All new users are required to create an account on our online booking system (PPMS) prior to using the facility. Cell analysers may only be used by those who have been trained by facility staff. Users who can demonstrate previous experience with similar analysers may not need full training (at the discretion of Facility Manager) but will still need to be shown the cleaning procedures and have the rules of use of the facility explained.
The UCL Joint GOSICH & IoO flow cytometry facility is open to GOSICH, GOSH, IoO, UCL and external users. To access ZCR site, GOSICH and GOSH users will need to have access to the Zayed Centre for Research Into Rare Disease in Children (ZCR) on their ID cards and and should email their Section/Programme Administrator to arrange this; IoO site is accessible to all IoO staff. UCL and external users will need to call the facility on 020 3978 3602 (ZCR site) or 020 7608 6820 (IoO site) to allow them access.
Users are welcome to book training with us to learn how to use the cell analysers (LSRII, CyAn, FACSArray, FACSCalibur, and CytoFLEX S, LSRFortessa X-20, Magpix Luminex Magpix) correctly.
No training is needed for cell sorting (FACSAriaIII, MoFlo XDP, and BD Influx). Only facility's staff are allowed to operate the cell sorters. Advanced users with cell sorting experience may be allowed to operate the cell sorters under supervision.
We offer operator hands-on training and in-depth principles of flow cytometry course. Training or advanced course can be given at an agreed time when booked in advance and is charged at £60 per hour for GOSICH, GOSH, and IoO users; £85 per hour for UCL users ; and £110 per hour for external (non-UCL) users. The training session usually takes an hour and a half for most users but can be extended to two hours for novice users. Advanced course takes a minimum of 2 hours.
All instruments are available on first-come first-served basis.
Cell analysers can be booked on our PPMS.
Cell sorter can only be booked by email after the first sort has taken place. To book their first sort users are advised to contact in person facility staff and must fill out and sign a biosafety questionnaire form before experiments or projects are started. No sort will take place if this form is not handed to the operator before the first sort is due.