Antibodies
List of antibodies optimised for mouse brain analysis
Antibody |
Stained cells or protein type |
Source |
Clone |
Species Specificity |
. |
. |
| Antibody | Human | Mouse | Rat | |||
| Bmi1 | Polycomb gene product | Millipore | F6 | x | x | x |
| BrdU | Abcam | BU1/75 (ICR1) | x | |||
| Calbindin | Pukinje cells | Millipore | polyclonal | x | x | x |
| CD15 | Glia Precursor and stem cells | BD PharMingen | MMA | x | x | |
| CD21 | Lymphocytes | DAKO | 1F8 | x | ||
| CD3 | T-Lymphocytes | Invitrogen | polyclonal | x | x | |
| CD63 | Zymed | FC-5.01 | x | |||
| CITP2 | Abcam | 25B6 | x | x | ||
| Clusterin H330 | SantaCruz Biotechnology | polyclonal | x | x | x | |
| DAB-1 | Cell signalling | Millipore | polyclonal | x | ||
| Doublecortin | Stem cell marker | Abcam | polyclonal | x | x | |
| FUS | Novus | polyclonal | x | |||
| GFAP | Astrocytes | DAKO | Polyclonal | x | x | x |
| GFP | Green Fluorescent protein | Invitrogen | C163 | x | ||
| HSC-70 | SantaCruz Biotechnology | B-6 | x | |||
| Iba1 | Microglia | Wako | polyclonal | x | ||
| ICSM 18 | Abnormal Prion protein | D-Gen | ICSM 18 | x | x | |
| LAMP-1 | Abcam | polyclonal | x | x | x | |
| LC3 | Novus | polyclonal | x | x | x | |
| M6PR | Thermo Scientific | 2G11 | x | x | x | |
| Map-2 | Neurones, Reactive astrocytes | Millipore | Ap20 | x | x | x |
| MBP SMI94 | Myelin basic protein | |||||
| Nestin | Neural stem cells | Millipore | rat-401 | x | x | |
| Nestin | Neural stem cells | Abcam | 401 | x | x | |
| Neu-N | Neurones | Millipore | A60 | x | x | x |
| NF-200 | Neurofilaments | Sigma | NE14 | x | x | |
| Olig-2 | Abcam | polyclonal | x | x | x | |
| p27 Kip1 | Cell Signalling Technologies | polyclonal | x | x | x | |
| P-Akt | Cell Signalling Technologies | D9E | x | x | x | |
| Parvalbumin | Swant | polyclonal | x | x | x | |
| PDGRF-a | Abcam | polyclonal | x | x | ||
| Phospho Histone (H3) | Cell Signalling Technologies | polyclonal | x | x | x | |
| Phospho-TDP 43 | Cosmo Bio Co | polyclonal | x | x | ||
| Proteasome 20S | Enzo | MCP79 | x | x | ||
| PrP 12F10 | Abnormal Prion protein | 12F10 | ||||
| PrP 3F4 | Abnormal Prion protein | 3F4 | ||||
| PrP ICSM 18 | Abnormal and normal Prion protein | D-Gen | ICSM18 | |||
| PrP ICSM 35 | Abnormal Prion protein | D-Gen | ICSM 35 | x | x | |
| PrP KG9 | Abnormal Prion protein | KG0 | ||||
| S-100 | DAKO | polyclonal | x | x | x | |
| Sox-2 | Millipore | polyclonal | x | x | ||
| STMN2 | Abnova | 1D3 | x | |||
| Synaptophysin | Invitrogen | Z66 | x | x | ||
| TBR1 | Abcam | polyclonal | x | x | x | |
| Tyrosine Hydroxylase | Abcam | polyclonal | x | x | x | |
| Ubiquitin | SantaCruz Biotechnology | polyclonal | x | x | ||
| ƴ-Tubulin | Sigma | GTU-88 | x | x | ||
| β-Amyloid (A beta) | DAKO | 6F/3D | x | |||
| β-Catenin | BD Biosciences | 14 | x | x | x | |
| β-Galactosidase | Millipore | polyclonal | x | x | x |
List of antibodies optimised for human tissue
Antibody |
Source |
Clone |
Species Specificity |
|||
| Human | Mouse | Rat | Other | |||
| 1C2 (exp polyQ repeat) | Chemicon | 5TF1-1C2-17.2 | x | x | x | |
| alpha-Synuclein | Abcam | KM51 | x | |||
| Ab3298 mitichondria | Abcam | MTC 02 | x | Pig | ||
| ACTH | DAKO | Polyclonal | x | |||
| ACTH | Thermo (LabVision) | AH26 | x | |||
| APP (A4 Precursor) | CHEMICON | 22C11 | x | x | x | Ca, Mk, Pig, Fsh |
| beta-A4 | DAKO | 6F3D | x | |||
| bcl2 | DAKO | 124 | x | |||
| Calbindin | SWANT | D28k-300 | x | x | x | Mk, Gp, Rb, Ck |
| CD1a | DAKO | 10 | x | |||
