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UCL Institute of Ophthalmology

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Light microscopes

Info on light microscope types and techniques

Our light microscopes include widefield and laser scanning confocal systems. They are optimised and ready either for fixed or live specimen imaging.

Widefield microscopes

These standard microscopes are used for quick observation and imaging in brightfield (transmitted) or fluorescence modes. The range of magnifications (4x-63x) and fluorescence filter blocks will satisfy most imaging applications. Our unit has two microscopes:

  • Zeiss Axio with a colour camera for fixed specimens and
  • Nikon Ti-E, fully motorised, live cell dedicated system with a sensistive monochrome camera.

Confocal microscopes

Our three systems offer a wide range of imaging techniques to produce high resolution image datasets of fluorescently labelled samples. The Zeiss upright microscopes are optimised for fixed specimens and the Leica SP8 provides fully programmable environmental control for live cell imaging. With these microscopes you can achieve resolutions below diffraction limits, perform FRAP and FRET experiments, do spectral unmixing.

  • Zeiss LSM 700  
  • Zeiss LSM 710
  • Leica SP8 HyVolution