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This module will provide an introduction to key molecular contemporary techniques used in cardiovascular research. Students will undertake practical work covering fundamental laboratory skills (pipetting, aseptic techniques etc.) and more advanced genetic/molecular biology methods such as genetic screening, sequencing methods and molecular cloning. This will be complemented by lectures, exercises, e-learning and tutorials on topics related to practical sessions.
Module code: CARD0005
UCL Credits: 15
Module Organiser:Dr Petros Syrris
Learning objectives
Students successfully completing this module should:
- Have practised fundamental laboratory methods
- Have acquired a good theoretical understanding of key laboratory techniques related to cardiovascular research
- Have experienced and be able to use key computer based bioinformatics tools related to cardiovascular research
Timetable
- Wednesdays, 10.00am - 12.00 Midday
- Laboratory sessions Fridays, 10.00 to 13.00 (10 weeks)
Topics
- Introduction and DNA extraction from blood
- DNA extraction from saliva and tissue
- Assessment of DNA quantity/quality
- PCR and agarose gel electrophoresis
- Purification of PCR products and sequencing reaction
- Use of DNA sequencer: set up and loading
- Introduction: plating bacteria
- Molecular cloning
- Restriction endonuclease mapping
Assessment
| Method write up. This will include an overview of method(s) used and critical appraisal (1,000 words) | 50% |
| Unseen exam on theory and data handling (1 hour) | 50% |
Reading list
This is a suggested reading list, students will be expected to engage with additional readings throughout the module.
Key textbook on Genetics:
Key publications:
Metzker ML. Sequencing technologies - the next generation. Nat Rev Genet. 2010;11(1):31-46.
Sonnemans DG, de Windt LJ, de Muinck ED, Doevendans PA. Methods in molecular cardiology: the polymerase chain reaction. Neth Heart J. 2002;10(10):412-418.
Taylor CF, Taylor GR. Current and emerging techniques for diagnostic mutation detection: an overview of methods for mutation detection. Methods Mol Med. 2004;92:9-44.