| CD3 | DAKO | Polyclonal | x | x | x | |
| CD4 | VBS | 1F6 | x | Hu, Bb, Mk | ||
| CD8 | DAKO | C8/144B | x | |||
| CD11c | VBS | 5D11 | x | |||
| CD20 | Abcam | L26 | x | |||
| CD30 | Abcam | BerH2 | x | |||
| CD31 | Abcam | JC/70A | x | Rb | ||
| CD34 | Abcam | QBEnd/10 | x | |||
| CD45 / LCA | Abcam | 2B11+PD7/26l | x | |||
| CD56 | Vector | 1B6 | x | |||
| CD68 | Abcam | PG-M1 | x | |||
| CD79a | Millipore | JCB117 | x | |||
| CD99 (Mic2) | DAKO | 1.20E+08 | x | |||
| CD138 | Millipore | MI 15 | x | |||
| CEA | Millipore | Polyclonal | x | x | x | |
| Chromogranin | VBS | 5H7 | x | |||
| CK (MNF116) | DAKO | MNF 116 | x | x | x | Rb, Bv |
| CK (CAM5.2) | Becton Dickenson | CAM 5.2 | x | |||
| CK7 | DAKO | OV-TL 12/30 | x | |||
| CK20 | DAKO | Ks 20.8 | x | |||
| CMV | DAKO | CCH2 | x | |||
| Desmin | vector | DE-R-11 | x | x | x | Dg, Gp, Ck, Hm |
| Dynorphin | Serotec | Polyclonal | x | |||
| EMA | DAKO | E29 | x | |||
| ER (Oestrogen Rcpt) | Abcam | 6F 11 | x | |||
| Factor VIII | DAKO | Polyclonal | x | x | Bv, Hs, Pig | |
| FSH | Abcam | INN-hFSH-60 | x | |||
| GFAP | DAKO | Polyclonal | x | x | x | Fe,Ca,Sh,Bv |
| GH | Abcam | W6/32-HL | x | Fe, Bv | ||
| hCG | DAKO | polyclonal | x | x | Ha,Hs,Pig | |
| HMB45 | Abcam | HMB45 | x | |||
| HSP 70 | DAKO | Polyclonal | x | |||
| HSV I | DAKO | Polyclonal | x | |||
| HSV II | DAKO | Polyclonal | x | |||
| IgA | DAKO | Polyclonal | x | |||
| IgG | DAKO | Polyclonal | x | x | x | many others |
| IgM | DAKO | Polyclonal | x | |||
| kappa | DAKO | Polyclonal | x | |||
| Ki67 | DAKO | Mib-1 | x | x | x | various mammals |
| lambda | DAKO | Polyclonal | x | Bv, CA,Hs,Pig | ||
| LCA / CD45 | DAKO | 2B11+PD7/26 | x | |||
| LH | LabVision-Thermo | SPM 103 | x | |||
| MAP2 | SIGMA | HM-2 | x | x | x | Ck,Bv |
| Measles | LifeSpan Biosciences | 49-21 | x | infected cells | ||
| Melan A | DAKO | A103 | x | |||
| Mic2 (CD99) | DAKO | 1.20E+08 | x | |||
| MBP (Myelin Basic Prot) | DAKO | Polyclonal | x | |||
| Nestin | Millipore | Polyclonal | x | |||
| NeuN | Millipore | A60 | x | x | Av, Fer | |
| NFc | DAKO / Cappel | 2F11 | x | x | x | Fe, Bv,Ca,Hs,Pig |
| NFp (phosphorylated) | Millipore | NP1 | x | x | Bv, Ck | |
| NF200(RT97) | VBS | RT97 | x | |||
| NSE | DAKO | BBS/NC/VI-H14 | x | Gp | ||
| P53 (TSG product) | DAKO | Do-07 | x | |||
| PR Progesterone Rc | VBS | 16 & SAN 27 | x | |||
| PRP ICSM 35 | D-Gen | ICSM 35 | x | x | Sh, Hm, Bv | |
| PRP KG9 | Inst animal Health | KG9 | x | Bv | ||
| Prealbumin (TTR) | Abbiotec | Polyclonal | x | x | x | |
| PLAP | DAKO | 8A9 | x | |||
| PSA | DAKO | ERPR8 | x | |||
| Prolactin | DAKO | Polyclonal | x | |||
| S-100 | DAKO | Polyclonal | x | x | x | Fe, Hs,Pig |
| SMA | DAKO | IA4 | x | x | Ck, Bv | |
| SMI 31 | Sternberger | SMI 31 | x | x | x | Ck |
| SMI 32 | Sternberger | SMI 32 | x | x | x | mammalian |
| SOX9 | Abcam | SOX 9 | x | |||
| SV40 / JC Virus | Abcam | PAb 416 | x | SV40 infected cells | ||
| Synaptophysin | DAKO | SY38 | x | x | x | Bv,Gp,Rb |
| Tau (AT8) | VBS | AT8 | x | x | ||
| Thyroglobulin | VBS | 1D4 | x | |||
| Toxoplasma | Vector | TP3 | x | |||
| TSH | Vector | QB2/6 | x | x | ||
| TTF1 (Thyroid T F) | VBS | SPT24 | x | |||
| TTR (Transthyretin) | DAKO | Polyclonal | x | Fe, Bv,Ca,Gt, Sh,Pig | ||
| UCHL1 (CD45 Ro) | DAKO | UCHL1 | x | |||
| Ubiquitin | VBS | Polyclonal | x | |||
| Vimentin | DAKO | V9 | x | x | Bv,Ha,Ca,Hs,Mk,Rb |
List of antibodies optimised for Human tissue (may cross react with mouse and other species)