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Spectra viewer

Status

Leica SP2 MP: operational (543nm laser not working)
Zeiss LSM 710: fully operational


Confocal microscopy core facility

Laboratory services

Instruments

Inverted Zeiss LSM 710 confocal

Bertrand-Vernay-and-his-amazing-microscope.jpg



A Zeiss LSM 710 inverted confocal microscope with a wide range of excitation lasers.

The Zeiss 710 uses variable spectral detection system instead of traditional emission filters.

The system has 7 laser lines for excitation and any dye that is excited at one of these following wavelengths can be used: 405; 458; 488; 514; 561; 594 and 633 nm (check online or ask Bertrand if you are not sure which dye to use).

Zeiss filter cubes for DAPI, FITC and TRITC (49 DAPI, 43 Cy3, 38 Endow GFP) are installed for standard fluorescence viewing prior to scanning. Transmitted light illumination is available with DIC prisms on all objectives.

Objectives (DIC on all objectives):
Location:

Room 153
1st floor Philip Ullmann Wing
Ext : 2247

Advantages:
  • Z sectioning
  • Good for fixed samples
  • Continuous Spectral imaging (from 390 nm to 750 nm down to 3 nm)
  • Online spectral un-mixing
  • Transmitted light detector (DIC objectives)
  • FRET and FRAP
  • Live imaging (CO2, humidity and temperature controlled chamber)
  • Multi-points experiments  (XYZ positions, tiling)
Cost:
  • User operation £20 per hour
  • User operation £10 per hour of overnight time-lapse
  • Staff operation £50 per hour
  • Training session £50 per hour
Instructions:


Please check the start-up/shut-down procedures and the ZEN 2009 quick start tutorial before starting or alternatively contact Bertrand.

Page last modified on 02 sep 10 17:53 by Bertrand Vernay