Flow Cytometry Core Facility
Flow Cytometry Core Facility at UCL GOS Institute of Child Health provides state of the art instrumentation and expertise in cell analysis and sorting.
Guidelines for the use of the facility are provided on these web pages. Please ensure you have read the relevant guidelines to maximise the success and validity of your experiment.
Book an instrument or REGISTER
Only PPMS registered users have access to instruments and analysis computers. The facility has two types of insruments and two free of charge analysis computers. The instruments are either cell analysers or cell sorters. Users can directly book any cell analyser they trained on. Cell sorters are only operated and booked by facility's staff. Users can check the availibility of the cell sorters in the link below and call or send an email to facility's staff indicating the time they would like to book their sort.
Check availability of a CELL SORTER*
*must login with firstname.lastname@example.org account - requires PW that is available at the facility. You can only see availability.
BenchSci: Search published antibody data
Make sure to check that the antibody was validated to be used in flow cytometry (FCM).
FluoroFinder: build your FCM panels
The facility has five cell analysers:
The facility offers cell sorting on the latest technology from Beckman Coulter and Becton Dickinson, the high speed cell sorters
- cell sorting
- cloning: single cell sorting
- data acquisition, analysis and interpretation
- user training
- advice on sample preparation
- user assistance and machine troubleshooting
- help with design of experiments
- sourcing of reagents and techniques; finding alternative approaches
- help with data preparation for presentation and publication
We are also open to collaboration.
Funding and Acknowledgements
Please acknowledge use of the facility in all publications. Suggested text: Flow cytometry was performed at the UCL Great Ormond Street Institute of Child Health Flow Cytometry Core Facility, supported by the Great Ormond Street Children's Charity (GOSHCC), grant reference U09822 (October 2007), and UCL Capital Equipment Funding, School of Life and Medical Sciences (September 2012).