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Online: CRUK-UCL Centre Proteomics Seminar Series

23 March 2022, 12:00 pm–1:00 pm

CRUK-UCL Centre Proteomics Seminar Prof Bernd Wollscheid,

Prof Bernd Wollscheid, Institute of Translational Medicine, ETH Zurich, Switzerland, presents: 'Light-mediated discovery of surfaceome nanoscale organization and inter-cellular receptor interaction networks.'

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CRUK-UCL Centre

Hosted by Dr Silvia Surinova

Abstract

The molecular nanoscale organization of the surfaceome is a fundamental regulator of cellular signaling in health and disease. Technologies for mapping the spatial relationships of cell surface receptors and their extracellular signaling synapses would unlock theranostic opportunities to target protein communities and the possibility to engineer extracellular signaling. Here, we develop an optoproteomic technology termed LUX-MS that enables the targeted elucidation of acute protein interactions on and in between living cells using light-controlled singlet oxygen generators (SOG). By using SOG-coupled antibodies, small molecule drugs, biologics and intact viral particles, we demonstrate the ability of LUX-MS to decode ligand receptor interactions across organisms and to discover surfaceome receptor nanoscale organization with direct implications for drug action. Furthermore, by coupling SOG to antigens we achieved light-controlled molecular mapping of intercellular signaling within functional immune synapses between antigen-presenting cells and CD8+ T cells providing insights into T cell activation with spatiotemporal specificity. LUX-MS based decoding of surfaceome signaling architectures thereby provides a molecular framework for the rational development of theranostic strategies.

Join Zoom Meeting: https://ucl.zoom.us/j/99974233537?pwd=UDlqZ2lLTm9VbUp2Y094eDRPTGpIZz09

Meeting ID: 999 7423 3537Passcode: 060900

 

About the Speaker

Bernd Wollscheid

The goal of the Wollscheid laboratory is to functionally understand the cellular surfaceome and its signaling islands as a complex information gateway connecting the intracellular to the extracellular interactome. We develop and apply next generation technologies at the interface of biology, chemistry, medicine and bioinformatics to generate unprecedented data to establish the surfaceome proteotype and its signaling interaction network. This digital proteotype data layer provides the basis for generating qualitative and quantitative surfaceome models explaining how molecular nanoscale organization influences cellular signaling and biological function.

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