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Antibodies

List of antibodies optimised for mouse brain analysis

Antibody

Stained cells or protein type

Source

Clone

Species  Specificity

.

 .
Antibody       Human Mouse Rat
Bmi1 Polycomb gene product Millipore F6 x x x
BrdU   Abcam BU1/75 (ICR1)   x  
Calbindin Pukinje cells Millipore polyclonal x x x
CD15 Glia Precursor and stem cells BD PharMingen MMA x x  
CD21 Lymphocytes DAKO 1F8   x  
CD3 T-Lymphocytes Invitrogen polyclonal x x  
CD63   Zymed FC-5.01 x    
CITP2   Abcam 25B6 x x  
Clusterin H330   SantaCruz Biotechnology polyclonal x x x
DAB-1 Cell signalling Millipore polyclonal   x  
Doublecortin Stem cell marker Abcam polyclonal   x x
FUS   Novus polyclonal   x  
GFAP Astrocytes DAKO Polyclonal x x x
GFP Green Fluorescent protein Invitrogen C163   x  
HSC-70   SantaCruz Biotechnology B-6 x    
Iba1 Microglia Wako polyclonal   x  
ICSM 18 Abnormal Prion protein D-Gen ICSM 18 x x  
LAMP-1   Abcam polyclonal x x x
LC3   Novus polyclonal x x x
M6PR   Thermo Scientific 2G11 x x x
Map-2 Neurones, Reactive astrocytes Millipore Ap20 x x x
MBP SMI94 Myelin basic protein          
Nestin Neural stem cells Millipore rat-401   x x
Nestin Neural stem cells Abcam 401   x x
Neu-N Neurones Millipore A60 x x x
NF-200 Neurofilaments Sigma NE14 x x  
Olig-2   Abcam polyclonal x x x
p27 Kip1    Cell Signalling Technologies polyclonal x x x
P-Akt   Cell Signalling Technologies D9E x x x
Parvalbumin   Swant polyclonal x x x
PDGRF-a   Abcam polyclonal x x  
Phospho Histone (H3)   Cell Signalling Technologies polyclonal x x x
Phospho-TDP 43   Cosmo Bio Co polyclonal x x  
Proteasome 20S   Enzo MCP79 x x  
PrP 12F10 Abnormal Prion protein   12F10      
PrP 3F4 Abnormal Prion protein   3F4      
PrP ICSM 18 Abnormal and normal Prion protein D-Gen ICSM18      
PrP ICSM 35 Abnormal Prion protein D-Gen  ICSM 35 x x  
PrP KG9 Abnormal Prion protein   KG0      
S-100   DAKO polyclonal x x x
Sox-2   Millipore polyclonal x x  
STMN2   Abnova 1D3 x    
Synaptophysin   Invitrogen Z66 x x  
TBR1   Abcam polyclonal x x x
Tyrosine Hydroxylase   Abcam polyclonal x x x
Ubiquitin   SantaCruz Biotechnology polyclonal x x  
ƴ-Tubulin   Sigma GTU-88 x x  
β-Amyloid (A beta)   DAKO 6F/3D x    
β-Catenin   BD Biosciences 14 x x x
β-Galactosidase   Millipore polyclonal x x x

