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Book on PPMS.
User Guides:
Start-up & shutdown & Objective cleaning.
System Overview:
Upright multiphoton confocal with water dipping long working distance objectives.
7 laser lines for excitation: 405; 458; 488; 514; 561; 594 and 633 nm.
SpectraPhysics Mai Tai eHP DeepSee multiphoton laser. 690-1040nm.
AiryscanFast for 1.7 fold improved resolution 8x improved Signal to noise.
(Check online or ask Dale if you are not sure which dye to use, 2-photon excitation doesn't work well for some fluorophores, such as Alexa647).
Objectives:
- 20x/NA1.0 Plan Apochromat Water dipping objective with correction collar. WD 2.4mm. Amazing working distance given the very high NA. Perfect for live (multiphoton) imaging due to exceptional brightness and resolution. Correction collar for RI differences from 1.33 to 1.36. Also suitable for cleared samples, particularly ScaleA2.
- 10x/NA0.5 W-Plan Apochromat Water dipping objective. WD 3.7mm. UV VIS IR objective with amazing working distance given the high NA. Perfect for live imaging and multiphoton imaging or ablations due to exceptional brightness and sensitivity.
- 10x/NA0.3 EC Plan-Neofluar DIC Dry. WD 5.2mm. Large field of view and very long working distance for deep imaging with standard or 2-photon excitation. Lower NA than the 10x water dipping objective so refractive index mis-match isn't such a problem, and gives a much thicker z slice in multiphoton imaging.
- 2.5x/NA0.085 Plan-Neofluar Dry. WD 8.8mm. Low mag long working distance objective for orienting and manipulating large samples before imaging at higher mag. Suprisingly good imaging objective for large FOV samples.
- 63x/NA1.4 Plan Apo Oil Highest resolution Airyscan imaging available on request. Use with levelling stage.
- 5x/NA0.16 EC Plan-Neofluar. WD 18mm. Low mag long working distance objective for orienting and manipulating large samples before imaging at higher mag. Suprisingly good imaging objective for large FOV samples.
Detectors:
Non Descanned Detectors (Multi-photon) 2 x GaAsP plus 2 standard PMT NDD detectors with filter sets for blue (485 SP), green (500-550 BP), red (575-610 BP) and far red (640-710 BP)
Descanned Detectors (Single photon) 1 x GaAsP plus 2 standard PMT detectors
Features:
- Super-resolution confocal imaging with AiryscanFast
- Deep tissue imaging (RI matched objectives, 2 photon)
- Ablations and dissection with 2-photon laser
- GaAsP internal and external detectors for highest sensitivity
- Z sectioning for 3D imaging
- Transmitted light detector (DIC objectives)
- FRET and FRAP
- Live imaging (CO2, O2, humidity and temperature controlled chamber)
- Multi-point experiments (XYZ positions, tiling)
- Variable stage speed for slow movement of delicate samples
Cost:
- User operation £28 per hour, reduced rate £20 at weekends
- £35 per hour Non-ICH academic rate, £25 weekends
- User operation £10 per hour of overnight time-lapse
- Staff operation £50 per hour
- Training session £50 per hour
