UCL Division of Biosciences




Closely related Lak megaphages replicate in the microbiomes of diverse animals. Crisci MA, Chen L-X, Devoto AE, Lei S, [...] Banfield JF, Santini JM. iScience

Anthroponosis and risk management: a time for ethical vaccination of wildlife? Edwards SJL, Chatterjee HJ, Santini JM. Lancet Microbe.

Structural and functional investigation of the periplasmic arsenate-binding protein ArrX from Chrysiogenes arsenatisPoddar N, Badilla C, Maghool S, Osborne TH, Santini JM, Maher MJ. Biochemistry. 60, 465.

Phylogenomics reveals the basis of adaptation of Pseudorhizobium species to extreme environments and supports a taxonomic revision of the genus. Lasalle F, Dastgheib SMM, [...] Santini JM, Petersen J. Syst. Appl. Microbiol. 44, 126165.


SARS-CoV-2 spike protein predicted to form complexes with host receptor protein orthologues from a broad range of mammals. Lam SD, Bordin N, [...] Santini JM, Orengo CA. Sci. Rep. 10, 16471.

Anthroponotic risk of SARS-CoV-2, precautionary mitigation, and outbreak management. Edwards SJ, Santini JM. Lancet Microbe.

Host range of SARS-CoV-2 and implications for public health. Santini JM, Edwards SJ. Lancet Microbe.

Large-scale network analysis captures biological features of bacterial plasmids. Acman M, van Dorp L, Santini JM, Balloux F. Nature Comm. 11: 2452.

Clades of huge phages from across Earth’s ecosystems. Al-Shayeb B, Sachdeva R, [....] Santini JM, Banfield JF. Nature.


Hydrogen-based metabolism as an ancestral trait in lineages sibling to the Cyanobacteria. Matheus Carnevali P, Schulz F, Castelle CJ, Kantor RS, Shih PM, Sharon I, Santini JM, [....] Banfield JF. Nature Comm

Megaphages infect Prevotella and variants are widespread in gut microbiomes. Devoto A, Santini JM, .... Banfield JF. Nature Microbiol.


Structural and mechanistic analysis of the arsenate respiratory reductase provides insight into environmental arsenic transformations. Glasser NR, Oyala PH, Osborne TH, Santini JM, Newman DK. Proc. Nat. Acad. Sci. 

Reservoirs of faecal indicator bacteria in well-head hand pumps in Bangladesh. Osborne TH, Ward SA, Ahmed KM, Santini JM. J. Water & Health. wh2018042. 

A new family of periplasmic-binding proteins involved in binding arsenic oxyanions. Badilla C, Osborne TH, Cole A, Watson C, Djordjevic S, Santini JM. Sci. Rep. 8:6282.

Expression of the arsenite oxidation regulatory operon in Rhizobium sp. str. NT-26 is under the control of two promoters that respond to different environmental cues. Corsini PM., Walker KT, Santini JM. MicrobobiologyOpen.


Electron transfer through arsenite oxidase: insights into Rieske interaction with cytochrome c. Watson C, Niks D, Hille R, Vieira M, Schoepp-Cothenet B, Marques AT, Romao M, Santos-Silva T, Santini JM. Biochim. Biophys. Acta - Bioenergetics. 1858: 865-872.

The active site structure and catalytic mechanism of arsenite oxidase. Warelow TP, Pushie MJ, Cotelesage JJH, Santini JM, George GN. Sci. Rep. 7(1757):


Structure of the bacterial sex F pilus reveals an assembly of a stoichiometric protein-phospholipid complex. Costa TRD, Ilangovan A, Ukleja M, Redzej A, Santini JM, Smith TK, Egelman EH, Waksman G. Cell 166: 1436-1444.

The H-bond network surrounding the pyranopterins modulates redox cooperativity in the molybdenum-bisPGD cofactor in arsenite oxidase. Duval S, Santini JM, Lemaire D, Chaspoul F, Russell MJ, Nistchke W, Schoepp-Cothenet B. Biochim. Biophys. Acta - Bioenergetics 1857: 1353-1362.

Comparative proteomics of Acidithiobacillus ferrooxidans grown in the presence and absence of uranium. Dekker L, Arsène-Ploetze F, Santini JM. Res. Microbiol. 167(3): 234-239.


Isolation of an arsenate-respiring bacterium from a redox front in an arsenic-polluted aquifer in West Bengal, Bengal Basin. Osborne TH, McArthur JM, Sikdar PK, Santini JM.  Environ. Sci. Technol. 49(7): 4193-4199.

