Neuropathology
Ion



Visitors should report to the security front desk in Queen Square House (Institute of Neurology), the 11-storey tower block on the north corner of Queen Square.

Electron Microscopy

Location: Basement (Room B04), Queen Square House.

The facilities of this inter-departmental Unit are shared with the Institute of Child Health, Great Ormond Street Hospital for Children and the National Hospital for Neurology and Neurosurgery.

The Unit offers comprehensive facilities for specimen preparation and use of Transmission and Scanning Electron Microscopes.

A full technical service is provided for non-EM users from Departments within the host Institutions and from outside.

The Electron Microscope Suite houses the Jeol 1200EX and Jeol 120CX Transmission Electron Microscopes, the Philips CM10 Transmission Electron Microscope and the JSM35 Scanning Electron Microscope. In addition, the adjacent Preparation Laboratory Area provides several ultramicrotome bays and work areas. Lynx automated tissue processors and two recently acquired Reichert-Leica AFS systems are available for the preparation of samples for TEM and SEM examination. The darkroom facility has two Devere Varicon black and white photographic enlargers and an automated print processing system.

The Unit is currently investigating the options available for upgrading the current imaging system to a digital system, but for the foreseeable future, there remains the option for continued use of conventional film and photographic printing facilities.

Contact

Kerrie Venner, Technical Manager

Telephone: 020 344 84287

Email: k.venner@ucl.ac.uk

Dr Mike Groves, Biomedical Scientist

Telephone:  020 344 84220

Email: m.groves@ucl.ac.uk / michael.groves@uclh.nhs.uk

Examples of specimen preparations in our EM Unit

Semi thin resin sections of highest quality
Peripheral nerve; semi thin resin section, MBA-BF stain
Peripheral nerve teased fibre preparation
Peripheral nerve:  teased fibre preparation
Electron microcopy of a peripheral nerve (widely spaced myelin)
Peripheral nerve: Electron microscopy; widely spaced myelin)

Price List

Transmission Electron Microscopy sample preparation charges, includes one hour EM time per sample
Standard machine processing into resin, per run £40
Hand processing into resin, per run £100
Preparation of semi thin section, each block £20
Preparation of set of stained grids, each block £35
Scanning Electron Microscopy sample preparation charges, includes one hour EM time per sample
Machine processing of sample, critical point drying (including consumables and 2 hours SEM examination time), sputter coating with gold £180
With Osmium thiocarbohydrazide treatment £200
Additional time on TEM or SEM and use of ancillary preparation equipment (VAT free)
Per hour £35
Per day £130
Per half day £80
Tuition on TEM and SEM and Processing methods
Per hour £35
Examination of grids by us
Per hour £35
Nerve teasing
Per specimen £35
Morphometry
Per hour £35
Other technical procedures, such as dissection
Per hour £35

Page last modified on 10 jun 12 19:14