Department of Structural and Molecular Biology

Division of Biosciences

University College London

Darwin Building - Gower Street

WC1E 6BT London, UK



Nicolas Werbeck

PostDoc (FEBS LTF / DFG)


Now PI at Bayer Pharma AG, Berlin, Germany







Research Interests:


I am interested in how proteins interact with other proteins, substrates, ions or other binding partners and how these interactions regulate their function on a molecular level. Currently, I study the structural dynamics and regulation of Histone Deacetylase 8 by NMR.


Brief CV:


2011 – ongoing  Postdoc with D. F. Hansen at University College London, UK

2006- 2010  PhD  with J. Reinstein at Max Planck Institute, Heidelberg, Germany

2005-2006  Diploma Thesis with L.S. Itzhaki, Cambridge, UK

13. Werbeck ND, Kirkpatrick J, Reinstein J, Hansen DF (2014) Using 15N-ammonium to characterise and map potassium binding-sites in proteins by NMR spectroscopy, ChemBioChem, in press


12. Zeymer C, Barends TRM , Werbeck ND, Schlichting I, Reinstein J (2014) Elements in Nucleotide Sensing and Hydrolysis of the AAA+ Disaggregation Machine ClpB – A Structure-Based Mechanistic Dissection of a Molecular Motor, Acta Cryst. D, in press


11. Kunze MB, Wright DW, Werbeck ND, Kirkpatrick J, Coveney PV, Hansen DF (2013) Loop Interactions and Dynamics Tune the Enzymatic Activity of the Human Histone Deacetylase 8. J. Am. Chem. Soc., in press


10. Werbeck ND, Kirkpatrick J, Hansen DF (2013) Probing arginine side-chains and their dynamics with carbon-detected NMR spectroscopy: application to the 42 kDa human histone deacetylase 8 at high pH. Angew. Chem. Int. Ed. Engl. 52, 3145-7


9. Zeymer C, Werbeck ND, Schlichting I, Reinstein J (2013) The molecular mechanism of Hsp100 chaperone inhibition by the prion curing agent guanidinium chloride. J. Biol. Chem., 288, 7065-76


8. Werbeck ND, Zeymer C, Kellner JN, Reinstein J (2011) Coupling of oligomerization and nucleotide binding in the AAA+ chaperone ClpB. Biochemistry, 50, 899-909


7. Barends TR, Werbeck ND, & Reinstein J (2010) Disaggregases in 4 dimensions. Curr. Opin. Struct. Biol., 20, 46-53.


6. Werbeck ND, Kellner JN, Barends TR, & Reinstein J (2009) Nucleotide binding and allosteric modulation of the second AAA+ domain of ClpB probed by transient kinetic studies. Biochemistry, 48, 7240-7250.


5. Takayama Y, Werbeck ND, Komori H, Morita K, Ozawa K, Higuchi Y, & Akutsu H (2008) Strategic roles of axial histidines in structure formation and redox regulation of tetraheme cytochrome c3. Biochemistry, 47, 9405-9415.


4. Werbeck ND*, Rowling PJ*, Chellamuthu VR, & Itzhaki LS (2008) Shifting transition states in the unfolding of a large ankyrin repeat protein. Proc. Natl. Acad. Sci. U. S. A, 105, 9982-9987. *equal contribution


3. Werbeck ND, Schlee S, & Reinstein J (2008) Coupling and dynamics of subunits in the hexameric AAA+ chaperone ClpB. J. Mol. Biol., 378, 178-190.


2. Leskovar A, Wegele H, Werbeck ND, Buchner J, & Reinstein J (2008) The ATPase cycle of the mitochondrial Hsp90 analog Trap1. J. Biol. Chem., 283, 11677-11688.


1. Werbeck ND & Itzhaki LS (2007) Probing a moving target with a plastic unfolding intermediate of an ankyrin-repeat protein. Proc. Natl. Acad. Sci. U. S. A, 104, 7863-7868.