List of antibodies optimised for human tissue

Antibody

Source

Clone

Species  Specificity
      Human Mouse Rat Other
1C2 (exp polyQ repeat) Chemicon 5TF1-1C2-17.2 x x x  
alpha-Synuclein Abcam KM51 x      
Ab3298 mitichondria Abcam MTC 02 x     Pig
ACTH DAKO Polyclonal x      
ACTH Thermo (LabVision) AH26 x      
APP (A4 Precursor) CHEMICON 22C11 x x x Ca, Mk, Pig, Fsh
beta-A4 DAKO 6F3D x      
bcl2 DAKO 124 x      
Calbindin SWANT D28k-300 x x x  Mk, Gp, Rb, Ck
CD1a DAKO 10 x      
CD3 DAKO Polyclonal x x x  
CD4 VBS 1F6 x     Hu, Bb, Mk
CD8 DAKO C8/144B x      
CD11c VBS 5D11 x      
CD20 Abcam L26 x      
CD30 Abcam BerH2 x      
CD31 Abcam JC/70A x     Rb
CD34 Abcam QBEnd/10 x      
CD45 / LCA Abcam 2B11+PD7/26l x      
CD56 Vector 1B6 x      
CD68 Abcam PG-M1 x      
CD79a Millipore JCB117 x      
CD99 (Mic2) DAKO 1.20E+08 x      
CD138  Millipore  MI 15 x      
CEA Millipore Polyclonal x x x  
Chromogranin VBS 5H7 x      
CK (MNF116) DAKO MNF 116 x x x Rb, Bv
CK (CAM5.2) Becton Dickenson CAM 5.2 x      
CK7 DAKO OV-TL 12/30 x      
CK20 DAKO Ks 20.8 x      
CMV DAKO CCH2 x      
Desmin vector DE-R-11 x x x Dg, Gp, Ck, Hm
Dynorphin Serotec Polyclonal x      
EMA DAKO E29 x      
ER (Oestrogen Rcpt) Abcam 6F 11 x      
Factor VIII DAKO Polyclonal x x   Bv, Hs, Pig
FSH Abcam INN-hFSH-60 x      
GFAP DAKO Polyclonal x x x Fe,Ca,Sh,Bv
GH Abcam W6/32-HL x     Fe, Bv
hCG DAKO polyclonal x x   Ha,Hs,Pig 
HMB45 Abcam HMB45 x      
HSP 70 DAKO Polyclonal x      
HSV I DAKO Polyclonal x      
HSV II DAKO Polyclonal x      
IgA DAKO Polyclonal x      
IgG DAKO Polyclonal x x x many others
IgM DAKO Polyclonal x      
kappa DAKO Polyclonal x      
Ki67 DAKO Mib-1 x x x various mammals
lambda DAKO Polyclonal x     Bv, CA,Hs,Pig
LCA / CD45 DAKO 2B11+PD7/26 x      
LH LabVision-Thermo SPM 103 x      
MAP2 SIGMA HM-2 x x x Ck,Bv
Measles LifeSpan Biosciences 49-21 x     infected cells
Melan A DAKO A103 x      
Mic2 (CD99) DAKO 1.20E+08 x      
MBP (Myelin Basic Prot) DAKO Polyclonal x      
Nestin Millipore Polyclonal x      
NeuN Millipore A60 x x   Av, Fer
NFc  DAKO / Cappel 2F11 x x x Fe, Bv,Ca,Hs,Pig
NFp (phosphorylated) Millipore NP1 x   x Bv, Ck
NF200(RT97) VBS RT97 x      
NSE DAKO BBS/NC/VI-H14 x     Gp
P53 (TSG product) DAKO Do-07 x      
PR Progesterone Rc VBS 16 & SAN 27 x      
PRP ICSM 35 D-Gen  ICSM 35 x x   Sh, Hm, Bv
PRP KG9 Inst animal Health  KG9 x     Bv
Prealbumin (TTR) Abbiotec Polyclonal x x x  
PLAP DAKO 8A9 x      
PSA DAKO ERPR8 x      
Prolactin DAKO Polyclonal x      
S-100 DAKO Polyclonal x x x Fe, Hs,Pig
SMA DAKO IA4 x   x Ck, Bv
SMI 31 Sternberger  SMI 31 x x x Ck
SMI 32 Sternberger SMI 32 x x x mammalian
SOX9  Abcam SOX 9 x      
SV40 / JC Virus Abcam PAb 416 x     SV40 infected cells
Synaptophysin DAKO SY38 x x x Bv,Gp,Rb
Tau (AT8) VBS AT8 x x    
Thyroglobulin VBS 1D4 x      
Toxoplasma Vector TP3 x      
TSH Vector QB2/6 x   x  
TTF1 (Thyroid T F) VBS SPT24 x      
TTR (Transthyretin) DAKO Polyclonal x     Fe, Bv,Ca,Gt, Sh,Pig
UCHL1 (CD45 Ro) DAKO UCHL1 x      
Ubiquitin VBS Polyclonal x      
Vimentin  DAKO V9 x   x Bv,Ha,Ca,Hs,Mk,Rb

List of antibodies optimised for Human tissue (may cross react with mouse and other species)