Arsenite oxidase also functions as an antimonite oxidase. Wang Q*, Warelow TP*, Kang Y-S, Romano C, Osborne TH, Lehr CR, Bothner B, McDermott TR^, Santini JM^, Wang G^.  Appl. Environ. Microbiol. 81(6): 1959-1965. * contributed equally; ^ corresponding authors


Electrochemically driven catalysis of Rhizobium sp. NT-26 arsenite oxidase with its native electron acceptor cytochrome c552. Kalimuthu P, Heath MD, Santini JM, Kappler U, Bernhardt PV. Biochim. Biophys. Acta -Bioenergetics. 1837(1): 112-120.

Isolation and identification of cobalt and caesium resistant bacteria from a nuclear fuel storage pond. Dekker L, Osborne TH, Santini JM. FEMS Microbiol. Lett. 359(1): 81-84.


The respiratory arsenite oxidase: structure and the role of residues surrounding the Rieske cluster. Warelow TP, Oke M, Schoepp-Cothenet B, Dahl JU, Bruselat N, Sivalingam GN, Leimkühler S, Thalassinos K, Kappler U, Naismith JH, Santini JM.  PLoS One 8(8): e72535.

Life in an arsenic-containing gold mine: genome and physiology of the autotrophic arsenite-oxidizing bacterium Rhizobium sp. NT-26. Andres J, Arsène-Ploetze F, Barbe V, Brochier-Armanet C, Cleiss-Arnold J, Coppée JY, Dillies MA, Geist L, Joublin A, Koechler S, Lassalle F, Marchal M, Médigue C, Muller D, Nesme X, Plewniak F, Proux C, Ramirez-Bahena MH, Schenowitz C, Sismeiro O, Vallenet D, Santini JM, Bertin PN.  Gen. Biol. Evol. 5(5): 934-953.

Cold-adapted arsenite oxidase from a psychrotolerant Polaromonas species. Osborne TH, Heath MD, Martin ACR, Pankowski JA, Hudson-Edwards KA, Santini JM (2013).  Metallomics 5(4): 318-324.

Arsenic-microbe-mineral interactions in mining-affected environments. Hudson-Edwards KA, Santini JM. Minerals 3: 337-351.


Heterologously expressed arsenite oxidase: A system to study biogenesis and structure/function relationships of the enzyme family. van Lis R, Nitschke W, Warelow TP, Capowiez L, Santini JM, Schoepp-Cothenet B.  Biochim. Biophys. Acta - Bioenergetics 1817(9): 1701-1708.

Arsenite oxidase, in The Metabolism of Arsenite. Heath MD, Schoepp-Cothenet B, Osborne TH, Santini JM. eds. Santini JM & Ward SA, CRC press. pp. 81-97.

Prokaryotic aerobic oxidation of arsenite, in The Metabolism of Arsenite. Osborne TH, Santini JM. eds. Santini JM & Ward SA, CRC press. pp. 61-72.

Unified nomenclature for genes involved in prokaryotic aerobic arsenite oxidation. Lett MC, Muller D, Lièvremont D, Silver S, Santini JM. J. Bacteriol. 194(2): 207-208.


Characterization of a two-component signal transduction system that controls arsenite oxidation in the chemolithoautotroph NT-26. Sardiwal S, Santini JM, Osborne TH, Djordjevic S. FEMS Microbiol. Lett. 313(1): 20-28.

Microbial oxidation of arsenite in a subartic environment: Diversity of arsenite oxidase genes and identification of a psychrotolerant arsenite oxidiser. Osborne TH, Jamieson HE, Hudson-Edwards KA, Nordstrom DK, Walker SR, Ward SA, Santini JM. BMC Microbiol. 10(205).

The small subunit AroB of arsenite oxidase: lessons on the [2Fe-2S]-Rieske protein superfamily. Duval S, Santini JM, Nitschke W, Hille R, Schoepp-Cothenet B. J. Biol. Chem. 285(27): 20442-20451.

Quinol-cytochrome c oxidoreductase and cytochrome c4 mediate electron transfer during selenate respiration in Thauera selenatis. Lowe EC, Bydder S, Hartshorne RS, Tape HL, Dridge EJ, Debieux CM, Paszkiewicz K, Singleton I, Lewis RJ, Santini JM, Richardson DJ, Butler CS. J. Biol. Chem. 285(24): 18433-18442.


Comment on "Arsenic (III) fuels anoxygenic photosynthesis in hot spring biofilms from Mono Lake, California". Schoepp-Cothenet B, Duval S, Santini JM, Nitschke W. Science 323(5914): 583.


Investigation of the redox centres of selenate reductase from Thauera selenatis by electron paramagnetic resonance spectroscopy. Dridge EJ, Watts CA, Jepson BJN, Line K, Santini JM, Richardson DJ, Butler CS. Biochem. J. 408(1): 19-28.

