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Do proteins explore the same conformations in the highly crowded environment of the cytosol as in dilute solution? Developing our understanding of protein structure within the cell is important not only for understanding fundamental processes, but also as the basis for improved intervention strategies. We are therefore developing in-cell NMR spectroscopy for the study of the conformational properties of proteins within living cells.

Further reading:

• Rapid distinction of intracellular and extracellular proteins using NMR diffusion measurements. Waudby CA, Mantle M, Cabrita LD, Gladden L, Dobson CM, Christodoulou J. J Am Chem Soc (2012) 134, 11312-11315. pubmed | pdf
• In-cell NMR characterization of the secondary structure populations of a disordered conformation of α-synuclein within E. coli. Waudby CA, Camilloni C, Fitzpatrick AWP, Cabrita LD, Dobson CM, Vendruscolo M, Christodoulou J. PLoS ONE (2013) 8(8), e72286. pubmed | pdf