year(s):||1,|
|Module prerequisites:||Available only to students registered on BSc Biochemistry and BSc Biotechnology|
|Module outline:||TERM 1 L1: Introduction to the course L2: Introduction to basic mathematics in the course L3: pH, pKa, buffers and equilibria L4: Free energy, redox potentials, pH electrodes L5-6: Spectrometry L7-9: Introduction to amino acids and sequencing L10-12: Basics of protein sequences and structures L13-15: Methods for determining protein structure TERM 2 L16-18: Design and interpretation of experiments L17-18: Statistics L19-21: Purifying proteins L22-24: Applications of Molecular Biology L25-28: Pathways and cycles L29-30: Use of isotopes|
|Module aims:||Introduction to techniques and approaches that are used to investigate biochemical problems, together with their underlying principles. It is also designed to give experience in practical work, calculations and reading scientific papers.. Specific areas are pH, redox potentials and equilibrium; basic spectroscopy; amino acids, protein sequences and structures; mass spec., NMR and X-ray methods; statistical methods; protein purification and assays; molecular biology methods; metabolic pathways; uses of isotopes in biochemistry.|
|Module objectives:||• be able to make straightforward biochemical calculations, at a level of those included in the tutorial exercises • understand pH, buffers, chemical equilibrium, exergonic and endergonic reactions, acid dissociation constant (pKa) and redox potentials • understand the basics of UV/visible and fluorescence spectroscopy and their applications in biochemistry • become familiar with the amino acid components of proteins, how they link together and how you can determine amino acid sequences of proteins • understand how amino acids form different types of protein structures and the different methods available for the determination of such structures • appreciate the essentials of experimental design and data presentation, statistical assessment and interpretation • know the main methods of protein purification and characterisation, and the properties of proteins on which these are based • appreciate the uses of molecular biological techniques in biochemical research • understand the use of polyclonal and monoclonal antibodies for detection and quantification of biological molecules • gain an overview of major metabolic pathways in intact cells and tissues • understand the use of isotopes in Biochemistry • be able to read and understand a scientific paper • gain practical experience in spectrophotometry, enzyme assay and column chromatography|
|Key skills provided by module:||writing; presentation; practical skills; organisation; mathematics.|
|Module assessment:||Practical report 10.00%.|
Unseen three-hour written examination 70.00%.
Two tests (45 minutes each) 20.00%.
|Taking this module as an option?:|
|Link to virtual learning environment(registered students only)|
|Last updated:||2014-02-21 13:38:11 by ucbt294|