Alkalilimnicola ehrlichii sp. nov., a novel arsenite-oxidizing, haloalkaliphilic gammaproteobacterium capable of chemoautotrophic or heterotrophic growth with nitrate or oxygen as the electron acceptor. Hoeft SE, Switzer Blum J, Stolz JF, Tabita FR, Witte B, King GM, Santini JM, Oremland RS. Int. J. Syst. Evol. Microbiol. 570(3): 504-512.

Detection, diversity and expression of aerobic bacterial arsenite oxidase genes. Inskeep WP, Macur RE, Hamamura N, Warelow TP, Ward SA, Santini JM. Environ. Microbiol. 9(4): 934-43.

A biosensor for arsenite using arsenite oxidase and multi-walled carbon nanotube modified electrodes. Male KB, Hrapovic S, Santini JM, Luong JHT. Anal. Chem. 79(20): 7831-7837.

The NT-26 cytochrome c552 and its role in arsenite oxidation. Santini JM, Kappler U, Ward SA, Honeychurch MJ, vanden Hoven RN, Bernhardt PV. Biochim. Biophys. Acta - Bioenergetics 1767(2): 189-196.


Protein film voltammetry of arsenite oxidase from the chemolithoautotrophic arsenite-oxidizing bacterium NT-26. Bernhardt PV, Santini JM. Biochemistry 45(9): 2804-2809.

The Rieske protein: a case study on the pitfalls of multiple sequence alignments and phylogenetic reconstruction. Lebrun E, Santini JM, Brugna M, Ducluzeau AL, Ouchane S, Schoepp-Cothenet B, Baymann F, Nitschke W. Mol. Biol. Evol. 23(6): 1180-1191.

Arsenic and selenium in microbial metabolism. Stolz JF, Basu P, Santini JM, Oremland RS.  Ann. Rev. Microbiol. 60: 107-130.


X-ray absorption spectroscopy of selenate reductase. Maher MJ, Santini JM, Pickering IJ, Prince RC, Macy JM, George GN. Inorg. Chem. 43(2): 402-404.

arrA is a specific marker for As(V)-respiration in the environment. Malasarn D, Saltikov CW, Campbell KM, Santini JM, Hering JG, Newman DK. Science 306(5695): 455.

Molybdenum-containing arsenite oxidase of the chemolithoautotrophic arsenite oxidizer NT-26. Santini JM, vanden Hoven RN. J. Bacteriol. 186(6): 1614-1619.

Prokaryotic arsenate and selenate respiration, in Strict and Facultative Anaerobes: Medical and Environmental Aspects. Santini JM, Stolz JF. eds. Nakano MM & Zuber P, CRC Press. pp. 239-250.

Bacillus macyae sp. nov., an arsenate-respiring bacterium isolated from an Australian gold mine. Santini JM, Streimann ICA, vanden Hoven RN. Int. J. Syst. Evol. Microbiol. 54(6): 2241-2244.

Arsenite oxidation by the heterotroph Hydrogenophaga sp. str. NT-14: the arsenite oxidase and its physiological electron acceptor. vanden Hoven RN, Santini JM. Biochim. Biophys. Acta - Bioenergetics 1656(2-3): 148-155.


Unique modes of arsenate respiration by Chrysiogenes arsenatis and Desulfomicrobium sp. str. Ben-RB, in Environmental Chemistry of Arsenic. Macy JM, Santini JM. eds. Frankenberger WT & Dekker M. pp. 297-312. 

Anaerobic oxidation of arsenite in Mono Lake water and by a facultative, arsenite-oxidizing chemoautotroph, strain MLHE-1. Oremland RS, Hoeft SE, Santini JM, Bano N, Hollibaugh RA, Hollibaugh JT. Appl. Environ. Microbiol. 68(10): 4795-5802.

Characteristics of newly discovered arsenite-oxidizing bacteria, in Environmental Chemistry of Arsenic Santini JM, vanden Hoven RN, Macy JM. eds. Frankenberger WT & Dekker M. pp. 329-342.

New arsenite-oxidizing bacteria isolated from Australian gold-mining environments - phylogenetic relationships. Santini JM, Sly LI, Wen A, Comrie D, DeWulf-Durand P, Macy JM. Geomicrobiol. J. 19(1): 67-76.

Isolation of a new arsenate-respiring bacterium - physiological and phylogenetic studies. Santini JM, Stolz JF, Macy JM. Geomicrobiol. J. 19(1): 41-52.


Two new arsenate/sulfate-reducing bacteria: mechanisms of arsenate reduction. Macy JM, Santini JM, Pauling BV, O'Neill A, Sly LI.  Arch. Microbiol. 173(1): 49-57.

A new chemolithoautotrophic arsenite-oxidizing bacterium isolated from a gold mine: phylogenetic, physiological, and preliminary biochemical studies. Santini JM, Sly LI, Schnagl RD, Macy JM. Appl. Environ. Microbiol. 66(1): 92-97.